Novel biomimetic thermosensitive β-tricalcium phosphate/chitosan-based hydrogels for bone tissue engineering

M. Dessì, A. Borzacchiello, Tawheed H A Mohamed, Wafa I. Abdel-Fattah, L. Ambrosio

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Among the less invasive surgical procedures for tissue engineering application, injectable in situ gelling systems have gained great attention. In this contest, this article is aimed to realize thermosensitive chitosan-based hydrogels, crosslinked with β-glycerophosphate and reinforced via physical interactions with β-tricalcium phosphate. The kinetics of sol-gel transition and the composite hydrogel properties were investigated by rheological analysis. The hydrogels were also characterized by Fourier transform infrared study, X-ray diffraction, scanning electron microscopy, transmission electron microscopy analysis, and thermal and biological studies. The hydrogels exhibit a gel-phase transition at body temperature, and a three-dimensional network with typical rheological properties of a strong gel. The presence of the inorganic phase, made up of nanocrystals, provides a structure with chemico-physical composition that mimics natural bone tissue, favoring cellular activity. These findings suggest the potential of the materials as promising candidates for hard tissue regeneration. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A: 2984-2993, 2013.

Original languageEnglish
Pages (from-to)2984-2993
Number of pages10
JournalJournal of Biomedical Materials Research - Part A
Volume101
Issue number10
DOIs
Publication statusPublished - 1 Oct 2013
Externally publishedYes

Fingerprint

Hydrogels
Chitosan
Biomimetics
Tissue engineering
Bone
Phosphates
Gels
Glycerophosphates
Tissue regeneration
Hydrogel
Nanocrystals
Sol-gels
Fourier transforms
Phase transitions
Tissue
Transmission electron microscopy
Infrared radiation
X ray diffraction
Scanning electron microscopy
Kinetics

Keywords

  • β-TCP nanopowders
  • bone tissue engineering
  • crosslinked chitosan
  • thermosensitive hydrogels

ASJC Scopus subject areas

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

Cite this

Novel biomimetic thermosensitive β-tricalcium phosphate/chitosan-based hydrogels for bone tissue engineering. / Dessì, M.; Borzacchiello, A.; Mohamed, Tawheed H A; Abdel-Fattah, Wafa I.; Ambrosio, L.

In: Journal of Biomedical Materials Research - Part A, Vol. 101, No. 10, 01.10.2013, p. 2984-2993.

Research output: Contribution to journalArticle

@article{cd60e22326e8493d914ff4be8171c955,
title = "Novel biomimetic thermosensitive β-tricalcium phosphate/chitosan-based hydrogels for bone tissue engineering",
abstract = "Among the less invasive surgical procedures for tissue engineering application, injectable in situ gelling systems have gained great attention. In this contest, this article is aimed to realize thermosensitive chitosan-based hydrogels, crosslinked with β-glycerophosphate and reinforced via physical interactions with β-tricalcium phosphate. The kinetics of sol-gel transition and the composite hydrogel properties were investigated by rheological analysis. The hydrogels were also characterized by Fourier transform infrared study, X-ray diffraction, scanning electron microscopy, transmission electron microscopy analysis, and thermal and biological studies. The hydrogels exhibit a gel-phase transition at body temperature, and a three-dimensional network with typical rheological properties of a strong gel. The presence of the inorganic phase, made up of nanocrystals, provides a structure with chemico-physical composition that mimics natural bone tissue, favoring cellular activity. These findings suggest the potential of the materials as promising candidates for hard tissue regeneration. {\circledC} 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A: 2984-2993, 2013.",
keywords = "β-TCP nanopowders, bone tissue engineering, crosslinked chitosan, thermosensitive hydrogels",
author = "M. Dess{\`i} and A. Borzacchiello and Mohamed, {Tawheed H A} and Abdel-Fattah, {Wafa I.} and L. Ambrosio",
year = "2013",
month = "10",
day = "1",
doi = "10.1002/jbm.a.34592",
language = "English",
volume = "101",
pages = "2984--2993",
journal = "Journal of Biomedical Materials Research - Part A",
issn = "1549-3296",
publisher = "John Wiley and Sons Inc.",
number = "10",

}

TY - JOUR

T1 - Novel biomimetic thermosensitive β-tricalcium phosphate/chitosan-based hydrogels for bone tissue engineering

AU - Dessì, M.

AU - Borzacchiello, A.

AU - Mohamed, Tawheed H A

AU - Abdel-Fattah, Wafa I.

AU - Ambrosio, L.

PY - 2013/10/1

Y1 - 2013/10/1

N2 - Among the less invasive surgical procedures for tissue engineering application, injectable in situ gelling systems have gained great attention. In this contest, this article is aimed to realize thermosensitive chitosan-based hydrogels, crosslinked with β-glycerophosphate and reinforced via physical interactions with β-tricalcium phosphate. The kinetics of sol-gel transition and the composite hydrogel properties were investigated by rheological analysis. The hydrogels were also characterized by Fourier transform infrared study, X-ray diffraction, scanning electron microscopy, transmission electron microscopy analysis, and thermal and biological studies. The hydrogels exhibit a gel-phase transition at body temperature, and a three-dimensional network with typical rheological properties of a strong gel. The presence of the inorganic phase, made up of nanocrystals, provides a structure with chemico-physical composition that mimics natural bone tissue, favoring cellular activity. These findings suggest the potential of the materials as promising candidates for hard tissue regeneration. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A: 2984-2993, 2013.

AB - Among the less invasive surgical procedures for tissue engineering application, injectable in situ gelling systems have gained great attention. In this contest, this article is aimed to realize thermosensitive chitosan-based hydrogels, crosslinked with β-glycerophosphate and reinforced via physical interactions with β-tricalcium phosphate. The kinetics of sol-gel transition and the composite hydrogel properties were investigated by rheological analysis. The hydrogels were also characterized by Fourier transform infrared study, X-ray diffraction, scanning electron microscopy, transmission electron microscopy analysis, and thermal and biological studies. The hydrogels exhibit a gel-phase transition at body temperature, and a three-dimensional network with typical rheological properties of a strong gel. The presence of the inorganic phase, made up of nanocrystals, provides a structure with chemico-physical composition that mimics natural bone tissue, favoring cellular activity. These findings suggest the potential of the materials as promising candidates for hard tissue regeneration. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A: 2984-2993, 2013.

KW - β-TCP nanopowders

KW - bone tissue engineering

KW - crosslinked chitosan

KW - thermosensitive hydrogels

UR - http://www.scopus.com/inward/record.url?scp=84883223489&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84883223489&partnerID=8YFLogxK

U2 - 10.1002/jbm.a.34592

DO - 10.1002/jbm.a.34592

M3 - Article

VL - 101

SP - 2984

EP - 2993

JO - Journal of Biomedical Materials Research - Part A

JF - Journal of Biomedical Materials Research - Part A

SN - 1549-3296

IS - 10

ER -