Novel concepts of "niche-relief" and "niche-voltage" for stem cells as a base of bone and hematopoietic tissues biomimetic engineering

Igor Albertovich Khlusov, Yu Dekhtyar, M. Yu Khlusova, E. A. Gostischev, Yu P. Sharkeev, V. F. Pichugin, E. V. Legostaeva

Research output: Chapter in Book/Report/Conference proceedingConference contribution

8 Citations (Scopus)


An affect of relief features and quantitative variables of artificial surfaces on structural-functional status of human lung prenatal stromal cells (HLPSC) and remodeling of Balb/c mice bone/bone marrow system have been studied. Implants with rough (Ra>2 μm) calcium phosphate micro-arc coatings have structural-functional sites (micro-regions) named "niches-relief" which are necessary for maturation and differentiation of HLPSC into secreting osteoblasts in shortterm culture. Maximal remodeling of mouse bone/bone marrow system in 45-day subcutaneous heterotopic test in vivo is also noted under optimal parameter (average index of cellular alkaline phosphatase area to artificial micro-region area is about 43 %) of osteogenic niche in vitro. Probable physical mechanism of osteogenic niche functioning has been determined. It correlates with increasing of electron work function supplied with a negative charge of calcium phosphate nanorelief in the sockets (artificial "niches"). "Niche-voltage" concept for bone tissue biomimetic engineering was proposed.

Original languageEnglish
Title of host publicationIFMBE Proceedings
Number of pages4
Volume38 IFMBE
Publication statusPublished - 2013
EventInternational Symposium on Biomedical Engineering and Medical Physics, ISBEMP 2012 - Riga, Latvia
Duration: 10 Oct 201212 Oct 2012


OtherInternational Symposium on Biomedical Engineering and Medical Physics, ISBEMP 2012



  • artificial microterritories
  • Calcium phosphate coatings
  • cell culture
  • electron work function
  • mice

ASJC Scopus subject areas

  • Biomedical Engineering
  • Bioengineering

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