Microcapsules functionalized with neuraminidase can enter vascular endothelial cells in vitro

Weizhi Liu, Xiaocong Wang, Ke Bai, Miao Lin, Gleb Sukhorukov, Wen Wang

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Microcapsules made of polyelectrolyte multilayers exhibit no or low toxicity, appropriate mechanical stability, variable controllable degradation and can incorporate remote release mechanisms triggered by various stimuli, making them well suited for targeted drug delivery to live cells. This study investigates interactions between microcapsules made of synthetic (i.e. polystyrenesulfonate sodium salt/polyallylamine hydrochloride) or natural (i.e. dextran sulfate/poly-L-arginine) polyelectrolyte and human umbilical vein endothelial cells with particular focus on the effect of the glycocalyx layer on the intake of microcapsules by endothelial cells. Neuraminidase cleaves N-acetyl neuraminic acid residues of glycoproteins and targets the sialic acid component of the glycocalyx on the cell membrane. Three-dimensional confocal images reveal that microcapsules, functionalized with neuraminidase, can be internalized by endothelial cells. Capsules without neuraminidase are blocked by the glycocalyx layer. Uptake of the microcapsules is most significant in the first 2 h. Following their internalization by endothelial cells, biodegradable DS/PArg capsules rupture by day 5; however, there is no obvious change in the shape and integrity of PSS/PAH capsules within the period of observation. Results from the study support our hypothesis that the glycocalyx functions as an endothelial barrier to cross-membrane movement of microcapsules. Neuraminidase-loaded microcapsules can enter endothelial cells by localized cleavage of glycocalyx components with minimum disruption of the glycocalyx layer and therefore have high potential to act as drug delivery vehicles to reach tissues beyond the endothelial barrier of blood vessels.

Original languageEnglish
Article number20141027
JournalJournal of the Royal Society Interface
Volume11
Issue number101
DOIs
Publication statusPublished - 6 Dec 2014
Externally publishedYes

Fingerprint

Endothelial cells
Neuraminidase
Capsules
Glycocalyx
Endothelial Cells
Polyelectrolytes
Arginine
Glycoproteins
Dextran
Acids
Mechanical stability
Blood vessels
Cell membranes
Polycyclic aromatic hydrocarbons
Drug delivery
Toxicity
Multilayers
Neuraminic Acids
Sodium
In Vitro Techniques

Keywords

  • Biodegradable microcapsules
  • Endothelial glycocalyx
  • Neuraminidase
  • Targeted drug delivery
  • Transport across cell membrane

ASJC Scopus subject areas

  • Biophysics
  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Biomaterials
  • Biochemistry
  • Medicine(all)

Cite this

Microcapsules functionalized with neuraminidase can enter vascular endothelial cells in vitro. / Liu, Weizhi; Wang, Xiaocong; Bai, Ke; Lin, Miao; Sukhorukov, Gleb; Wang, Wen.

In: Journal of the Royal Society Interface, Vol. 11, No. 101, 20141027, 06.12.2014.

Research output: Contribution to journalArticle

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