Abstract
Surface-enhanced Raman scattering (SERS) microspectroscopy is a very promising label-free, noncontact, and nondestructive method for real-time monitoring of extracellular matrix (ECM) development and cell integration in scaffolds for tissue engineering. Here, we prepare a new type of micrometer-sized SERS substrate, core-shell microparticles composed of solid carbonate core coated with silver nanoparticles and polyhedral multishell fullerene-like structure, astralen. Astralen has been assembled with polyallylamine hydrochloride (PAH) by the layer-by-layer manner followed by Ag nanoparticle formation by means of a silver mirror reaction, giving the final structure of composite particles CaCO3(PAH/astralen)x/Ag, where x = 1-3. The components of the microparticle carry multiple functionalities: (i) an easy identification by Raman imaging (photostable astralen) and (ii) SERS due to a rough surface of Ag nanoparticles. A combination of Ag and astralen nanoparticles provides an enhancement of astralen Raman signal by more than 1 order of magnitude. Raman signals of commonly used scaffold components such as polylactide and polyvinyl alcohol as well as ECM component (hyaluronic acid) are significantly enhanced. Thus, we demonstrate that new mechanically robust and easily detectable (by astralen signal or optically) core-shell microspheres based on biocompatible CaCO3 can be used as SERS platform. Particle design opens many future perspectives for fabrication of SERS platforms with multiple functions for biomedical applications, for example, for theranostic.
| Original language | English |
|---|---|
| Pages (from-to) | 4140-4147 |
| Number of pages | 8 |
| Journal | Langmuir |
| Volume | 29 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - 26 Mar 2013 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Electrochemistry
- Condensed Matter Physics
- Surfaces and Interfaces
- Materials Science(all)
- Spectroscopy
Cite this
New surface-enhanced raman scattering platforms : Composite calcium carbonate microspheres coated with astralen and silver nanoparticles. / Stetciura, Inna Y.; Markin, Alexey V.; Ponomarev, Andrei N.; Yakimansky, Alexander V.; Demina, Tatiana S.; Grandfils, Cristian; Volodkin, Dmitry V.; Gorin, Dmitry A.
In: Langmuir, Vol. 29, No. 12, 26.03.2013, p. 4140-4147.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - New surface-enhanced raman scattering platforms
T2 - Composite calcium carbonate microspheres coated with astralen and silver nanoparticles
AU - Stetciura, Inna Y.
AU - Markin, Alexey V.
AU - Ponomarev, Andrei N.
AU - Yakimansky, Alexander V.
AU - Demina, Tatiana S.
AU - Grandfils, Cristian
AU - Volodkin, Dmitry V.
AU - Gorin, Dmitry A.
PY - 2013/3/26
Y1 - 2013/3/26
N2 - Surface-enhanced Raman scattering (SERS) microspectroscopy is a very promising label-free, noncontact, and nondestructive method for real-time monitoring of extracellular matrix (ECM) development and cell integration in scaffolds for tissue engineering. Here, we prepare a new type of micrometer-sized SERS substrate, core-shell microparticles composed of solid carbonate core coated with silver nanoparticles and polyhedral multishell fullerene-like structure, astralen. Astralen has been assembled with polyallylamine hydrochloride (PAH) by the layer-by-layer manner followed by Ag nanoparticle formation by means of a silver mirror reaction, giving the final structure of composite particles CaCO3(PAH/astralen)x/Ag, where x = 1-3. The components of the microparticle carry multiple functionalities: (i) an easy identification by Raman imaging (photostable astralen) and (ii) SERS due to a rough surface of Ag nanoparticles. A combination of Ag and astralen nanoparticles provides an enhancement of astralen Raman signal by more than 1 order of magnitude. Raman signals of commonly used scaffold components such as polylactide and polyvinyl alcohol as well as ECM component (hyaluronic acid) are significantly enhanced. Thus, we demonstrate that new mechanically robust and easily detectable (by astralen signal or optically) core-shell microspheres based on biocompatible CaCO3 can be used as SERS platform. Particle design opens many future perspectives for fabrication of SERS platforms with multiple functions for biomedical applications, for example, for theranostic.
AB - Surface-enhanced Raman scattering (SERS) microspectroscopy is a very promising label-free, noncontact, and nondestructive method for real-time monitoring of extracellular matrix (ECM) development and cell integration in scaffolds for tissue engineering. Here, we prepare a new type of micrometer-sized SERS substrate, core-shell microparticles composed of solid carbonate core coated with silver nanoparticles and polyhedral multishell fullerene-like structure, astralen. Astralen has been assembled with polyallylamine hydrochloride (PAH) by the layer-by-layer manner followed by Ag nanoparticle formation by means of a silver mirror reaction, giving the final structure of composite particles CaCO3(PAH/astralen)x/Ag, where x = 1-3. The components of the microparticle carry multiple functionalities: (i) an easy identification by Raman imaging (photostable astralen) and (ii) SERS due to a rough surface of Ag nanoparticles. A combination of Ag and astralen nanoparticles provides an enhancement of astralen Raman signal by more than 1 order of magnitude. Raman signals of commonly used scaffold components such as polylactide and polyvinyl alcohol as well as ECM component (hyaluronic acid) are significantly enhanced. Thus, we demonstrate that new mechanically robust and easily detectable (by astralen signal or optically) core-shell microspheres based on biocompatible CaCO3 can be used as SERS platform. Particle design opens many future perspectives for fabrication of SERS platforms with multiple functions for biomedical applications, for example, for theranostic.
UR - http://www.scopus.com/inward/record.url?scp=84875618216&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84875618216&partnerID=8YFLogxK
U2 - 10.1021/la305117t
DO - 10.1021/la305117t
M3 - Article
C2 - 23470204
AN - SCOPUS:84875618216
VL - 29
SP - 4140
EP - 4147
JO - Langmuir
JF - Langmuir
SN - 0743-7463
IS - 12
ER -