Abstract
The in vitro effect of metronidazole on production of active oxygen by neutrophils and in the enzymatic system of glucoseglucose oxidase-peroxidase was studied by luminol-dependent chemiluminescence. An increase in the spontaneous and zymozan-stimulated chemiluminescence and a decrease in the phorbolmyristate acetate (PMA)-stimulated chemiluminescence after 2-hour preincubation of the neutrophils with 8.5 mM of metronidazole were observed. In concentrations of 0.9 to 8.7 mM metronidazole (without washing) dose-dependenly lowered the neutrophil chemiluminescence in response to the effect of PMA and ionophore A23187 and to a lesser degree to that of zymozan. In doses of 20 to 100 mM the drug had an insignificant effect on production of active oxygen by the neutrophils in response to the cell stimulation by PMA, ionophore A23187 and zymozan. The data are in conformity with the scavenger effect of metrinidazole on active oxygen radicals generating in the cell-free enzymatic system both in the presence and in the absence of Superoxide dismutase.
| Original language | English |
|---|---|
| Pages (from-to) | 38-41 |
| Number of pages | 4 |
| Journal | Antibiotiki i Khimioterapiya |
| Volume | 42 |
| Issue number | 8 |
| Publication status | Published - 1997 |
| Externally published | Yes |
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Keywords
- Chemiluminescence
- Ionophore A23187
- Metronidazole
- Neutrophils
- Phorbolmyristate acetate
- Zymozan
ASJC Scopus subject areas
- Microbiology
- Pharmacology
Cite this
Metronidazole effect on active oxygen production by human blood neutrophils. / Shchepetkin, I. A.; Yuriev, S. Yu.
In: Antibiotiki i Khimioterapiya, Vol. 42, No. 8, 1997, p. 38-41.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Metronidazole effect on active oxygen production by human blood neutrophils
AU - Shchepetkin, I. A.
AU - Yuriev, S. Yu
PY - 1997
Y1 - 1997
N2 - The in vitro effect of metronidazole on production of active oxygen by neutrophils and in the enzymatic system of glucoseglucose oxidase-peroxidase was studied by luminol-dependent chemiluminescence. An increase in the spontaneous and zymozan-stimulated chemiluminescence and a decrease in the phorbolmyristate acetate (PMA)-stimulated chemiluminescence after 2-hour preincubation of the neutrophils with 8.5 mM of metronidazole were observed. In concentrations of 0.9 to 8.7 mM metronidazole (without washing) dose-dependenly lowered the neutrophil chemiluminescence in response to the effect of PMA and ionophore A23187 and to a lesser degree to that of zymozan. In doses of 20 to 100 mM the drug had an insignificant effect on production of active oxygen by the neutrophils in response to the cell stimulation by PMA, ionophore A23187 and zymozan. The data are in conformity with the scavenger effect of metrinidazole on active oxygen radicals generating in the cell-free enzymatic system both in the presence and in the absence of Superoxide dismutase.
AB - The in vitro effect of metronidazole on production of active oxygen by neutrophils and in the enzymatic system of glucoseglucose oxidase-peroxidase was studied by luminol-dependent chemiluminescence. An increase in the spontaneous and zymozan-stimulated chemiluminescence and a decrease in the phorbolmyristate acetate (PMA)-stimulated chemiluminescence after 2-hour preincubation of the neutrophils with 8.5 mM of metronidazole were observed. In concentrations of 0.9 to 8.7 mM metronidazole (without washing) dose-dependenly lowered the neutrophil chemiluminescence in response to the effect of PMA and ionophore A23187 and to a lesser degree to that of zymozan. In doses of 20 to 100 mM the drug had an insignificant effect on production of active oxygen by the neutrophils in response to the cell stimulation by PMA, ionophore A23187 and zymozan. The data are in conformity with the scavenger effect of metrinidazole on active oxygen radicals generating in the cell-free enzymatic system both in the presence and in the absence of Superoxide dismutase.
KW - Chemiluminescence
KW - Ionophore A23187
KW - Metronidazole
KW - Neutrophils
KW - Phorbolmyristate acetate
KW - Zymozan
UR - http://www.scopus.com/inward/record.url?scp=0030625845&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030625845&partnerID=8YFLogxK
M3 - Article
VL - 42
SP - 38
EP - 41
JO - Antibiotiki i Khimioterapiya
JF - Antibiotiki i Khimioterapiya
SN - 0235-2990
IS - 8
ER -