Novel concepts of "niche-relief" and "niche-voltage" for stem cells as a base of bone and hematopoietic tissues biomimetic engineering

Igor Albertovich Khlusov, Yu Dekhtyar, M. Yu Khlusova, E. A. Gostischev, Yu P. Sharkeev, V. F. Pichugin, E. V. Legostaeva

Research output: Chapter in Book/Report/Conference proceedingConference contribution

8 Citations (Scopus)

Abstract

An affect of relief features and quantitative variables of artificial surfaces on structural-functional status of human lung prenatal stromal cells (HLPSC) and remodeling of Balb/c mice bone/bone marrow system have been studied. Implants with rough (Ra>2 μm) calcium phosphate micro-arc coatings have structural-functional sites (micro-regions) named "niches-relief" which are necessary for maturation and differentiation of HLPSC into secreting osteoblasts in shortterm culture. Maximal remodeling of mouse bone/bone marrow system in 45-day subcutaneous heterotopic test in vivo is also noted under optimal parameter (average index of cellular alkaline phosphatase area to artificial micro-region area is about 43 %) of osteogenic niche in vitro. Probable physical mechanism of osteogenic niche functioning has been determined. It correlates with increasing of electron work function supplied with a negative charge of calcium phosphate nanorelief in the sockets (artificial "niches"). "Niche-voltage" concept for bone tissue biomimetic engineering was proposed.

Original languageEnglish
Title of host publicationIFMBE Proceedings
Pages99-102
Number of pages4
Volume38 IFMBE
DOIs
Publication statusPublished - 2013
EventInternational Symposium on Biomedical Engineering and Medical Physics, ISBEMP 2012 - Riga, Latvia
Duration: 10 Oct 201212 Oct 2012

Other

OtherInternational Symposium on Biomedical Engineering and Medical Physics, ISBEMP 2012
CountryLatvia
CityRiga
Period10.10.1212.10.12

Fingerprint

Biomimetics
Stem cells
Bone
Tissue
Electric potential
Calcium phosphate
Osteoblasts
Phosphatases
Cell culture
Alkaline Phosphatase
Coatings
Electrons
calcium phosphate

Keywords

  • artificial microterritories
  • Calcium phosphate coatings
  • cell culture
  • electron work function
  • mice

ASJC Scopus subject areas

  • Biomedical Engineering
  • Bioengineering

Cite this

Novel concepts of "niche-relief" and "niche-voltage" for stem cells as a base of bone and hematopoietic tissues biomimetic engineering. / Khlusov, Igor Albertovich; Dekhtyar, Yu; Khlusova, M. Yu; Gostischev, E. A.; Sharkeev, Yu P.; Pichugin, V. F.; Legostaeva, E. V.

IFMBE Proceedings. Vol. 38 IFMBE 2013. p. 99-102.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Khlusov, IA, Dekhtyar, Y, Khlusova, MY, Gostischev, EA, Sharkeev, YP, Pichugin, VF & Legostaeva, EV 2013, Novel concepts of "niche-relief" and "niche-voltage" for stem cells as a base of bone and hematopoietic tissues biomimetic engineering. in IFMBE Proceedings. vol. 38 IFMBE, pp. 99-102, International Symposium on Biomedical Engineering and Medical Physics, ISBEMP 2012, Riga, Latvia, 10.10.12. https://doi.org/10.1007/978-3-642-34197-7_26
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AU - Khlusova, M. Yu

AU - Gostischev, E. A.

AU - Sharkeev, Yu P.

AU - Pichugin, V. F.

AU - Legostaeva, E. V.

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