TY - JOUR
T1 - Microbiological synthesis of methyl ethyl ketone
AU - Astashkina, Anna
AU - Kolbysheva, Yuliya
AU - Nikiforova, Alena
AU - Bakibayev, Abdigali
N1 - Publisher Copyright:
© The Authors, published by EDP Sciences, 2016.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2016/11/17
Y1 - 2016/11/17
N2 - Optimal conditions for the microbiological synthesis of methyl ethyl ketone (MEK) on the mineral medium with butanol-1, butanol-2, 2-methylpropanol-2 by hydrocarbon-oxidizing bacteria Pseudomonas spp. were chosen. Optimal conditions for microbiological synthesis are liquid mineral Adkins' medium containing 3% (vol.) of the substrate, stirring speed-80-100 rev/min, temperature-37° C, and synthesis time 24-48 hours. The specific growth rate and time of culture generation during the microbial synthesis were determined. It was found that methyl ethyl ketone is formed in the reaction mixture after 8, 48 and 72 hours when using 2-methylpropanol-2, butanol-2, butanol-1 as substrates, respectively. Maximum methyl ethyl ketone yield in the reaction mixture is observed after 12 hours in the case of using 2-methylpropanol-2 as a carbon source and accounts for 10%.
AB - Optimal conditions for the microbiological synthesis of methyl ethyl ketone (MEK) on the mineral medium with butanol-1, butanol-2, 2-methylpropanol-2 by hydrocarbon-oxidizing bacteria Pseudomonas spp. were chosen. Optimal conditions for microbiological synthesis are liquid mineral Adkins' medium containing 3% (vol.) of the substrate, stirring speed-80-100 rev/min, temperature-37° C, and synthesis time 24-48 hours. The specific growth rate and time of culture generation during the microbial synthesis were determined. It was found that methyl ethyl ketone is formed in the reaction mixture after 8, 48 and 72 hours when using 2-methylpropanol-2, butanol-2, butanol-1 as substrates, respectively. Maximum methyl ethyl ketone yield in the reaction mixture is observed after 12 hours in the case of using 2-methylpropanol-2 as a carbon source and accounts for 10%.
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U2 - 10.1051/matecconf/20168501022
DO - 10.1051/matecconf/20168501022
M3 - Conference article
AN - SCOPUS:85008479749
VL - 85
JO - MATEC Web of Conferences
JF - MATEC Web of Conferences
SN - 2261-236X
M1 - 01022
T2 - Chemistry and Chemical Technology in XXI Century, CCT 2016
Y2 - 17 May 2016 through 20 May 2016
ER -