Multifunctional Scaffolds with Improved Antimicrobial Properties and Osteogenicity Based on Piezoelectric Electrospun Fibers Decorated with Bioactive Composite Microcapsules

Alexander S. Timin, Albert R. Muslimov, Mikhail V. Zyuzin, Oleksii O. Peltek, Timofey E. Karpov, Igor S. Sergeev, Anna I. Dotsenko, Alexander A. Goncharenko, Nikita D. Yolshin, Artem Sinelnik, Bärbel Krause, Tilo Baumbach, Maria A. Surmeneva, Roman V. Chernozem, Gleb B. Sukhorukov, Roman A. Surmenev

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The incorporation of bioactive compounds onto polymer fibrous scaffolds with further control of drug release kinetics is essential to improve the functionality of scaffolds for personalized drug therapy and regenerative medicine. In this study, polymer and hybrid microcapsules were prepared and used as drug carriers, which are further deposited onto polymer microfiber scaffolds [polycaprolactone (PCL), poly(3-hydroxybutyrate) (PHB), and PHB doping with the conductive polyaniline (PANi) of 2 wt % (PHB-PANi)]. The number of immobilized microcapsules decreased with increase in their ζ-potential due to electrostatic repulsion with the negatively charged fiber surface, depending on the polymer used for the scaffold's fabrication. Additionally, the immobilization of the capsules in dynamic mechanical conditions at a frequency of 10 Hz resulted in an increase in the number of the capsules on the fibers with increase in the scaffold piezoelectric response in the order PCL < PHB < PHB-PANi, depending on the chemical composition of the capsules. The immobilization of microcapsules loaded with different bioactive molecules onto the scaffold surface enabled multimodal triggering by physical (ultrasound, laser radiation) and biological (enzymatic treatment) stimuli, providing controllable release of the cargo from scaffolds. Importantly, the microcapsules immobilized onto the surface of the scaffolds did not influence the cell growth, viability, and cell proliferation on the scaffolds. Moreover, the attachment of human mesenchymal stem cells (hMSCs) on the scaffolds revealed that the PHB and PHB-PANi scaffolds promoted adhesion of hMSCs compared to that of the PCL scaffolds. Two bioactive compounds, antibiotic ceftriaxone sodium (CS) and osteogenic factor dexamethasone (DEXA), were chosen to load the microcapsules and demonstrate the antimicrobial properties and osteogenesis of the scaffolds. The modified scaffolds had prolonged release of CS or DEXA, which provided an improved antimicrobial effect, as well as enhanced osteogenic differentiation and mineralization of the scaffolds modified with capsules compared to that of individual scaffolds soaked in CS solution or incubated in an osteogenic medium. Thus, the immobilization of microcapsules provides a simple, convenient way to incorporate bioactive compounds onto polymer scaffolds, which makes these multimodal materials suitable for personalized drug therapy and bone tissue engineering.

Original languageEnglish
Pages (from-to)34849-34868
Number of pages20
JournalACS Applied Materials and Interfaces
Volume10
Issue number41
DOIs
Publication statusPublished - 17 Oct 2018

Fingerprint

Scaffolds
Capsules
Fibers
Composite materials
Polyaniline
Polymers
Polycaprolactone
Ceftriaxone
Drug therapy
Sodium
Scaffolds (biology)
Stem cells
Dexamethasone
Drug Carriers
Cell proliferation
Cell growth
Antibiotics
Laser radiation
poly-beta-hydroxybutyrate
Tissue engineering

Keywords

  • antibacterial properties
  • cell adhesion
  • osteogenic differentiation
  • polyelectrolyte and hybrid microcapsules
  • polymer scaffolds
  • sol-gel coating

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Multifunctional Scaffolds with Improved Antimicrobial Properties and Osteogenicity Based on Piezoelectric Electrospun Fibers Decorated with Bioactive Composite Microcapsules. / Timin, Alexander S.; Muslimov, Albert R.; Zyuzin, Mikhail V.; Peltek, Oleksii O.; Karpov, Timofey E.; Sergeev, Igor S.; Dotsenko, Anna I.; Goncharenko, Alexander A.; Yolshin, Nikita D.; Sinelnik, Artem; Krause, Bärbel; Baumbach, Tilo; Surmeneva, Maria A.; Chernozem, Roman V.; Sukhorukov, Gleb B.; Surmenev, Roman A.

