Molecular mechanism of AHSP-mediated stabilization of α-hemoglobin

Liang Feng, David A. Gell, Suiping Zhou, Lichuan Gu, Yi Kong, Jianqing Li, Min Hu, Nieng Yan, Christopher Lee, Anne M. Rich, Robert S. Armstrong, Peter A. Lay, Andrew J. Gow, Mitchell J. Weiss, Joel P. MacKay, Yigong Shi

Research output: Contribution to journalArticle

113 Citations (Scopus)

Abstract

Hemoglobin A (HbA), the oxygen delivery system in humans, comprises two α and two β subunits. Free α-hemoglobin (αHb) is unstable, and its precipitation contributes to the pathophysiology of β thalassemia. In erythrocytes, the α-hemoglobin stabilizing protein (AHSP) binds αHb and inhibits its precipitation. The crystal structure of AHSP bound to Fe(II)-αHb reveals that AHSP specifically recognizes the G and H helices of αHb through a hydrophobic interface that largely recapitulates the α11 interface of hemoglobin. The AHSP-αHb interactions are extensive but suboptimal, explaining why β-hemoglobin can competitively displace AHSP to form HbA. Remarkably, the Fe(II)-heme group in AHSP bound αHb is coordinated by the distal but not the proximal histidine. Importantly, binding to AHSP facilitates the conversion of oxy-αHb to a deoxygenated, oxidized [Fe(III)], nonreactive form in which all six coordinate positions are occupied. These observations reveal the molecular mechanisms by which AHSP stabilizes free αHb.

Original languageEnglish
Pages (from-to)629-640
Number of pages12
JournalCell
Volume119
Issue number5
DOIs
Publication statusPublished - 24 Nov 2004
Externally publishedYes

Fingerprint

Hemoglobins
Stabilization
Hemoglobin A
Hemoglobin H
Thalassemia
Heme
Histidine
Erythrocytes
Crystal structure
Oxygen

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Feng, L., Gell, D. A., Zhou, S., Gu, L., Kong, Y., Li, J., ... Shi, Y. (2004). Molecular mechanism of AHSP-mediated stabilization of α-hemoglobin. Cell, 119(5), 629-640. https://doi.org/10.1016/j.cell.2004.11.025

Molecular mechanism of AHSP-mediated stabilization of α-hemoglobin. / Feng, Liang; Gell, David A.; Zhou, Suiping; Gu, Lichuan; Kong, Yi; Li, Jianqing; Hu, Min; Yan, Nieng; Lee, Christopher; Rich, Anne M.; Armstrong, Robert S.; Lay, Peter A.; Gow, Andrew J.; Weiss, Mitchell J.; MacKay, Joel P.; Shi, Yigong.

In: Cell, Vol. 119, No. 5, 24.11.2004, p. 629-640.

Research output: Contribution to journalArticle

Feng, L, Gell, DA, Zhou, S, Gu, L, Kong, Y, Li, J, Hu, M, Yan, N, Lee, C, Rich, AM, Armstrong, RS, Lay, PA, Gow, AJ, Weiss, MJ, MacKay, JP & Shi, Y 2004, 'Molecular mechanism of AHSP-mediated stabilization of α-hemoglobin', Cell, vol. 119, no. 5, pp. 629-640. https://doi.org/10.1016/j.cell.2004.11.025
Feng L, Gell DA, Zhou S, Gu L, Kong Y, Li J et al. Molecular mechanism of AHSP-mediated stabilization of α-hemoglobin. Cell. 2004 Nov 24;119(5):629-640. https://doi.org/10.1016/j.cell.2004.11.025
Feng, Liang ; Gell, David A. ; Zhou, Suiping ; Gu, Lichuan ; Kong, Yi ; Li, Jianqing ; Hu, Min ; Yan, Nieng ; Lee, Christopher ; Rich, Anne M. ; Armstrong, Robert S. ; Lay, Peter A. ; Gow, Andrew J. ; Weiss, Mitchell J. ; MacKay, Joel P. ; Shi, Yigong. / Molecular mechanism of AHSP-mediated stabilization of α-hemoglobin. In: Cell. 2004 ; Vol. 119, No. 5. pp. 629-640.
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