Mineralized self-assembled peptides on 3D laser-made scaffolds: A new route toward 'scaffold on scaffold' hard tissue engineering

Konstantina Terzaki, Erifyli Kalloudi, Estelle Mossou, Edward P. Mitchell, V. Trevor Forsyth, Elena Rosseeva, Paul Simon, Maria Vamvakaki, Maria Chatzinikolaidou, Anna Mitraki, Maria Farsari

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)

Abstract

In this study, we propose a new approach to hard tissue regeneration based on the mineralization of 3D scaffolds made using lasers. To this end, we report the rational design of aspartate-containing self-assembling peptides targeted for calcium binding. We further investigate the suitability of these peptides to support cell attachment and proliferation when coupled on a hybrid organic-inorganic structurable material, and evaluate the response of pre-osteoblastic cells on functionalized 3D scaffolds and material surfaces. Our results show that the mineralized peptide, when immobilized on a hybrid photo-structurable material strongly supports cell adhesion, a proliferation increase after three and seven days in culture, and exhibits a statistically significant increase of biomineralization. We propose this strategy as a 'scaffold on scaffold' approach for hard tissue regeneration.

Original languageEnglish
Article number045002
JournalBiofabrication
Volume5
Issue number4
DOIs
Publication statusPublished - Dec 2013
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Biomaterials
  • Biomedical Engineering

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