Nano-hydroxyapatite-coated metal-ceramic composite of iron-tricalcium phosphate: Improving the surface wettability, adhesion and proliferation of mesenchymal stem cells in vitro

Maria A. Surmeneva, Claudia Kleinhans, Gabriele Vacun, Petra Juliane Kluger, Veronika Schönhaar, Michaela Müller, Sebastian Boris Hein, Alexandra Wittmar, Mathias Ulbricht, Oleg Prymak, Christian Oehr, Roman A. Surmenev

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Thin radio-frequency magnetron sputter deposited nano-hydroxyapatite (HA) films were prepared on the surface of a Fe-tricalcium phosphate (Fe-TCP) bioceramic composite, which was obtained using a conventional powder injection moulding technique. The obtained nano-hydroxyapatite coated Fe-TCP biocomposites (nano-HA-Fe-TCP) were studied with respect to their chemical and phase composition, surface morphology, water contact angle, surface free energy and hysteresis. The deposition process resulted in a homogeneous, single-phase HA coating. The ability of the surface to support adhesion and the proliferation of human mesenchymal stem cells (hMSCs) was studied using biological short-term tests in vitro. The surface of the uncoated Fe-TCP bioceramic composite showed an initial cell attachment after 24 h of seeding, but adhesion, proliferation and growth did not persist during 14 days of culture. However, the HA-Fe-TCP surfaces allowed cell adhesion, and proliferation during 14 days. The deposition of the nano-HA films on the Fe-TCP surface resulted in higher surface energy, improved hydrophilicity and biocompatibility compared with the surface of the uncoated Fe-TCP. Furthermore, it is suggested that an increase in the polar component of the surface energy was responsible for the enhanced cell adhesion and proliferation in the case of the nano-HA-Fe-TCP biocomposites.

Original languageEnglish
Pages (from-to)386-393
Number of pages8
JournalColloids and Surfaces B: Biointerfaces
Volume135
DOIs
Publication statusPublished - 1 Nov 2015

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Keywords

  • Bioceramic composite
  • Bioresorbable alloy
  • Cell adhesion
  • Hydroxyapatite coating
  • RF magnetron sputtering

ASJC Scopus subject areas

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

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