Abstract
For investigation of mumie constituents, the isolation of substances was performed by the classical method of humic substances fractionation that is based on their different solubility in water at different pH and ion-exchange properties. The elution patterns were investigated by size-exclusion chromatography using Sephadex G25, and the fractions obtained were characterized by UV-Vis absorbance, fluorescence, and infrared (IR) spectroscopy. The characteristic absorption bands typical for humic substances are observed in IR spectra of fulvic (FA), humic (HA), and hymatomelanic (HymA) acids. The chromatographic investigation has revealed the presence of two groups of characteristic fluorescent organic matters in the FA and HA fractions. The first group responsible for the long wavelength band of the fluorescence emission was assigned to FA and HA (excitation 485 nm; emission 535 nm). The second one contributing to the emission spectra in the short wavelength region (excitation 355 nm; emission 460 nm) was caused presumably by the presence of non-humic substances. Fractions enriched with the short wavelength fluorescent substances were obtained from HA and FA fractions on extraction by absolute ethanol. The organic matters in ethanolic extracts from FA is referred to as the fluorescent fraction of FA (FFFA) in the present study. We suppose that FFFA consists of coumarin derivatives, which are probably responsible for the fluorescence. FA, FFFA, and polysaccharide fractions (PFs) from mumie have been tested for their ability to modulate reactive oxygen species (ROS) production of murine peritoneal macrophages. Intracellular phorbol-12-myristate-13-acetate (PMA) stimulating the ROS formation was determined by fluorescence with the use of 2′,7′-dichlorofluorescein diacetate. The dose dependent activation in ROS production was observed with increasing concentrations of FA and PF I (fraction desorbed from DEAE cellulose by 0.5 M NaCl), and duration of cell pre-incubation with these mumie constituents. On the contrary, FFFA significantly suppressed the macrophage activity.
| Original language | English |
|---|---|
| Title of host publication | Proceedings - 6th Russian-Korean International Symposium on Science and Technology, KORUS 2002 |
| Publisher | Institute of Electrical and Electronics Engineers Inc. |
| Pages | 486-489 |
| Number of pages | 4 |
| ISBN (Electronic) | 0780374274, 9780780374270 |
| DOIs | |
| Publication status | Published - 2002 |
| Externally published | Yes |
| Event | 6th Russian-Korean International Symposium on Science and Technology, KORUS 2002 - Novosibirsk, Russian Federation Duration: 24 Jun 2002 → 30 Jun 2002 |
Conference
| Conference | 6th Russian-Korean International Symposium on Science and Technology, KORUS 2002 |
|---|---|
| Country | Russian Federation |
| City | Novosibirsk |
| Period | 24.6.02 → 30.6.02 |
Fingerprint
Keywords
- humic substances
- macrophages
- mumie
- reactive oxygen species
ASJC Scopus subject areas
- Civil and Structural Engineering
- Electrical and Electronic Engineering
- Industrial and Manufacturing Engineering
- Mechanics of Materials
- Architecture
- Electronic, Optical and Magnetic Materials
- Medicine (miscellaneous)
Cite this
Mumie constituents and their biological activity : Modulation of reactive oxygen species (ROS) production of macrophages. / Schepetkin, I. A.; Klubachuk, O. N.; Khlebnikov, Andrey Ivanovich; Kwon, B. S.
Proceedings - 6th Russian-Korean International Symposium on Science and Technology, KORUS 2002. Institute of Electrical and Electronics Engineers Inc., 2002. p. 486-489 1028071.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Mumie constituents and their biological activity
T2 - Modulation of reactive oxygen species (ROS) production of macrophages
AU - Schepetkin, I. A.
AU - Klubachuk, O. N.
AU - Khlebnikov, Andrey Ivanovich
AU - Kwon, B. S.
