TY - JOUR
T1 - Mineralized self-assembled peptides on 3D laser-made scaffolds
T2 - A new route toward 'scaffold on scaffold' hard tissue engineering
AU - Terzaki, Konstantina
AU - Kalloudi, Erifyli
AU - Mossou, Estelle
AU - Mitchell, Edward P.
AU - Forsyth, V. Trevor
AU - Rosseeva, Elena
AU - Simon, Paul
AU - Vamvakaki, Maria
AU - Chatzinikolaidou, Maria
AU - Mitraki, Anna
AU - Farsari, Maria
N1 - Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.
PY - 2013/12
Y1 - 2013/12
N2 - In this study, we propose a new approach to hard tissue regeneration based on the mineralization of 3D scaffolds made using lasers. To this end, we report the rational design of aspartate-containing self-assembling peptides targeted for calcium binding. We further investigate the suitability of these peptides to support cell attachment and proliferation when coupled on a hybrid organic-inorganic structurable material, and evaluate the response of pre-osteoblastic cells on functionalized 3D scaffolds and material surfaces. Our results show that the mineralized peptide, when immobilized on a hybrid photo-structurable material strongly supports cell adhesion, a proliferation increase after three and seven days in culture, and exhibits a statistically significant increase of biomineralization. We propose this strategy as a 'scaffold on scaffold' approach for hard tissue regeneration.
AB - In this study, we propose a new approach to hard tissue regeneration based on the mineralization of 3D scaffolds made using lasers. To this end, we report the rational design of aspartate-containing self-assembling peptides targeted for calcium binding. We further investigate the suitability of these peptides to support cell attachment and proliferation when coupled on a hybrid organic-inorganic structurable material, and evaluate the response of pre-osteoblastic cells on functionalized 3D scaffolds and material surfaces. Our results show that the mineralized peptide, when immobilized on a hybrid photo-structurable material strongly supports cell adhesion, a proliferation increase after three and seven days in culture, and exhibits a statistically significant increase of biomineralization. We propose this strategy as a 'scaffold on scaffold' approach for hard tissue regeneration.
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U2 - 10.1088/1758-5082/5/4/045002
DO - 10.1088/1758-5082/5/4/045002
M3 - Article
C2 - 23988557
AN - SCOPUS:84889029424
VL - 5
JO - Biofabrication
JF - Biofabrication
SN - 1758-5082
IS - 4
M1 - 045002
ER -