TY - JOUR
T1 - Molecular Cloning and Characterization of cDNA of the rpc10+ Gene Encoding the Smallest Subunit of Nuclear RNA Polymerases of Schizosaccharomyces pombe
AU - Shpakovski, G. V.
AU - Lebedenko, E. N.
N1 - Copyright:
Copyright 2004 Elsevier Science B.V., Amsterdam. All rights reserved.
PY - 1997
Y1 - 1997
N2 - The full-length cDNA of the rpc10+ gene encoding mini-subunit Rpc10, which is common for all three nuclear RNA polymerases of the fission yeast Schizosaccharomyces pombe, was cloned and sequenced. The Rpc10 subunit of Sz. pombe and its homologs from S. cerevisiae and H. sapiens are positively charged proteins with a highly conserved C-terminal region and an invariant zinc-binding domain (Zn-finger) of a typical amino acid composition: YxCx2Cx12RCx2CGxR. Functional tests on heterospecific complementation, using tetrad analysis or plasmid shuffling, showed that the Rpc10 subunit of Sz. pombe can successfully replace the homologous ABC10α subunit in nuclear RNA polymerases I-III of S. cerevisiae.
AB - The full-length cDNA of the rpc10+ gene encoding mini-subunit Rpc10, which is common for all three nuclear RNA polymerases of the fission yeast Schizosaccharomyces pombe, was cloned and sequenced. The Rpc10 subunit of Sz. pombe and its homologs from S. cerevisiae and H. sapiens are positively charged proteins with a highly conserved C-terminal region and an invariant zinc-binding domain (Zn-finger) of a typical amino acid composition: YxCx2Cx12RCx2CGxR. Functional tests on heterospecific complementation, using tetrad analysis or plasmid shuffling, showed that the Rpc10 subunit of Sz. pombe can successfully replace the homologous ABC10α subunit in nuclear RNA polymerases I-III of S. cerevisiae.
KW - Common subunits; fission yeast
KW - Nuclear RNA polymerases I-III
KW - Rpc10 gene; heterospecific complementation; zinc-binding domains
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M3 - Article
C2 - 9290054
AN - SCOPUS:0031136992
VL - 23
SP - 447
EP - 448
JO - Bioorganicheskaya Khimiya
JF - Bioorganicheskaya Khimiya
SN - 0132-3423
IS - 5
ER -