Non-Enzymatic Electrochemistry in Characterization and Analysis of Steroid Compounds

Jan Klouda, Jiří Barek, Karel Nesměrák, Karolina Schwarzová-Pecková

Research output: Contribution to journalReview article

3 Citations (Scopus)

Abstract

This review summarizes achievements in electrochemistry-related research of steroid-based compounds in clinical, pharmaceutical, and environmental analysis. Special attention is paid to compounds possessing none or only isolated double bonds at the steroid core. Their direct redox activity is limited to far positive/negative potentials under variety of conditions and electrode materials and relies on the functional groups attached to the steroid skeleton, or as the case may be its double bond or moieties present at the side chain. The possibilities of electroanalytical methods in sterol characterization and analysis are demonstrated in a table with 31 references devoted to direct voltammetric and amperometric methods of oxidation of cholesterol, phytosterols, oxysterols, and related compounds at carbon-based and metal-based nanoparticles modified electrodes, and the reduction of bile acids and their conjugates at mercury-based electrodes, and modified glassy carbon electrodes. Furthermore, methods based on the indirect oxidation of cholesterol using bromine species as a mediator at platinum electrode and non-enzymatic cholesterol biosensors are reviewed. Their drawbacks and benefits are discussed with respect to the challenging task of identification and quantitation of these compounds in biological matrices, otherwise mostly performed using expensive mass spectrometric techniques preceded by a chromatographic separation step.

Original languageEnglish
Pages (from-to)384-404
Number of pages21
JournalCritical Reviews in Analytical Chemistry
Volume47
Issue number5
DOIs
Publication statusPublished - 3 Sep 2017
Externally publishedYes

Fingerprint

Electrochemistry
Steroids
Electrodes
Cholesterol
Phytosterols
Bromine
Oxidation
Glassy carbon
Sterols
Platinum
Bile Acids and Salts
Mercury
Biosensors
Functional groups
Carbon
Metals
Nanoparticles
Pharmaceutical Preparations

Keywords

  • Cholesterol
  • electroanalysis
  • HPLC
  • steroid hormones
  • sterols
  • voltammetry

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Non-Enzymatic Electrochemistry in Characterization and Analysis of Steroid Compounds. / Klouda, Jan; Barek, Jiří; Nesměrák, Karel; Schwarzová-Pecková, Karolina.

In: Critical Reviews in Analytical Chemistry, Vol. 47, No. 5, 03.09.2017, p. 384-404.

Research output: Contribution to journalReview article

Klouda, Jan ; Barek, Jiří ; Nesměrák, Karel ; Schwarzová-Pecková, Karolina. / Non-Enzymatic Electrochemistry in Characterization and Analysis of Steroid Compounds. In: Critical Reviews in Analytical Chemistry. 2017 ; Vol. 47, No. 5. pp. 384-404.
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