The conditions of obtaining and spectral-luminescence properties of mixed Langmuir-Blodgett (LB) films of the new regular polyampholyte modified with octadecyl chains and two amphiphilic fluorophores (I and II - rhodamine dyes) were investigated. On the basis of conformational molecule analysis in force field MM2 the models of the dyes molecule and polymer repeat unit orientations at the water-air interface and LB films architecture have been offered. It was found that dye aggregation took place in the densely packed multilayer films, which appeared in absorption and fluorescence spectra. Assumption was made about structure of aggregates. It was established that LB films, for which the ratio of dye and polymer was 17:83 mol%, had the highest fluorescence quantum yield.