Modulation of human macrophage responses to mycobacterium tuberculosis by silver nanoparticles of different size and surface modification

Srijata Sarkar, Bey Fen Leo, Claudia Carranza, Shu Chen, Cesar Rivas-Santiago, Alexandra E. Porter, Mary P. Ryan, Andrew Gow, Kian Fan Chung, Teresa D. Tetley, Junfeng Jim Zhang, Panos G. Georgopoulos, Pamela A. Ohman-Strickland, Stephan Schwander

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Exposure to silver nanoparticles (AgNP) used in consumer products carries potential health risks including increased susceptibility to infectious pathogens. Systematic assessments of antimicrobial macrophage immune responses in the context of AgNP exposure are important because uptake of AgNP by macrophages may lead to alterations of innate immune cell functions. In this study we examined the effects of exposure to AgNP with different particle sizes (20 and 110 nm diameters) and surface chemistry (citrate or polyvinlypyrrolidone capping) on cellular toxicity and innate immune responses against Mycobacterium tuberculosis (M.tb) by human monocyte-derived macrophages (MDM). Exposures of MDM to AgNP significantly reduced cellular viability, increased IL8 and decreased IL10 mRNA expression. Exposure of M.tb-infected MDM to AgNP suppressed M.tb-induced expression of IL1B, IL10, and TNFA mRNA. Furthermore, M.tb-induced IL-1ß, a cytokine critical for host resistance to M.tb, was inhibited by AgNP but not by carbon black particles indicating that the observed immunosuppressive effects of AgNP are particle specific. Suppressive effects of AgNP on the M.tb-induced host immune responses were in part due to AgNPmediated interferences with the TLR signaling pathways that culminate in the activation of the transcription factor NF-κB. AgNP exposure suppressed M.tb-induced expression of a subset of NF-B mediated genes (CSF2, CSF3, IFNG, IL1A, IL1B, IL6, IL10, TNFA, NFKB1A). In addition, AgNP exposure increased the expression of HSPA1A mRNA and the corresponding stress-induced Hsp72 protein. Up-regulation of Hsp72 by AgNP can suppress M.tb-induced NF-κB activation and host immune responses. The observed ability of AgNP to modulate infectious pathogen-induced immune responses has important public health implications.

Original languageEnglish
Article number0143077
JournalPLoS One
Volume10
Issue number11
DOIs
Publication statusPublished - 1 Nov 2015
Externally publishedYes

Fingerprint

nanosilver
Macrophages
Mycobacterium tuberculosis
Silver
Nanoparticles
Surface treatment
macrophages
Modulation
Interleukin-10
Pathogens
Messenger RNA
immune response
monocytes
Chemical activation
HSP72 Heat-Shock Proteins
Soot
Consumer products
Health risks
Public health
Immunosuppressive Agents

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Sarkar, S., Leo, B. F., Carranza, C., Chen, S., Rivas-Santiago, C., Porter, A. E., ... Schwander, S. (2015). Modulation of human macrophage responses to mycobacterium tuberculosis by silver nanoparticles of different size and surface modification. PLoS One, 10(11), [0143077]. https://doi.org/10.1371/journal.pone.0143077

Modulation of human macrophage responses to mycobacterium tuberculosis by silver nanoparticles of different size and surface modification. / Sarkar, Srijata; Leo, Bey Fen; Carranza, Claudia; Chen, Shu; Rivas-Santiago, Cesar; Porter, Alexandra E.; Ryan, Mary P.; Gow, Andrew; Chung, Kian Fan; Tetley, Teresa D.; Zhang, Junfeng Jim; Georgopoulos, Panos G.; Ohman-Strickland, Pamela A.; Schwander, Stephan.

In: PLoS One, Vol. 10, No. 11, 0143077, 01.11.2015.

Research output: Contribution to journalArticle

Sarkar, S, Leo, BF, Carranza, C, Chen, S, Rivas-Santiago, C, Porter, AE, Ryan, MP, Gow, A, Chung, KF, Tetley, TD, Zhang, JJ, Georgopoulos, PG, Ohman-Strickland, PA & Schwander, S 2015, 'Modulation of human macrophage responses to mycobacterium tuberculosis by silver nanoparticles of different size and surface modification', PLoS One, vol. 10, no. 11, 0143077. https://doi.org/10.1371/journal.pone.0143077
Sarkar, Srijata ; Leo, Bey Fen ; Carranza, Claudia ; Chen, Shu ; Rivas-Santiago, Cesar ; Porter, Alexandra E. ; Ryan, Mary P. ; Gow, Andrew ; Chung, Kian Fan ; Tetley, Teresa D. ; Zhang, Junfeng Jim ; Georgopoulos, Panos G. ; Ohman-Strickland, Pamela A. ; Schwander, Stephan. / Modulation of human macrophage responses to mycobacterium tuberculosis by silver nanoparticles of different size and surface modification. In: PLoS One. 2015 ; Vol. 10, No. 11.
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