Multi-scale, multi-modal fusion of histological and MRI volumes for characterization of lung inammation

Mirabela Rusu, Haibo Wang, Thea Golden, Andrew Gow, Anant Madabhushi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Mouse lung models facilitate the investigation of conditions such as chronic inammation which are associate with common lung diseases. The multi-scale manifestation of lung inammation prompted us to use multi-scale imaging - both in vivo , ex vivo MRI along with ex vivo histology, for its study in a new quantitative way. Some imaging modalities, such as MRI, are non-invasive and capture macroscopic features of the pathology, while others, e.g. ex vivo histology, depict detailed structures. Registering such multi-modal data to the same spatial coordinates will allow the construction of a comprehensive 3D model to enable the multi-scale study of diseases. Moreover, it may facilitate the identification and definition of quantitative of in vivo imaging signatures for diseases and pathologic processes. We introduce a quantitative, image analytic framework to integrate in vivo MR images of the entire mouse with ex vivo histology of the lung alone, using lung ex vivo MRI as conduit to facilitate their co-registration. In our framework, we first align the MR images by registering the in vivo and ex vivo MRI of the lung using an interactive rigid registration approach. Then we reconstruct the 3D volume of the ex vivo histological specimen by eficient groupwise registration of the 2D slices. The resulting 3D histologic volume is subsequently registered to the MRI volumes by interactive rigid registration, directly to the ex vivo MRI, and implicitly to in vivo MRI. Qualitative evaluation of the registration framework was performed by comparing airway tree structures in ex vivo MRI and ex vivo histology where airways are visible and may be annotated. We present a use case for evaluation of our co-registration framework in the context of studying chronic inammation in a diseased mouse.

Original languageEnglish
Title of host publicationMedical Imaging 2013: Biomedical Applications in Molecular, Structural, and Functional Imaging
Volume8672
DOIs
Publication statusPublished - 2013
Externally publishedYes
EventMedical Imaging 2013: Biomedical Applications in Molecular, Structural, and Functional Imaging - Lake Buena Vista, FL, United States
Duration: 10 Feb 201313 Feb 2013

Conference

ConferenceMedical Imaging 2013: Biomedical Applications in Molecular, Structural, and Functional Imaging
CountryUnited States
CityLake Buena Vista, FL
Period10.2.1313.2.13

Fingerprint

Magnetic resonance imaging
lungs
Fusion reactions
histology
fusion
Histology
Lung
mice
Imaging techniques
Pathologic Processes
evaluation
Lung Diseases
pathology
Pulmonary diseases
Pathology
signatures

Keywords

  • Branchi
  • Chronic
  • Ex vivo mri
  • Histopathology
  • In vivo mri
  • Inammation
  • Mouse lung model

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Rusu, M., Wang, H., Golden, T., Gow, A., & Madabhushi, A. (2013). Multi-scale, multi-modal fusion of histological and MRI volumes for characterization of lung inammation. In Medical Imaging 2013: Biomedical Applications in Molecular, Structural, and Functional Imaging (Vol. 8672). [86720X] https://doi.org/10.1117/12.2007148

Multi-scale, multi-modal fusion of histological and MRI volumes for characterization of lung inammation. / Rusu, Mirabela; Wang, Haibo; Golden, Thea; Gow, Andrew; Madabhushi, Anant.

Medical Imaging 2013: Biomedical Applications in Molecular, Structural, and Functional Imaging. Vol. 8672 2013. 86720X.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Rusu, M, Wang, H, Golden, T, Gow, A & Madabhushi, A 2013, Multi-scale, multi-modal fusion of histological and MRI volumes for characterization of lung inammation. in Medical Imaging 2013: Biomedical Applications in Molecular, Structural, and Functional Imaging. vol. 8672, 86720X, Medical Imaging 2013: Biomedical Applications in Molecular, Structural, and Functional Imaging, Lake Buena Vista, FL, United States, 10.2.13. https://doi.org/10.1117/12.2007148
Rusu M, Wang H, Golden T, Gow A, Madabhushi A. Multi-scale, multi-modal fusion of histological and MRI volumes for characterization of lung inammation. In Medical Imaging 2013: Biomedical Applications in Molecular, Structural, and Functional Imaging. Vol. 8672. 2013. 86720X https://doi.org/10.1117/12.2007148
Rusu, Mirabela ; Wang, Haibo ; Golden, Thea ; Gow, Andrew ; Madabhushi, Anant. / Multi-scale, multi-modal fusion of histological and MRI volumes for characterization of lung inammation. Medical Imaging 2013: Biomedical Applications in Molecular, Structural, and Functional Imaging. Vol. 8672 2013.
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