Microbiological synthesis of methyl ethyl ketone

Abstract

Optimal conditions for the microbiological synthesis of methyl ethyl ketone (MEK) on the mineral medium with butanol-1, butanol-2, 2-methylpropanol-2 by hydrocarbon-oxidizing bacteria Pseudomonas spp. were chosen. Optimal conditions for microbiological synthesis are liquid mineral Adkins' medium containing 3% (vol.) of the substrate, stirring speed-80-100 rev/min, temperature-37° C, and synthesis time 24-48 hours. The specific growth rate and time of culture generation during the microbial synthesis were determined. It was found that methyl ethyl ketone is formed in the reaction mixture after 8, 48 and 72 hours when using 2-methylpropanol-2, butanol-2, butanol-1 as substrates, respectively. Maximum methyl ethyl ketone yield in the reaction mixture is observed after 12 hours in the case of using 2-methylpropanol-2 as a carbon source and accounts for 10%.

Original language	English
Article number	01022
Journal	MATEC Web of Conferences
Volume	85
DOIs	https://doi.org/10.1051/matecconf/20168501022
Publication status	Published - 17 Nov 2016
Event	Chemistry and Chemical Technology in XXI Century, CCT 2016 - Tomsk, Russian Federation Duration: 17 May 2016 → 20 May 2016

ASJC Scopus subject areas

Chemistry(all)
Materials Science(all)
Engineering(all)

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10.1051/matecconf/20168501022

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@article{08b90c9eefe74a64bc3b042d5e0659a4,

title = "Microbiological synthesis of methyl ethyl ketone",

abstract = "Optimal conditions for the microbiological synthesis of methyl ethyl ketone (MEK) on the mineral medium with butanol-1, butanol-2, 2-methylpropanol-2 by hydrocarbon-oxidizing bacteria Pseudomonas spp. were chosen. Optimal conditions for microbiological synthesis are liquid mineral Adkins' medium containing 3% (vol.) of the substrate, stirring speed-80-100 rev/min, temperature-37° C, and synthesis time 24-48 hours. The specific growth rate and time of culture generation during the microbial synthesis were determined. It was found that methyl ethyl ketone is formed in the reaction mixture after 8, 48 and 72 hours when using 2-methylpropanol-2, butanol-2, butanol-1 as substrates, respectively. Maximum methyl ethyl ketone yield in the reaction mixture is observed after 12 hours in the case of using 2-methylpropanol-2 as a carbon source and accounts for 10%.",

author = "Anna Astashkina and Yuliya Kolbysheva and Alena Nikiforova and Abdigali Bakibayev",

note = "Publisher Copyright: {\textcopyright} The Authors, published by EDP Sciences, 2016. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.; Chemistry and Chemical Technology in XXI Century, CCT 2016 ; Conference date: 17-05-2016 Through 20-05-2016",

year = "2016",

month = nov,

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doi = "10.1051/matecconf/20168501022",

language = "English",

volume = "85",

journal = "MATEC Web of Conferences",

issn = "2261-236X",

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TY - JOUR

T1 - Microbiological synthesis of methyl ethyl ketone

AU - Astashkina, Anna

AU - Kolbysheva, Yuliya

AU - Nikiforova, Alena

AU - Bakibayev, Abdigali

PY - 2016/11/17

Y1 - 2016/11/17

N2 - Optimal conditions for the microbiological synthesis of methyl ethyl ketone (MEK) on the mineral medium with butanol-1, butanol-2, 2-methylpropanol-2 by hydrocarbon-oxidizing bacteria Pseudomonas spp. were chosen. Optimal conditions for microbiological synthesis are liquid mineral Adkins' medium containing 3% (vol.) of the substrate, stirring speed-80-100 rev/min, temperature-37° C, and synthesis time 24-48 hours. The specific growth rate and time of culture generation during the microbial synthesis were determined. It was found that methyl ethyl ketone is formed in the reaction mixture after 8, 48 and 72 hours when using 2-methylpropanol-2, butanol-2, butanol-1 as substrates, respectively. Maximum methyl ethyl ketone yield in the reaction mixture is observed after 12 hours in the case of using 2-methylpropanol-2 as a carbon source and accounts for 10%.

AB - Optimal conditions for the microbiological synthesis of methyl ethyl ketone (MEK) on the mineral medium with butanol-1, butanol-2, 2-methylpropanol-2 by hydrocarbon-oxidizing bacteria Pseudomonas spp. were chosen. Optimal conditions for microbiological synthesis are liquid mineral Adkins' medium containing 3% (vol.) of the substrate, stirring speed-80-100 rev/min, temperature-37° C, and synthesis time 24-48 hours. The specific growth rate and time of culture generation during the microbial synthesis were determined. It was found that methyl ethyl ketone is formed in the reaction mixture after 8, 48 and 72 hours when using 2-methylpropanol-2, butanol-2, butanol-1 as substrates, respectively. Maximum methyl ethyl ketone yield in the reaction mixture is observed after 12 hours in the case of using 2-methylpropanol-2 as a carbon source and accounts for 10%.

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