TY - JOUR
T1 - Multiphoton 3D Printing of Biopolymer-Based Hydrogels
AU - Parkatzidis, Kostas
AU - Chatzinikolaidou, Maria
AU - Kaliva, Maria
AU - Bakopoulou, Athina
AU - Farsari, Maria
AU - Vamvakaki, Maria
N1 - Funding Information:
The authors would like to acknowledge Aleka Manousaki and Stefanos Papadakis for technical assistance with the SEM characterization and Dr. Margarita Stapountzi for technical assistance with laser scanning confocal microscopy. M.F. would like to acknowledge financial support from the project HELLAS-CH (MIS 5002735) implemented under “Action for Strengthening Research and Innovation Infrastructures,” funded by the Operational Programme “Competitiveness, Entrepreneurship and Innovation” (NSRF 2014-2020) and cofinanced by Greece and the European Union (European Regional Development Fund).
Publisher Copyright:
Copyright © 2019 American Chemical Society.
Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.
PY - 2019/11/11
Y1 - 2019/11/11
N2 - Multiphoton lithography, based on multiphoton polymerization, is a powerful technique for the fabrication of complex three-dimensional (3D) structures. Herein, we report on the photostructuring of novel biopolymer-based hybrid hydrogels, comprising gelatin methacrylamide and a water-soluble chitosan derivative, via multiphoton polymerization. The nontoxic, Food and Drug Administration-approved, biocompatible photosensitizer eosin Y was exploited as the sole photoinitiator, without the coinitiators and/or comonomer that are commonly used, allowing for further expansion of the available wavelengths up to 800 nm. Importantly, the obtained hybrid material exhibits excellent biocompatibility, evidenced by the increased proliferation of dental pulp stem cells, compared with the individual components and the polystyrene control, after 7 days in culture. Additionally, the 3D hybrid scaffolds promote the matrix mineralization, following their functionalization with bone morphogenetic protein 2. These tailor-made synthetic, biocompatible materials pave the way for further opportunities in 3D scaffold fabrication, including in situ and in vivo biofabrication.
AB - Multiphoton lithography, based on multiphoton polymerization, is a powerful technique for the fabrication of complex three-dimensional (3D) structures. Herein, we report on the photostructuring of novel biopolymer-based hybrid hydrogels, comprising gelatin methacrylamide and a water-soluble chitosan derivative, via multiphoton polymerization. The nontoxic, Food and Drug Administration-approved, biocompatible photosensitizer eosin Y was exploited as the sole photoinitiator, without the coinitiators and/or comonomer that are commonly used, allowing for further expansion of the available wavelengths up to 800 nm. Importantly, the obtained hybrid material exhibits excellent biocompatibility, evidenced by the increased proliferation of dental pulp stem cells, compared with the individual components and the polystyrene control, after 7 days in culture. Additionally, the 3D hybrid scaffolds promote the matrix mineralization, following their functionalization with bone morphogenetic protein 2. These tailor-made synthetic, biocompatible materials pave the way for further opportunities in 3D scaffold fabrication, including in situ and in vivo biofabrication.
KW - 3D scaffolds
KW - biomineralization
KW - BMP-2
KW - bone tissue engineering
KW - dental pulp stem cells
KW - eosin Y
KW - GelMA
KW - multiphoton lithography
KW - multiphoton polymerization
KW - water-soluble chitosan
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U2 - 10.1021/acsbiomaterials.9b01300
DO - 10.1021/acsbiomaterials.9b01300
M3 - Article
AN - SCOPUS:85074423204
VL - 5
SP - 6161
EP - 6170
JO - ACS Biomaterials Science and Engineering
JF - ACS Biomaterials Science and Engineering
SN - 2373-9878
IS - 11
ER -