Metronidazole effect on active oxygen production by human blood neutrophils

I. A. Shchepetkin, S. Yu Yuriev

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The in vitro effect of metronidazole on production of active oxygen by neutrophils and in the enzymatic system of glucoseglucose oxidase-peroxidase was studied by luminol-dependent chemiluminescence. An increase in the spontaneous and zymozan-stimulated chemiluminescence and a decrease in the phorbolmyristate acetate (PMA)-stimulated chemiluminescence after 2-hour preincubation of the neutrophils with 8.5 mM of metronidazole were observed. In concentrations of 0.9 to 8.7 mM metronidazole (without washing) dose-dependenly lowered the neutrophil chemiluminescence in response to the effect of PMA and ionophore A23187 and to a lesser degree to that of zymozan. In doses of 20 to 100 mM the drug had an insignificant effect on production of active oxygen by the neutrophils in response to the cell stimulation by PMA, ionophore A23187 and zymozan. The data are in conformity with the scavenger effect of metrinidazole on active oxygen radicals generating in the cell-free enzymatic system both in the presence and in the absence of Superoxide dismutase.

Original languageEnglish
Pages (from-to)38-41
Number of pages4
JournalAntibiotiki i Khimioterapiya
Volume42
Issue number8
Publication statusPublished - 1997
Externally publishedYes

Fingerprint

Metronidazole
Luminescence
Reactive Oxygen Species
Neutrophils
Acetates
Ionophores
Calcimycin
Luminol
Cell-Free System
Peroxidase
Superoxide Dismutase
Oxidoreductases
Pharmaceutical Preparations

Keywords

  • Chemiluminescence
  • Ionophore A23187
  • Metronidazole
  • Neutrophils
  • Phorbolmyristate acetate
  • Zymozan

ASJC Scopus subject areas

  • Microbiology
  • Pharmacology

Cite this

Metronidazole effect on active oxygen production by human blood neutrophils. / Shchepetkin, I. A.; Yuriev, S. Yu.

In: Antibiotiki i Khimioterapiya, Vol. 42, No. 8, 1997, p. 38-41.

Research output: Contribution to journalArticle

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