Modelling of the receptor-ligand interaction in a single cell mode

A. V. Stepanov, J. Xie, M. A. Dronina, V. D. Knorre, A. A. Belogurov, S. M. Deyev, A. G. Gabibov

Research output: Contribution to journalArticle

Abstract

Membrane-associated intrareceptoral and ligand-receptor interactions are involved in intracellular signalling. Community of surface receptors represents extremely important target for modern therapeutics. Their developing evidently requires elaboration of effective screening platforms. In this study we suggest universal platform designed to test receptor-ligand interaction in a "single cell" mode. For this purpose we created a DNA vector utilizing modular structure of chimeric receptors. The whole platform was tested using myc peptide - anti-myc antibody counterpart, which is directly linked with regulation of cancer cell development. In this study we succeeded to obtain Jurkat cells transduced with cDNA coding for chimeric antigenic receptor containing extracellular scFv of the myc-specific antibody and human Fc fused with transmembrane anchor. We showed that chimeric receptor interacts with myc peptide in a "single cell" mode, which in turn leads to the activation of T cells. We further suggest that designed system can be used for any other receptor-ligand pair to detect their interaction directly on the cell surface. Elaborated platform may be applied for the widerange screening of the agents with therapeutic potential on the cell membrane ex vivo.

Original languageEnglish
Pages (from-to)102-109
Number of pages8
JournalBiologicheskie Membrany
Volume32
Issue number2
DOIs
Publication statusPublished - 2015
Externally publishedYes

Fingerprint

Ligands
Peptides
Jurkat Cells
Anti-Idiotypic Antibodies
Complementary DNA
Cell Membrane
T-Lymphocytes
Membranes
Antibodies
DNA
Therapeutics
Neoplasms

Keywords

  • Cell membrane
  • Chimeric antigen T-cell receptor
  • Ligand-receptor interactions
  • Reporter cells

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Modelling of the receptor-ligand interaction in a single cell mode. / Stepanov, A. V.; Xie, J.; Dronina, M. A.; Knorre, V. D.; Belogurov, A. A.; Deyev, S. M.; Gabibov, A. G.

In: Biologicheskie Membrany, Vol. 32, No. 2, 2015, p. 102-109.

Research output: Contribution to journalArticle

Stepanov, AV, Xie, J, Dronina, MA, Knorre, VD, Belogurov, AA, Deyev, SM & Gabibov, AG 2015, 'Modelling of the receptor-ligand interaction in a single cell mode', Biologicheskie Membrany, vol. 32, no. 2, pp. 102-109. https://doi.org/10.7868/S0233475515020097
Stepanov, A. V. ; Xie, J. ; Dronina, M. A. ; Knorre, V. D. ; Belogurov, A. A. ; Deyev, S. M. ; Gabibov, A. G. / Modelling of the receptor-ligand interaction in a single cell mode. In: Biologicheskie Membrany. 2015 ; Vol. 32, No. 2. pp. 102-109.
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