In: ACS Applied Materials and Interfaces, Vol. 10, No. 41, 17.10.2018, p. 34849-34868.

Research output: Contribution to journalArticle

Timin, AS, Muslimov, AR, Zyuzin, MV, Peltek, OO, Karpov, TE, Sergeev, IS, Dotsenko, AI, Goncharenko, AA, Yolshin, ND, Sinelnik, A, Krause, B, Baumbach, T, Surmeneva, MA, Chernozem, RV, Sukhorukov, GB & Surmenev, RA 2018, 'Multifunctional Scaffolds with Improved Antimicrobial Properties and Osteogenicity Based on Piezoelectric Electrospun Fibers Decorated with Bioactive Composite Microcapsules', ACS Applied Materials and Interfaces, vol. 10, no. 41, pp. 34849-34868. https://doi.org/10.1021/acsami.8b09810
Timin, Alexander S. ; Muslimov, Albert R. ; Zyuzin, Mikhail V. ; Peltek, Oleksii O. ; Karpov, Timofey E. ; Sergeev, Igor S. ; Dotsenko, Anna I. ; Goncharenko, Alexander A. ; Yolshin, Nikita D. ; Sinelnik, Artem ; Krause, Bärbel ; Baumbach, Tilo ; Surmeneva, Maria A. ; Chernozem, Roman V. ; Sukhorukov, Gleb B. ; Surmenev, Roman A. / Multifunctional Scaffolds with Improved Antimicrobial Properties and Osteogenicity Based on Piezoelectric Electrospun Fibers Decorated with Bioactive Composite Microcapsules. In: ACS Applied Materials and Interfaces. 2018 ; Vol. 10, No. 41. pp. 34849-34868.
@article{a32c31a19a9b47288c746cbdcf3b0eef,
title = "Multifunctional Scaffolds with Improved Antimicrobial Properties and Osteogenicity Based on Piezoelectric Electrospun Fibers Decorated with Bioactive Composite Microcapsules",
abstract = "The incorporation of bioactive compounds onto polymer fibrous scaffolds with further control of drug release kinetics is essential to improve the functionality of scaffolds for personalized drug therapy and regenerative medicine. In this study, polymer and hybrid microcapsules were prepared and used as drug carriers, which are further deposited onto polymer microfiber scaffolds [polycaprolactone (PCL), poly(3-hydroxybutyrate) (PHB), and PHB doping with the conductive polyaniline (PANi) of 2 wt {\%} (PHB-PANi)]. The number of immobilized microcapsules decreased with increase in their ζ-potential due to electrostatic repulsion with the negatively charged fiber surface, depending on the polymer used for the scaffold's fabrication. Additionally, the immobilization of the capsules in dynamic mechanical conditions at a frequency of 10 Hz resulted in an increase in the number of the capsules on the fibers with increase in the scaffold piezoelectric response in the order PCL < PHB < PHB-PANi, depending on the chemical composition of the capsules. The immobilization of microcapsules loaded with different bioactive molecules onto the scaffold surface enabled multimodal triggering by physical (ultrasound, laser radiation) and biological (enzymatic treatment) stimuli, providing controllable release of the cargo from scaffolds. Importantly, the microcapsules immobilized onto the surface of the scaffolds did not influence the cell growth, viability, and cell proliferation on the scaffolds. Moreover, the attachment of human mesenchymal stem cells (hMSCs) on the scaffolds revealed that the PHB and PHB-PANi scaffolds promoted adhesion of hMSCs compared to that of the PCL scaffolds. Two bioactive compounds, antibiotic ceftriaxone sodium (CS) and osteogenic factor dexamethasone (DEXA), were chosen to load the microcapsules and demonstrate the antimicrobial properties and osteogenesis of the scaffolds. The modified scaffolds had prolonged release of CS or DEXA, which provided an improved antimicrobial effect, as well as enhanced osteogenic differentiation and mineralization of the scaffolds modified with capsules compared to that of individual scaffolds soaked in CS solution or incubated in an osteogenic medium. Thus, the immobilization of microcapsules provides a simple, convenient way to incorporate bioactive compounds onto polymer scaffolds, which makes these multimodal materials suitable for personalized drug therapy and bone tissue engineering.",
keywords = "antibacterial properties, cell adhesion, osteogenic differentiation, polyelectrolyte and hybrid microcapsules, polymer scaffolds, sol-gel coating",
author = "Timin, {Alexander S.} and Muslimov, {Albert R.} and Zyuzin, {Mikhail V.} and Peltek, {Oleksii O.} and Karpov, {Timofey E.} and Sergeev, {Igor S.} and Dotsenko, {Anna I.} and Goncharenko, {Alexander A.} and Yolshin, {Nikita D.} and Artem Sinelnik and B{\"a}rbel Krause and Tilo Baumbach and Surmeneva, {Maria A.} and Chernozem, {Roman V.} and Sukhorukov, {Gleb B.} and Surmenev, {Roman A.}",
year = "2018",
month = "10",
day = "17",
doi = "10.1021/acsami.8b09810",
language = "English",
volume = "10",
pages = "34849--34868",
journal = "ACS applied materials & interfaces",
issn = "1944-8244",
publisher = "American Chemical Society",
number = "41",