PY - 2002
Y1 - 2002
N2 - For investigation of mumie constituents, the isolation of substances was performed by the classical method of humic substances fractionation that is based on their different solubility in water at different pH and ion-exchange properties. The elution patterns were investigated by size-exclusion chromatography using Sephadex G25, and the fractions obtained were characterized by UV-Vis absorbance, fluorescence, and infrared (IR) spectroscopy. The characteristic absorption bands typical for humic substances are observed in IR spectra of fulvic (FA), humic (HA), and hymatomelanic (HymA) acids. The chromatographic investigation has revealed the presence of two groups of characteristic fluorescent organic matters in the FA and HA fractions. The first group responsible for the long wavelength band of the fluorescence emission was assigned to FA and HA (excitation 485 nm; emission 535 nm). The second one contributing to the emission spectra in the short wavelength region (excitation 355 nm; emission 460 nm) was caused presumably by the presence of non-humic substances. Fractions enriched with the short wavelength fluorescent substances were obtained from HA and FA fractions on extraction by absolute ethanol. The organic matters in ethanolic extracts from FA is referred to as the fluorescent fraction of FA (FFFA) in the present study. We suppose that FFFA consists of coumarin derivatives, which are probably responsible for the fluorescence. FA, FFFA, and polysaccharide fractions (PFs) from mumie have been tested for their ability to modulate reactive oxygen species (ROS) production of murine peritoneal macrophages. Intracellular phorbol-12-myristate-13-acetate (PMA) stimulating the ROS formation was determined by fluorescence with the use of 2′,7′-dichlorofluorescein diacetate. The dose dependent activation in ROS production was observed with increasing concentrations of FA and PF I (fraction desorbed from DEAE cellulose by 0.5 M NaCl), and duration of cell pre-incubation with these mumie constituents. On the contrary, FFFA significantly suppressed the macrophage activity.
AB - For investigation of mumie constituents, the isolation of substances was performed by the classical method of humic substances fractionation that is based on their different solubility in water at different pH and ion-exchange properties. The elution patterns were investigated by size-exclusion chromatography using Sephadex G25, and the fractions obtained were characterized by UV-Vis absorbance, fluorescence, and infrared (IR) spectroscopy. The characteristic absorption bands typical for humic substances are observed in IR spectra of fulvic (FA), humic (HA), and hymatomelanic (HymA) acids. The chromatographic investigation has revealed the presence of two groups of characteristic fluorescent organic matters in the FA and HA fractions. The first group responsible for the long wavelength band of the fluorescence emission was assigned to FA and HA (excitation 485 nm; emission 535 nm). The second one contributing to the emission spectra in the short wavelength region (excitation 355 nm; emission 460 nm) was caused presumably by the presence of non-humic substances. Fractions enriched with the short wavelength fluorescent substances were obtained from HA and FA fractions on extraction by absolute ethanol. The organic matters in ethanolic extracts from FA is referred to as the fluorescent fraction of FA (FFFA) in the present study. We suppose that FFFA consists of coumarin derivatives, which are probably responsible for the fluorescence. FA, FFFA, and polysaccharide fractions (PFs) from mumie have been tested for their ability to modulate reactive oxygen species (ROS) production of murine peritoneal macrophages. Intracellular phorbol-12-myristate-13-acetate (PMA) stimulating the ROS formation was determined by fluorescence with the use of 2′,7′-dichlorofluorescein diacetate. The dose dependent activation in ROS production was observed with increasing concentrations of FA and PF I (fraction desorbed from DEAE cellulose by 0.5 M NaCl), and duration of cell pre-incubation with these mumie constituents. On the contrary, FFFA significantly suppressed the macrophage activity.
KW - humic substances
KW - macrophages
KW - mumie
KW - reactive oxygen species
UR - http://www.scopus.com/inward/record.url?scp=84961575486&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84961575486&partnerID=8YFLogxK
U2 - 10.1109/KORUS.2002.1028071
DO - 10.1109/KORUS.2002.1028071
M3 - Conference contribution
AN - SCOPUS:84961575486
SP - 486
EP - 489
BT - Proceedings - 6th Russian-Korean International Symposium on Science and Technology, KORUS 2002
PB - Institute of Electrical and Electronics Engineers Inc.
ER -