Modulating Effect of Matrices with Calcium Phosphate Coating on Cytotoxicity of Strontium Ranelate and Ibandronic Acid In Vitro

Abstract

Strontium ranelate (29 μg/ml) and ibandronic acid (50 μM) produced a cytotoxic effect on rat bone marrow myelokaryocytes in vitro. Strontium ranelate increased the number of myelokaryocytes with signs of necrosis, ibandronic acid increased the number of apoptotic and necrotic cells in the 9-day 2D culture of bone marrow cells on the plastic surface of the wells of culture plates. Co-culturing of the bone marrow with 3D matrices with microarc calcium phosphate coating that simulated bone mineral matrix increased intracellular ROS concentration, but abolished the cytotoxic effect of these drugs.

Original language	English
Pages (from-to)	215-219
Number of pages	5
Journal	Bulletin of Experimental Biology and Medicine
Volume	157
Issue number	2
DOIs	https://doi.org/10.1007/s10517-014-2528-7
Publication status	Published - Jun 2014

Keywords

Apoptosis
Flow cytofluorometry
Necrosis
Rat myelokaryocytes
Reactive oxygen species

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

Access to Document

10.1007/s10517-014-2528-7

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Khlusov, IA., Ryazantseva, N. V., Vengerovskii, A. I., Nechaev, K. A., Yakushina, V. D., Dvornichenko, M. V., Sharkeev, Y. P., Legostayeva, E. V., & Novitskii, V. V. (2014). Modulating Effect of Matrices with Calcium Phosphate Coating on Cytotoxicity of Strontium Ranelate and Ibandronic Acid In Vitro. Bulletin of Experimental Biology and Medicine, 157(2), 215-219. https://doi.org/10.1007/s10517-014-2528-7

Modulating Effect of Matrices with Calcium Phosphate Coating on Cytotoxicity of Strontium Ranelate and Ibandronic Acid In Vitro. / Khlusov, Igor Albertovich; Ryazantseva, N. V.; Vengerovskii, A. I.; Nechaev, K. A.; Yakushina, V. D.; Dvornichenko, M. V.; Sharkeev, Yu P.; Legostayeva, E. V.; Novitskii, V. V.

In: Bulletin of Experimental Biology and Medicine, Vol. 157, No. 2, 06.2014, p. 215-219.

Research output: Contribution to journal › Article › peer-review

Khlusov, IA, Ryazantseva, NV, Vengerovskii, AI, Nechaev, KA, Yakushina, VD, Dvornichenko, MV, Sharkeev, YP, Legostayeva, EV & Novitskii, VV 2014, 'Modulating Effect of Matrices with Calcium Phosphate Coating on Cytotoxicity of Strontium Ranelate and Ibandronic Acid In Vitro', Bulletin of Experimental Biology and Medicine, vol. 157, no. 2, pp. 215-219. https://doi.org/10.1007/s10517-014-2528-7

Khlusov, Igor Albertovich ; Ryazantseva, N. V. ; Vengerovskii, A. I. ; Nechaev, K. A. ; Yakushina, V. D. ; Dvornichenko, M. V. ; Sharkeev, Yu P. ; Legostayeva, E. V. ; Novitskii, V. V. / Modulating Effect of Matrices with Calcium Phosphate Coating on Cytotoxicity of Strontium Ranelate and Ibandronic Acid In Vitro. In: Bulletin of Experimental Biology and Medicine. 2014 ; Vol. 157, No. 2. pp. 215-219.

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abstract = "Strontium ranelate (29 μg/ml) and ibandronic acid (50 μM) produced a cytotoxic effect on rat bone marrow myelokaryocytes in vitro. Strontium ranelate increased the number of myelokaryocytes with signs of necrosis, ibandronic acid increased the number of apoptotic and necrotic cells in the 9-day 2D culture of bone marrow cells on the plastic surface of the wells of culture plates. Co-culturing of the bone marrow with 3D matrices with microarc calcium phosphate coating that simulated bone mineral matrix increased intracellular ROS concentration, but abolished the cytotoxic effect of these drugs.",

keywords = "Apoptosis, Flow cytofluorometry, Necrosis, Rat myelokaryocytes, Reactive oxygen species",

author = "Igor Albertovich Khlusov and Ryazantseva, {N. V.} and Vengerovskii, {A. I.} and Nechaev, {K. A.} and Yakushina, {V. D.} and Dvornichenko, {M. V.} and Sharkeev, {Yu P.} and Legostayeva, {E. V.} and Novitskii, {V. V.}",

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AU - Nechaev, K. A.

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