}

TY - JOUR

T1 - Multifunctional Scaffolds with Improved Antimicrobial Properties and Osteogenicity Based on Piezoelectric Electrospun Fibers Decorated with Bioactive Composite Microcapsules

AU - Timin, Alexander S.

AU - Muslimov, Albert R.

AU - Zyuzin, Mikhail V.

AU - Peltek, Oleksii O.

AU - Karpov, Timofey E.

AU - Sergeev, Igor S.

AU - Dotsenko, Anna I.

AU - Goncharenko, Alexander A.

AU - Yolshin, Nikita D.

AU - Sinelnik, Artem

AU - Krause, Bärbel

AU - Baumbach, Tilo

AU - Surmeneva, Maria A.

AU - Chernozem, Roman V.

AU - Sukhorukov, Gleb B.

AU - Surmenev, Roman A.

PY - 2018/10/17

Y1 - 2018/10/17

N2 - The incorporation of bioactive compounds onto polymer fibrous scaffolds with further control of drug release kinetics is essential to improve the functionality of scaffolds for personalized drug therapy and regenerative medicine. In this study, polymer and hybrid microcapsules were prepared and used as drug carriers, which are further deposited onto polymer microfiber scaffolds [polycaprolactone (PCL), poly(3-hydroxybutyrate) (PHB), and PHB doping with the conductive polyaniline (PANi) of 2 wt % (PHB-PANi)]. The number of immobilized microcapsules decreased with increase in their ζ-potential due to electrostatic repulsion with the negatively charged fiber surface, depending on the polymer used for the scaffold's fabrication. Additionally, the immobilization of the capsules in dynamic mechanical conditions at a frequency of 10 Hz resulted in an increase in the number of the capsules on the fibers with increase in the scaffold piezoelectric response in the order PCL < PHB < PHB-PANi, depending on the chemical composition of the capsules. The immobilization of microcapsules loaded with different bioactive molecules onto the scaffold surface enabled multimodal triggering by physical (ultrasound, laser radiation) and biological (enzymatic treatment) stimuli, providing controllable release of the cargo from scaffolds. Importantly, the microcapsules immobilized onto the surface of the scaffolds did not influence the cell growth, viability, and cell proliferation on the scaffolds. Moreover, the attachment of human mesenchymal stem cells (hMSCs) on the scaffolds revealed that the PHB and PHB-PANi scaffolds promoted adhesion of hMSCs compared to that of the PCL scaffolds. Two bioactive compounds, antibiotic ceftriaxone sodium (CS) and osteogenic factor dexamethasone (DEXA), were chosen to load the microcapsules and demonstrate the antimicrobial properties and osteogenesis of the scaffolds. The modified scaffolds had prolonged release of CS or DEXA, which provided an improved antimicrobial effect, as well as enhanced osteogenic differentiation and mineralization of the scaffolds modified with capsules compared to that of individual scaffolds soaked in CS solution or incubated in an osteogenic medium. Thus, the immobilization of microcapsules provides a simple, convenient way to incorporate bioactive compounds onto polymer scaffolds, which makes these multimodal materials suitable for personalized drug therapy and bone tissue engineering.

