Nitric oxide metabolites induced in Anopheles stephensi control malaria parasite infection

Tina M L Peterson, Andrew J. Gow, Shirley Luckhart

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Malaria parasite infection in anopheline mosquitoes is limited by inflammatory levels of nitric oxide metabolites. To assess the mechanisms of parasite stasis or toxicity, we investigated the biochemistry of these metabolites within the blood-filled mosquito midgut. Our data indicate that nitrates, but not nitrites, are elevated in the Plasmodium-infected midgut. Although levels of S-nitrosothiols do not change with infection, blood proteins are S-nitrosylated after ingestion by the mosquito. In addition, photolyzable nitric oxide, which can be attributed to metal nitrosyls, is elevated after infection and, based on the abundance of hemoglobin, likely includes heme iron nitrosyl. The persistence of oxyhemoglobin throughout blood digestion and changes in hemoglobin conformation in response to infection suggest that hemoglobin catalyzes the synthesis of nitric oxide metabolites in a reducing environment. Provision of urate, a potent reductant and scavenger of oxidants and nitrating agents, as a dietary supplement to mosquitoes increased parasite infection levels relative to allantoin-fed controls, suggesting that nitrosative and/or oxidative stresses negatively impact developing parasites. Collectively, our results reveal a unique role for nitric oxide in an oxyhemoglobin-rich environment. In contrast to facilitating oxygen delivery by hemoglobin in the mammalian vasculature, nitric oxide synthesis in the blood-filled mosquito midgut drives the formation of toxic metabolites that limit parasite development.

Original languageEnglish
Pages (from-to)132-142
Number of pages11
JournalFree Radical Biology and Medicine
Volume42
Issue number1
DOIs
Publication statusPublished - 1 Jan 2007
Externally publishedYes

Fingerprint

Malaria control
Parasitic Diseases
Anopheles
Metabolites
Culicidae
Malaria
Nitric Oxide
Hemoglobins
Oxyhemoglobins
Parasites
Blood
Dietary supplements
Infection
S-Nitrosothiols
Allantoin
Biochemistry
Oxidative stress
Plasmodium
Poisons
Protein S

Keywords

  • Anopheles
  • Free radicals
  • Hemoglobin
  • Immunity
  • Malaria
  • Mosquito
  • Nitric oxide
  • Plasmodium

ASJC Scopus subject areas

  • Medicine(all)
  • Toxicology
  • Clinical Biochemistry

Cite this

Nitric oxide metabolites induced in Anopheles stephensi control malaria parasite infection. / Peterson, Tina M L; Gow, Andrew J.; Luckhart, Shirley.

In: Free Radical Biology and Medicine, Vol. 42, No. 1, 01.01.2007, p. 132-142.

Research output: Contribution to journalArticle

Peterson, Tina M L ; Gow, Andrew J. ; Luckhart, Shirley. / Nitric oxide metabolites induced in Anopheles stephensi control malaria parasite infection. In: Free Radical Biology and Medicine. 2007 ; Vol. 42, No. 1. pp. 132-142.
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