Morphological changes of the red blood cells treated with metal oxide nanoparticles

A. I. Kozelskaya, A. V. Panin, I. A. Khlusov, P. V. Mokrushnikov, B. N. Zaitsev, D. I. Kuzmenko, G. Yu Vasyukov

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The toxic effect of Al2O3, SiО2 and ZrО2 nanoparticles on red blood cells of Wistar rats was studied in vitro using the atomic force microscopy and the fluorescence analysis. Transformation of discocytes into echinocytes and spherocytes caused by the metal oxide nanoparticles was revealed. It was shown that only extremely high concentration of the nanoparticles (2 mg/ml) allows correct estimating of their effect on the cell morphology. Besides, it was found out that the microviscosity changes of red blood cell membranes treated with nanoparticles began long before morphological modifications of the cells. On the contrary, the negatively charged ZrO2 and SiO2 nanoparticles did not affect ghost microviscosity up to concentrations of 1 μg/ml and 0.1 mg/ml, correspondingly. In its turn, the positively charged Al2O3 nanoparticles induced structural changes in the lipid bilayer of the red blood cells already at a concentration of 0.05 μg/ml. A decrease in microviscosity of the erythrocyte ghosts treated with Al2O3 and SiO2 nanoparticles was shown. It was detected that the interaction of ZrO2 nanoparticles with the cells led to an increase in the membrane microviscosity and cracking of swollen erythrocytes.

Original languageEnglish
Pages (from-to)34-40
Number of pages7
JournalToxicology in Vitro
Volume37
DOIs
Publication statusPublished - 1 Dec 2016

Fingerprint

Metal Nanoparticles
Nanoparticles
Oxides
Blood
Erythrocytes
Metals
Cells
Spherocytes
Atomic Force Microscopy
Poisons
Erythrocyte Membrane
Lipid Bilayers
Lipid bilayers
Cell membranes
Wistar Rats
Fluorescence
Rats
Atomic force microscopy
Cell Membrane
Membranes

Keywords

  • AFM
  • Fluorescence analysis
  • In vitro
  • Membrane
  • Metal oxide nanoparticle
  • Red blood cells

ASJC Scopus subject areas

  • Toxicology

Cite this

Morphological changes of the red blood cells treated with metal oxide nanoparticles. / Kozelskaya, A. I.; Panin, A. V.; Khlusov, I. A.; Mokrushnikov, P. V.; Zaitsev, B. N.; Kuzmenko, D. I.; Vasyukov, G. Yu.

In: Toxicology in Vitro, Vol. 37, 01.12.2016, p. 34-40.

Research output: Contribution to journalArticle

Kozelskaya, A. I. ; Panin, A. V. ; Khlusov, I. A. ; Mokrushnikov, P. V. ; Zaitsev, B. N. ; Kuzmenko, D. I. ; Vasyukov, G. Yu. / Morphological changes of the red blood cells treated with metal oxide nanoparticles. In: Toxicology in Vitro. 2016 ; Vol. 37. pp. 34-40.
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