Molecular cloning and characterization of cDNA of the rpb10+ gene encoding the smallest subunit of nuclear RNA polymerases of Schizosaccharomyces pombe

G. V. Shpakovski, E. N. Lebedenko

Research output: Contribution to journalReview articlepeer-review

1 Citation (Scopus)

Abstract

The full-length cDNA of the rpc10+ gene encoding mini-subunit Rpc10, which is common for all three nuclear RNA polymerases of the fission yeast Schizosaccharomyces pombe, was cloned and sequenced. The RpclO subunit of Sz. pombe and its homologs from S. cerevisiae and H. sapiens are positively charged proteins with a highly conserved C-terminal region and an invariant zinc-binding domain (Zn-finger) of a typical amino acid composition: YxCx2Cx12RCx2CGxR. Functional tests on heterospecific complementation, using tetrad analysis or plasmid shuffling, showed that the Rpc10 subunit of Sz. pombe can successfully replace the homologous ABC10α subunit in nuclear RNA polymerases I-III of S. cerevisiae.

Original languageEnglish
Pages (from-to)412-418
Number of pages7
JournalRussian Journal of Bioorganic Chemistry
Volume23
Issue number5
Publication statusPublished - May 1997
Externally publishedYes

Keywords

  • Fission yeast, rpc10 gene
  • Heterospecific complementation
  • Nuclear RNA polymerases I-III, common subunits
  • Zinc-binding domains

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry

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