Modulating Effect of Matrices with Calcium Phosphate Coating on Cytotoxicity of Strontium Ranelate and Ibandronic Acid In Vitro

Igor Albertovich Khlusov, N. V. Ryazantseva, A. I. Vengerovskii, K. A. Nechaev, V. D. Yakushina, M. V. Dvornichenko, Yu P. Sharkeev, E. V. Legostayeva, V. V. Novitskii

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Strontium ranelate (29 μg/ml) and ibandronic acid (50 μM) produced a cytotoxic effect on rat bone marrow myelokaryocytes in vitro. Strontium ranelate increased the number of myelokaryocytes with signs of necrosis, ibandronic acid increased the number of apoptotic and necrotic cells in the 9-day 2D culture of bone marrow cells on the plastic surface of the wells of culture plates. Co-culturing of the bone marrow with 3D matrices with microarc calcium phosphate coating that simulated bone mineral matrix increased intracellular ROS concentration, but abolished the cytotoxic effect of these drugs.

Original languageEnglish
Pages (from-to)215-219
Number of pages5
JournalBulletin of Experimental Biology and Medicine
Volume157
Issue number2
DOIs
Publication statusPublished - Jun 2014

Fingerprint

strontium ranelate
Cytotoxicity
Bone
Bone Marrow
Coatings
Bone Matrix
Bone Marrow Cells
Plastics
Minerals
Necrosis
Cell culture
Pharmaceutical Preparations
Rats
Cells
ibandronic acid
calcium phosphate
In Vitro Techniques

Keywords

  • Apoptosis
  • Flow cytofluorometry
  • Necrosis
  • Rat myelokaryocytes
  • Reactive oxygen species

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Modulating Effect of Matrices with Calcium Phosphate Coating on Cytotoxicity of Strontium Ranelate and Ibandronic Acid In Vitro. / Khlusov, Igor Albertovich; Ryazantseva, N. V.; Vengerovskii, A. I.; Nechaev, K. A.; Yakushina, V. D.; Dvornichenko, M. V.; Sharkeev, Yu P.; Legostayeva, E. V.; Novitskii, V. V.

In: Bulletin of Experimental Biology and Medicine, Vol. 157, No. 2, 06.2014, p. 215-219.

Research output: Contribution to journalArticle

Khlusov, Igor Albertovich ; Ryazantseva, N. V. ; Vengerovskii, A. I. ; Nechaev, K. A. ; Yakushina, V. D. ; Dvornichenko, M. V. ; Sharkeev, Yu P. ; Legostayeva, E. V. ; Novitskii, V. V. / Modulating Effect of Matrices with Calcium Phosphate Coating on Cytotoxicity of Strontium Ranelate and Ibandronic Acid In Vitro. In: Bulletin of Experimental Biology and Medicine. 2014 ; Vol. 157, No. 2. pp. 215-219.
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