New flow-through coulometric detector with renewable working electrode material for flow injection analysis and HPLC

Jan Mika, Jiri Barek, Jiri Zima, Hana Dejmkova

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    Newly developed flow-through coulometric detector with renewable working material of glassy carbon microbeads was fabricated and its basic electrochemical parameters were studied by flow injection analysis using potassium ferrocyanide and hydroquinone as probes. Main advantage of the detector is its simple construction while maintaining good electrochemical parameters and high conversion degree. Practical application of the detector was proved by the development of new HPLC and FIA method of thymol and sulfamethizole determination in pharmaceutical preparations. Optimal determination conditions were found and calibration dependences were measured. A value of quantification limit 0.97 μmol L-1 for thymol and 0.040 μmol L-1 for sulfamethizole were obtained. Newly developed methods were used for determination of the studied compounds in pharmaceutical preparations. HPLC with spectrophotometric detection was used as a comparative method; results from both methods were in good agreement.

    Original languageEnglish
    Pages (from-to)14-22
    Number of pages9
    JournalElectrochimica Acta
    Volume154
    DOIs
    Publication statusPublished - 1 Feb 2015

    Fingerprint

    Sulfamethizole
    Thymol
    Detectors
    Drug products
    Electrodes
    Glassy carbon
    Pharmaceutical Preparations
    Potassium
    Calibration

    Keywords

    • Coulometric detection
    • Glassy carbon microbeads
    • HPLC
    • Pharmaceutical preparations
    • Renewable working electrode material

    ASJC Scopus subject areas

    • Chemical Engineering(all)
    • Electrochemistry

    Cite this

    New flow-through coulometric detector with renewable working electrode material for flow injection analysis and HPLC. / Mika, Jan; Barek, Jiri; Zima, Jiri; Dejmkova, Hana.

    In: Electrochimica Acta, Vol. 154, 01.02.2015, p. 14-22.

    Research output: Contribution to journalArticle

    @article{d74aeb430e964622bd1a3997e57eca7d,
    title = "New flow-through coulometric detector with renewable working electrode material for flow injection analysis and HPLC",
    abstract = "Newly developed flow-through coulometric detector with renewable working material of glassy carbon microbeads was fabricated and its basic electrochemical parameters were studied by flow injection analysis using potassium ferrocyanide and hydroquinone as probes. Main advantage of the detector is its simple construction while maintaining good electrochemical parameters and high conversion degree. Practical application of the detector was proved by the development of new HPLC and FIA method of thymol and sulfamethizole determination in pharmaceutical preparations. Optimal determination conditions were found and calibration dependences were measured. A value of quantification limit 0.97 μmol L-1 for thymol and 0.040 μmol L-1 for sulfamethizole were obtained. Newly developed methods were used for determination of the studied compounds in pharmaceutical preparations. HPLC with spectrophotometric detection was used as a comparative method; results from both methods were in good agreement.",
    keywords = "Coulometric detection, Glassy carbon microbeads, HPLC, Pharmaceutical preparations, Renewable working electrode material",
    author = "Jan Mika and Jiri Barek and Jiri Zima and Hana Dejmkova",
    year = "2015",
    month = "2",
    day = "1",
    doi = "10.1016/j.electacta.2014.12.091",
    language = "English",
    volume = "154",
    pages = "14--22",
    journal = "Electrochimica Acta",
    issn = "0013-4686",
    publisher = "Elsevier Limited",

    }

    TY - JOUR

    T1 - New flow-through coulometric detector with renewable working electrode material for flow injection analysis and HPLC

    AU - Mika, Jan

    AU - Barek, Jiri

    AU - Zima, Jiri

    AU - Dejmkova, Hana

    PY - 2015/2/1

    Y1 - 2015/2/1

    N2 - Newly developed flow-through coulometric detector with renewable working material of glassy carbon microbeads was fabricated and its basic electrochemical parameters were studied by flow injection analysis using potassium ferrocyanide and hydroquinone as probes. Main advantage of the detector is its simple construction while maintaining good electrochemical parameters and high conversion degree. Practical application of the detector was proved by the development of new HPLC and FIA method of thymol and sulfamethizole determination in pharmaceutical preparations. Optimal determination conditions were found and calibration dependences were measured. A value of quantification limit 0.97 μmol L-1 for thymol and 0.040 μmol L-1 for sulfamethizole were obtained. Newly developed methods were used for determination of the studied compounds in pharmaceutical preparations. HPLC with spectrophotometric detection was used as a comparative method; results from both methods were in good agreement.

    AB - Newly developed flow-through coulometric detector with renewable working material of glassy carbon microbeads was fabricated and its basic electrochemical parameters were studied by flow injection analysis using potassium ferrocyanide and hydroquinone as probes. Main advantage of the detector is its simple construction while maintaining good electrochemical parameters and high conversion degree. Practical application of the detector was proved by the development of new HPLC and FIA method of thymol and sulfamethizole determination in pharmaceutical preparations. Optimal determination conditions were found and calibration dependences were measured. A value of quantification limit 0.97 μmol L-1 for thymol and 0.040 μmol L-1 for sulfamethizole were obtained. Newly developed methods were used for determination of the studied compounds in pharmaceutical preparations. HPLC with spectrophotometric detection was used as a comparative method; results from both methods were in good agreement.

    KW - Coulometric detection

    KW - Glassy carbon microbeads

    KW - HPLC

    KW - Pharmaceutical preparations

    KW - Renewable working electrode material

    UR - http://www.scopus.com/inward/record.url?scp=84919807907&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=84919807907&partnerID=8YFLogxK

    U2 - 10.1016/j.electacta.2014.12.091

    DO - 10.1016/j.electacta.2014.12.091

    M3 - Article

    AN - SCOPUS:84919807907

    VL - 154

    SP - 14

    EP - 22

    JO - Electrochimica Acta

    JF - Electrochimica Acta

    SN - 0013-4686

    ER -