Abstract
Strontium ranelate (29 μg/ml) and ibandronic acid (50 μM) produced a cytotoxic effect on rat bone marrow myelokaryocytes in vitro. Strontium ranelate increased the number of myelokaryocytes with signs of necrosis, ibandronic acid increased the number of apoptotic and necrotic cells in the 9-day 2D culture of bone marrow cells on the plastic surface of the wells of culture plates. Co-culturing of the bone marrow with 3D matrices with microarc calcium phosphate coating that simulated bone mineral matrix increased intracellular ROS concentration, but abolished the cytotoxic effect of these drugs.
| Original language | English |
|---|---|
| Pages (from-to) | 215-219 |
| Number of pages | 5 |
| Journal | Bulletin of Experimental Biology and Medicine |
| Volume | 157 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Jun 2014 |
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Keywords
- Apoptosis
- Flow cytofluorometry
- Necrosis
- Rat myelokaryocytes
- Reactive oxygen species
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Modulating Effect of Matrices with Calcium Phosphate Coating on Cytotoxicity of Strontium Ranelate and Ibandronic Acid In Vitro. / Khlusov, Igor Albertovich; Ryazantseva, N. V.; Vengerovskii, A. I.; Nechaev, K. A.; Yakushina, V. D.; Dvornichenko, M. V.; Sharkeev, Yu P.; Legostayeva, E. V.; Novitskii, V. V.
In: Bulletin of Experimental Biology and Medicine, Vol. 157, No. 2, 06.2014, p. 215-219.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Modulating Effect of Matrices with Calcium Phosphate Coating on Cytotoxicity of Strontium Ranelate and Ibandronic Acid In Vitro
AU - Khlusov, Igor Albertovich
AU - Ryazantseva, N. V.
AU - Vengerovskii, A. I.
AU - Nechaev, K. A.
AU - Yakushina, V. D.
AU - Dvornichenko, M. V.
AU - Sharkeev, Yu P.
AU - Legostayeva, E. V.
AU - Novitskii, V. V.
PY - 2014/6
Y1 - 2014/6
N2 - Strontium ranelate (29 μg/ml) and ibandronic acid (50 μM) produced a cytotoxic effect on rat bone marrow myelokaryocytes in vitro. Strontium ranelate increased the number of myelokaryocytes with signs of necrosis, ibandronic acid increased the number of apoptotic and necrotic cells in the 9-day 2D culture of bone marrow cells on the plastic surface of the wells of culture plates. Co-culturing of the bone marrow with 3D matrices with microarc calcium phosphate coating that simulated bone mineral matrix increased intracellular ROS concentration, but abolished the cytotoxic effect of these drugs.
AB - Strontium ranelate (29 μg/ml) and ibandronic acid (50 μM) produced a cytotoxic effect on rat bone marrow myelokaryocytes in vitro. Strontium ranelate increased the number of myelokaryocytes with signs of necrosis, ibandronic acid increased the number of apoptotic and necrotic cells in the 9-day 2D culture of bone marrow cells on the plastic surface of the wells of culture plates. Co-culturing of the bone marrow with 3D matrices with microarc calcium phosphate coating that simulated bone mineral matrix increased intracellular ROS concentration, but abolished the cytotoxic effect of these drugs.
KW - Apoptosis
KW - Flow cytofluorometry
KW - Necrosis
KW - Rat myelokaryocytes
KW - Reactive oxygen species
UR - http://apps.webofknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=8&SID=V2MowukGGmShSjkyGzy&page=1&doc=3
U2 - 10.1007/s10517-014-2528-7
DO - 10.1007/s10517-014-2528-7
M3 - Article
C2 - 24958373
AN - SCOPUS:84904304365
VL - 157
SP - 215
EP - 219
JO - Bulletin of Experimental Biology and Medicine
JF - Bulletin of Experimental Biology and Medicine
SN - 0007-4888
IS - 2
ER -