Molekuliarnaia identifikatsiia i kharakteristika hRPC11, samoi maloi spetsificheskoi sub''edinitsy RNK-polimerazy III cheloveka.

Translated title of the contribution: Molecular identification and characteristics of hRPC11, the smallest specific subunit of human RNA polymerase III

G. V. Spakovskii, E. N. Lebedenko

Research output: Contribution to journalLetterpeer-review

10 Citations (Scopus)

Abstract

Full-length copies of cDNA of the hRPC11 gene encoding the smallest specific subunit of nuclear RNA polymerase III were identified among human transcripts with the use of the RT-PCR technique. The cloning of the first orthologue of the subunit RPC11 from a multicellular organism and the comparison of subunit hRPC11 of Homo sapiens (108 aa; M(r), 12.3 kDa; pI 8.05) deduced from the cDNA primary structure with the homologous components of RNA polymerase III from Saccharomyces cerevisiae and Schizosaccharomyces pombe revealed the most important functional domains: a Zn-binding motif of the classic type (CxxCx16-17CxxC) at the N-terminal region, and two extended regions of homology (KEVDDVLGG and RSADEPM) in the central and C-terminal parts of the molecule, respectively. The C-terminus of the RPC11 subunits is highly homologous to the unique zinc ribbon of the elongation factor TFIIS, which suggests a role for this subunit in the elongation or termination of RNA synthesis.

Translated title of the contributionMolecular identification and characteristics of hRPC11, the smallest specific subunit of human RNA polymerase III
Original languageRussian
Pages (from-to)877-880
Number of pages4
JournalBioorganicheskaia khimiia
Volume24
Issue number11
Publication statusPublished - Nov 1998
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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