New flow-through coulometric detector with renewable working electrode material for flow injection analysis and HPLC

Jan Mika, Jiri Barek, Jiri Zima, Hana Dejmkova

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    Newly developed flow-through coulometric detector with renewable working material of glassy carbon microbeads was fabricated and its basic electrochemical parameters were studied by flow injection analysis using potassium ferrocyanide and hydroquinone as probes. Main advantage of the detector is its simple construction while maintaining good electrochemical parameters and high conversion degree. Practical application of the detector was proved by the development of new HPLC and FIA method of thymol and sulfamethizole determination in pharmaceutical preparations. Optimal determination conditions were found and calibration dependences were measured. A value of quantification limit 0.97 μmol L-1 for thymol and 0.040 μmol L-1 for sulfamethizole were obtained. Newly developed methods were used for determination of the studied compounds in pharmaceutical preparations. HPLC with spectrophotometric detection was used as a comparative method; results from both methods were in good agreement.

    Original languageEnglish
    Pages (from-to)14-22
    Number of pages9
    JournalElectrochimica Acta
    Volume154
    DOIs
    Publication statusPublished - 1 Feb 2015

    Keywords

    • Coulometric detection
    • Glassy carbon microbeads
    • HPLC
    • Pharmaceutical preparations
    • Renewable working electrode material

    ASJC Scopus subject areas

    • Chemical Engineering(all)
    • Electrochemistry

    Fingerprint Dive into the research topics of 'New flow-through coulometric detector with renewable working electrode material for flow injection analysis and HPLC'. Together they form a unique fingerprint.

  • Cite this