Mice experimental model of diabetes mellitus type ii based on high fat diet

L. V. Kapilevich, A. N. Zakharova, E. Yu Dyakova, T. A. Kironenko, K. G. Milovanova, J. G. Kalinnikova, A. V. Chibalin

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


Objective: to develop and verify a high fat diet-induced type II diabetes mellitus model. Materials and methods: C57BL/6 male mice were used as the object of study. We developed an experimental diet in which 59% of the total caloric content was from fat. The concentration of glucose in the blood was measured using a portable glucometer PKG-02.4 Satellite Plus (ELTA Company, Russia). Blood samples were obtained by puncture of the caudal vein. Insulin concentrations in the blood plasma of mice were determined by ELISA using the Ultra-sensitive mouse insulin ELISA kit (CrystalChem, USA). Statistical data processing was carried out using the statistical analysis package Statistica 8.0. Results. Mice on a high-fat diet had a significant increase in body weight at week 3. At week 7, there was a significant increase in body weight in mice of the experimental group. The body weight of the experimental animals exceeded that of the control group by 18%. At week 8, the differences in weight rose to 32%. By the end of the experiment, the weight of mice on a high fat diet was 50% higher than the weight of the animals from the control group. It was shown that the applied fatty diet also led to impaired glucose tolerance, and the insulin concentration tripled. Conclusion.The results suggest that the use of high-fat diet in mice leads to an increase in body weight and the formation of obesity, hyperglycemia, decreased glucose tolerance and hyperinsulinemia. All these facts confirm the adequacy of the experimental model for type II diabetes. The created model can be particularly useful in a wide range of studies on insulin resistance, diabetes and obesity, to provide a better understanding of the pathogenesis, as well as to test the effects of therapeutic interventions.

Original languageEnglish
Pages (from-to)53-61
Number of pages9
JournalBulletin of Siberian Medicine
Issue number3
Publication statusPublished - 2019


  • High fat diet
  • Hyperglycemia
  • Insulin resistance
  • Model of diabetes mellitus
  • Type 2 diabetes mellitus

ASJC Scopus subject areas

  • Molecular Medicine

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