Morphology alterations of skin and subcutaneous fat at NIR laser irradiation combined with delivery of encapsulated indocyanine green

Irina Yu Yanina, Nikita A. Navolokin, Yulia I. Svenskaya, Alla B. Bucharskaya, Galina N. Maslyakova, Dmitry A. Gorin, Gleb B. Sukhorukov, Valery V. Tuchin

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1 Citation (Scopus)

Abstract

The goal of this study is to quantify the impact of the in vivo photochemical treatment of rats with obesity using indocyanine green (ICG) dissolved in saline or dispersed in an encapsulated form at NIR laser irradiation, which was monitored by tissue sampling and histochemistry. The subcutaneous injection of the ICG solution or ICG encapsulated into polyelectrolyte microcapsules, followed by diode laser irradiation (808 nm, 8 W/cm2, 1 min), resulted in substantial differences in lipolysis of subcutaneous fat. Most of the morphology alterations occurred in response to the laser irradiation if a free-ICG solution had been injected. In such conditions, membrane disruption, stretching, and even delamination in some cases were observed for a number of cells. The encapsulated ICG aroused similar morphology changes but with weakly expressed adipocyte destruction under the laser irradiation. The Cochran Q test rendered the difference between the treatment alternatives statistically significant. By this means, laser treatment using the encapsulated form of ICG seems more promising and could be used for safe layerwise laser treatment of obesity and cellulite.

Original languageEnglish
Article number055008
JournalJournal of Biomedical Optics
Volume22
Issue number5
DOIs
Publication statusPublished - 2017
Externally publishedYes

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Keywords

  • Adipose tissue
  • Cell lipolysis
  • Cellulite
  • Histological analysis
  • Indocyanine green
  • Layer by layer assembly
  • NIR laser treatment
  • Obesity
  • Photochemical action
  • Polyelectrolyte microcapsules

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Biomedical Engineering

Cite this

Yanina, I. Y., Navolokin, N. A., Svenskaya, Y. I., Bucharskaya, A. B., Maslyakova, G. N., Gorin, D. A., Sukhorukov, G. B., & Tuchin, V. V. (2017). Morphology alterations of skin and subcutaneous fat at NIR laser irradiation combined with delivery of encapsulated indocyanine green. Journal of Biomedical Optics, 22(5), [055008]. https://doi.org/10.1117/1.JBO.22.5.055008