Morphology alterations of skin and subcutaneous fat at NIR laser irradiation combined with delivery of encapsulated indocyanine green

Irina Yu Yanina, Nikita A. Navolokin, Yulia I. Svenskaya, Alla B. Bucharskaya, Galina N. Maslyakova, Dmitry A. Gorin, Gleb B. Sukhorukov, Valery V. Tuchin

Research output: Contribution to journalArticle

Abstract

The goal of this study is to quantify the impact of the in vivo photochemical treatment of rats with obesity using indocyanine green (ICG) dissolved in saline or dispersed in an encapsulated form at NIR laser irradiation, which was monitored by tissue sampling and histochemistry. The subcutaneous injection of the ICG solution or ICG encapsulated into polyelectrolyte microcapsules, followed by diode laser irradiation (808 nm, 8 W/cm2, 1 min), resulted in substantial differences in lipolysis of subcutaneous fat. Most of the morphology alterations occurred in response to the laser irradiation if a free-ICG solution had been injected. In such conditions, membrane disruption, stretching, and even delamination in some cases were observed for a number of cells. The encapsulated ICG aroused similar morphology changes but with weakly expressed adipocyte destruction under the laser irradiation. The Cochran Q test rendered the difference between the treatment alternatives statistically significant. By this means, laser treatment using the encapsulated form of ICG seems more promising and could be used for safe layerwise laser treatment of obesity and cellulite.

Original languageEnglish
Article number055008
JournalJournal of Biomedical Optics
Volume22
Issue number5
DOIs
Publication statusPublished - 2017
Externally publishedYes

Fingerprint

Indocyanine Green
fats
Laser beam effects
Oils and fats
delivery
Skin
Fats
irradiation
obesity
lasers
Lasers
Polyelectrolytes
Delamination
Stretching
Semiconductor lasers
Rats
rats
destruction
Tissue
Sampling

Keywords

  • Adipose tissue
  • Cell lipolysis
  • Cellulite
  • Histological analysis
  • Indocyanine green
  • Layer by layer assembly
  • NIR laser treatment
  • Obesity
  • Photochemical action
  • Polyelectrolyte microcapsules

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Biomedical Engineering

Cite this

Yanina, I. Y., Navolokin, N. A., Svenskaya, Y. I., Bucharskaya, A. B., Maslyakova, G. N., Gorin, D. A., ... Tuchin, V. V. (2017). Morphology alterations of skin and subcutaneous fat at NIR laser irradiation combined with delivery of encapsulated indocyanine green. Journal of Biomedical Optics, 22(5), [055008]. https://doi.org/10.1117/1.JBO.22.5.055008

Morphology alterations of skin and subcutaneous fat at NIR laser irradiation combined with delivery of encapsulated indocyanine green. / Yanina, Irina Yu; Navolokin, Nikita A.; Svenskaya, Yulia I.; Bucharskaya, Alla B.; Maslyakova, Galina N.; Gorin, Dmitry A.; Sukhorukov, Gleb B.; Tuchin, Valery V.

In: Journal of Biomedical Optics, Vol. 22, No. 5, 055008, 2017.

Research output: Contribution to journalArticle

Yanina, Irina Yu ; Navolokin, Nikita A. ; Svenskaya, Yulia I. ; Bucharskaya, Alla B. ; Maslyakova, Galina N. ; Gorin, Dmitry A. ; Sukhorukov, Gleb B. ; Tuchin, Valery V. / Morphology alterations of skin and subcutaneous fat at NIR laser irradiation combined with delivery of encapsulated indocyanine green. In: Journal of Biomedical Optics. 2017 ; Vol. 22, No. 5.
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