Multilevel calcium phosphate coating: A model of bone-like topography for osteoimmunology

L. S. Litvinova, V. V. Malashchenko, E. O. Shunkin, V. V. Shupletsova, O. G. Khaziakhmatova, K. A. Yurova, E. S. Melashchenko, I. A. Khlusov, E. G. Komarova, V. V. Chebodaeva, Yu P. Sharkeev

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Osteoimmunolgy describes the interactions between blood immune cells and human multipotent mesenchymal stromal cells (hMMSCs) as a basis of successful wound and fracture healing. The aim was to investigate in vitro interaction of adipose-derived hMMSCs and human blood mononuclear cells (hBMNCs) modulated by calcium phosphate (CaP) coating in 3D culture. Titanium plates (10 u 10 u 1 mm3) with micro-arc bilateral multilevel CaP coatings were used. Cell-IQ phase-contrast microscopy and the real-time cell analyzer (RTCA) showed the CaP coating and hBMNCs synergistic negative effects on hMMSC motility that could be conditioned by enhanced osteogenic differentiation of stromal cells. Indeed, a 10-fold increase in the bone mineralization around the CaP-coated samples was detected in the mixed (hBMNCs + hMMSCs) 3D culture. Thus, cellular and molecular crosstalk between hBMNCs and hMMSCs modulated by multilevel micro-arc CaP coating with bone-like topography is an effective 3D model to study in vitro the novel pathways of osteoimmunology.

Original languageEnglish
Title of host publicationProceedings of the International Conference on Physical Mesomechanics. Materials with Multilevel Hierarchical Structure and Intelligent Manufacturing Technology
EditorsVictor E. Panin, Vasily M. Fomin
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735440463
DOIs
Publication statusPublished - 14 Dec 2020
EventInternational Conference on Physical Mesomechanics. Materials with Multilevel Hierarchical Structure and Intelligent Manufacturing Technology 2020 - Tomsk, Russian Federation
Duration: 5 Oct 20209 Oct 2020

Publication series

NameAIP Conference Proceedings
Volume2310
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

ConferenceInternational Conference on Physical Mesomechanics. Materials with Multilevel Hierarchical Structure and Intelligent Manufacturing Technology 2020
CountryRussian Federation
CityTomsk
Period5.10.209.10.20

ASJC Scopus subject areas

  • Physics and Astronomy(all)

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