AB - The incorporation of bioactive compounds onto polymer fibrous scaffolds with further control of drug release kinetics is essential to improve the functionality of scaffolds for personalized drug therapy and regenerative medicine. In this study, polymer and hybrid microcapsules were prepared and used as drug carriers, which are further deposited onto polymer microfiber scaffolds [polycaprolactone (PCL), poly(3-hydroxybutyrate) (PHB), and PHB doping with the conductive polyaniline (PANi) of 2 wt % (PHB-PANi)]. The number of immobilized microcapsules decreased with increase in their ζ-potential due to electrostatic repulsion with the negatively charged fiber surface, depending on the polymer used for the scaffold's fabrication. Additionally, the immobilization of the capsules in dynamic mechanical conditions at a frequency of 10 Hz resulted in an increase in the number of the capsules on the fibers with increase in the scaffold piezoelectric response in the order PCL < PHB < PHB-PANi, depending on the chemical composition of the capsules. The immobilization of microcapsules loaded with different bioactive molecules onto the scaffold surface enabled multimodal triggering by physical (ultrasound, laser radiation) and biological (enzymatic treatment) stimuli, providing controllable release of the cargo from scaffolds. Importantly, the microcapsules immobilized onto the surface of the scaffolds did not influence the cell growth, viability, and cell proliferation on the scaffolds. Moreover, the attachment of human mesenchymal stem cells (hMSCs) on the scaffolds revealed that the PHB and PHB-PANi scaffolds promoted adhesion of hMSCs compared to that of the PCL scaffolds. Two bioactive compounds, antibiotic ceftriaxone sodium (CS) and osteogenic factor dexamethasone (DEXA), were chosen to load the microcapsules and demonstrate the antimicrobial properties and osteogenesis of the scaffolds. The modified scaffolds had prolonged release of CS or DEXA, which provided an improved antimicrobial effect, as well as enhanced osteogenic differentiation and mineralization of the scaffolds modified with capsules compared to that of individual scaffolds soaked in CS solution or incubated in an osteogenic medium. Thus, the immobilization of microcapsules provides a simple, convenient way to incorporate bioactive compounds onto polymer scaffolds, which makes these multimodal materials suitable for personalized drug therapy and bone tissue engineering.

KW - antibacterial properties

KW - cell adhesion

KW - osteogenic differentiation

KW - polyelectrolyte and hybrid microcapsules

KW - polymer scaffolds

KW - sol-gel coating

UR - http://www.scopus.com/inward/record.url?scp=85054606623&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85054606623&partnerID=8YFLogxK

U2 - 10.1021/acsami.8b09810

DO - 10.1021/acsami.8b09810

M3 - Article

VL - 10

SP - 34849

EP - 34868

JO - ACS applied materials & interfaces

JF - ACS applied materials & interfaces

SN - 1944-8244

IS - 41

ER -