Abstract
Mineralized hydrogels are increasingly gaining attention as biomaterials for bone regeneration. The most common mineralization strategy has been addition of preformed inorganic particles during hydrogel formation. This maintains injectability. One common form of bone cement is formed by mixing particles of the highly reactive calcium phosphate alpha-tricalcium phosphate (α-TCP) with water to form hydroxyapatite (HA). The calcium ions released during this reaction can be exploited to crosslink anionic, calcium-binding polymers such as the polysaccharide gellan gum (GG) to induce hydrogel formation. In this study, three different amounts of α-TCP particles were added to GG polymer solution to generate novel, injectable hydrogel-inorganic composites. Distribution of the inorganic phase in the hydrogel was studied by high resolution microcomputer tomography (µCT). Gelation occurred within 30 min. α-TCP converted to HA. µCT revealed inhomogeneous distribution of the inorganic phase in the composites. These results demonstrate the potential of the composites as alternatives to traditional α-TCP bone cement and pave the way for incorporation of biologically active substances and in vitro and in vivo testing.
| Original language | English |
|---|---|
| Pages (from-to) | 822-828 |
| Number of pages | 7 |
| Journal | Journal of Biomedical Materials Research - Part A |
| Volume | 106 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1 Mar 2018 |
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Keywords
- bone cement
- composite
- gellan gum
- hydrogel
- micro-CT
ASJC Scopus subject areas
- Ceramics and Composites
- Biomaterials
- Biomedical Engineering
- Metals and Alloys
Cite this
Novel self-gelling injectable hydrogel/alpha-tricalcium phosphate composites for bone regeneration : Physiochemical and microcomputer tomographical characterization. / Douglas, Timothy E.L.; Schietse, Josefien; Zima, Aneta; Gorodzha, Svetlana; Parakhonskiy, Bogdan V.; KhaleNkow, Dmitry; Shkarin, Roman; Ivanova, Anna; Baumbach, Tilo; Weinhardt, Venera; Stevens, Christian V.; Vanhoorne, Valérie; Vervaet, Chris; Balcaen, Lieve; Vanhaecke, Frank; Slośarczyk, Anna; Surmeneva, Maria A.; Surmenev, Roman A.; Skirtach, Andre G.
In: Journal of Biomedical Materials Research - Part A, Vol. 106, No. 3, 01.03.2018, p. 822-828.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Novel self-gelling injectable hydrogel/alpha-tricalcium phosphate composites for bone regeneration
T2 - Physiochemical and microcomputer tomographical characterization
AU - Douglas, Timothy E.L.
AU - Schietse, Josefien
AU - Zima, Aneta
AU - Gorodzha, Svetlana
AU - Parakhonskiy, Bogdan V.
AU - KhaleNkow, Dmitry
AU - Shkarin, Roman
AU - Ivanova, Anna
AU - Baumbach, Tilo
AU - Weinhardt, Venera
AU - Stevens, Christian V.
AU - Vanhoorne, Valérie
AU - Vervaet, Chris
AU - Balcaen, Lieve
AU - Vanhaecke, Frank
AU - Slośarczyk, Anna
AU - Surmeneva, Maria A.
AU - Surmenev, Roman A.
AU - Skirtach, Andre G.
PY - 2018/3/1
Y1 - 2018/3/1
N2 - Mineralized hydrogels are increasingly gaining attention as biomaterials for bone regeneration. The most common mineralization strategy has been addition of preformed inorganic particles during hydrogel formation. This maintains injectability. One common form of bone cement is formed by mixing particles of the highly reactive calcium phosphate alpha-tricalcium phosphate (α-TCP) with water to form hydroxyapatite (HA). The calcium ions released during this reaction can be exploited to crosslink anionic, calcium-binding polymers such as the polysaccharide gellan gum (GG) to induce hydrogel formation. In this study, three different amounts of α-TCP particles were added to GG polymer solution to generate novel, injectable hydrogel-inorganic composites. Distribution of the inorganic phase in the hydrogel was studied by high resolution microcomputer tomography (µCT). Gelation occurred within 30 min. α-TCP converted to HA. µCT revealed inhomogeneous distribution of the inorganic phase in the composites. These results demonstrate the potential of the composites as alternatives to traditional α-TCP bone cement and pave the way for incorporation of biologically active substances and in vitro and in vivo testing.
AB - Mineralized hydrogels are increasingly gaining attention as biomaterials for bone regeneration. The most common mineralization strategy has been addition of preformed inorganic particles during hydrogel formation. This maintains injectability. One common form of bone cement is formed by mixing particles of the highly reactive calcium phosphate alpha-tricalcium phosphate (α-TCP) with water to form hydroxyapatite (HA). The calcium ions released during this reaction can be exploited to crosslink anionic, calcium-binding polymers such as the polysaccharide gellan gum (GG) to induce hydrogel formation. In this study, three different amounts of α-TCP particles were added to GG polymer solution to generate novel, injectable hydrogel-inorganic composites. Distribution of the inorganic phase in the hydrogel was studied by high resolution microcomputer tomography (µCT). Gelation occurred within 30 min. α-TCP converted to HA. µCT revealed inhomogeneous distribution of the inorganic phase in the composites. These results demonstrate the potential of the composites as alternatives to traditional α-TCP bone cement and pave the way for incorporation of biologically active substances and in vitro and in vivo testing.
KW - bone cement
KW - composite
KW - gellan gum
KW - hydrogel
KW - micro-CT
UR - http://www.scopus.com/inward/record.url?scp=85036564876&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85036564876&partnerID=8YFLogxK
U2 - 10.1002/jbm.a.36277
DO - 10.1002/jbm.a.36277
M3 - Article
VL - 106
SP - 822
EP - 828
JO - Journal of Biomedical Materials Research - Part A
JF - Journal of Biomedical Materials Research - Part A
SN - 1549-3296
IS - 3
ER -