Kaedah titrimetrik bukan akues novel dan ditentusahkan bagi penentuan penahanan kalium-dadah diuretik dalam formulasi tulen dan farmaseutikal

Translated title of the contribution: Novel and validated non-aqueous titrimetric method for determination of perspective potassium-sparing diuretic drug candidate in pure form and pharmaceutical formulation

Alexey Ivanov, Ivan Smırnov, Tatyana Murashko, Marina Trusova, Elena Stepanova

Research output: Contribution to journalArticlepeer-review

Abstract

4-O-β-D-(glucopyranosyloxy)-benzoic acid sodium salt (4-GBA) is promising potassium-sparing diuretic and anti-inflammatory drug. For the survey of 4-GBA as pharmaceutical substance, a durable method of its quantification is needed. In the present study, a titrimetric method for 4-GBA quantification since it is evident, very simple, fast, applicable for routine analysis and there is no need of using reference standards was developed. Aqueous titration gave unsatisfactory result. Thus, based on the weak basic properties of the 4-GBA, non-aqueous titration was chosen. Glacial acetic acid was used as a solvent, and an anhydrous solution of perchloric acid in glacial acetic acid was used as a titrant. End-point detection was carried out potentiometrically (method A) and visually with violet crystalline as an indicator (method B). Using aqueous titration, we determined pKa of 4-GBA as 4.27. The developed non-aqueous titration method is of excellent linearity (r2 = 0.9995 and r2 = 0.9947 for method A and method B, respectively), accuracy (recovery from 98.8 to 101.1 % and from 98.1 to 103.2 % for method A and method B, respectively) and precision (RSD ≤ 2% for intra-and inter-day analysis). The developed method is fast, simple, cheap and is applicable for the quantitative determination of 4-GBA both in pure form, bulk and pharmaceutical formulations.

Translated title of the contributionNovel and validated non-aqueous titrimetric method for determination of perspective potassium-sparing diuretic drug candidate in pure form and pharmaceutical formulation
Original languageUndefined/Unknown
Pages (from-to)95-104
Number of pages10
JournalMalaysian Journal of Analytical Sciences
Volume25
Issue number1
Publication statusPublished - 2021

Keywords

  • Glucosides
  • Non-aqueous titration
  • Potassium-sparing diuretics
  • Quantitative determination
  • Validation methods

ASJC Scopus subject areas

  • Analytical Chemistry

Fingerprint Dive into the research topics of 'Novel and validated non-aqueous titrimetric method for determination of perspective potassium-sparing diuretic drug candidate in pure form and pharmaceutical formulation'. Together they form a unique fingerprint.

Cite this