Molecular cloning of some components of the translation apparatus of fission yeast Schizosaccharomyces pombe and a unified list of genes of its cytoplasmic ribosomal proteins

G. V. Shpakovski, G. M. Baranova, V. Wood, R. G. Gwilliam, E. K. Shematorova, O. L. Korol'chuk, E. N. Lebedenko

Research output: Contribution to journalArticlepeer-review

Abstract

Full-length cDNAs of four new genes encoding cytoplasmic ribosomal proteins L14 and L20 (large ribosomal subunit) and S1 and S27 (small ribosomal subunit) were isolated and sequenced during the analysis of the fission yeast Schizosaccharomyces pombe genome. One of the Sz. pombe genes encoding translation elongation factor EF-2 was also cloned, and its precise position on chromosome I was established. A unified nomenclature was proposed, and the list of all known genetic determinants encoding cytoplasmic ribosomal proteins of Sz. pombe was compiled. By now, 76 genes/cDNAs encoding different ribosomal proteins have been identified in the fission yeast genome. Among them, 34 genes are duplicated, and three homologous genes are identified for each of the ribosomal proteins L2, L16, P1, and P2.

Original languageEnglish
Pages (from-to)397-409
Number of pages13
JournalRussian Journal of Bioorganic Chemistry
Volume25
Issue number6
Publication statusPublished - Jun 1999
Externally publishedYes

Keywords

  • cDNAs and genes for proteins L14 (rpl14), L20 (rpl20), S1 (rps1), and S27 (rps27)
  • Cytoplasmic ribosomal proteins list and unified nomenclature
  • Genome analysis
  • Translation elongation factor EF-2 (eft2a gene)
  • Yeast schizosaccharomyces pombe

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry

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