Mesenchymal Stem Cell Magnetization

Magnetic Multilayer Microcapsule Uptake, Toxicity, Impact on Functional Properties, and Perspectives for Magnetic Delivery

Kirill V. Lepik, Albert R. Muslimov, Alexander S. Timin, Vladislav S. Sergeev, Dmitry S. Romanyuk, Ivan S. Moiseev, Elena V. Popova, Igor L. Radchenko, Alexander D. Vilesov, Oleg V. Galibin, Gleb B. Sukhorukov, Boris V. Afanasyev

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Mesenchymal stem cells (MSCs) are widely used in cell therapy due to their convenience, multiline differentiation potential, reproducible protocols, and biological properties. The potential of MSCs to impregnate magnetic microcapsules and their possible influence on cell function and ability to response to magnetic field have been explored. Interestingly, the cells suspended in media show much higher ability in internalization of microcapsules, then MSCs adhere into the surface. There is no significant effect of microcapsules on cell toxicity compared with other cell line-capsule internalization reported in literature. Due to internalization of magnetic capsules by the cells, such cell engineering platform is responsive to external magnetic field, which allows to manipulate MSC migration. Magnetically sorted MSCs are capable to differentiation as confirmed by their conversion to adipogenic and osteogenic cells using standard protocols. There is a minor effect of capsule internalization on cell adhesion, though MSCs are still able to form spheroid made by dozen of thousand MSCs. This work demonstrates the potential of use of microcapsule impregnated MSCs to carry internalized micron-sized vesicles and being navigated with external magnetic signaling.

Original languageEnglish
Pages (from-to)3182-3190
Number of pages9
JournalAdvanced healthcare materials
Volume5
Issue number24
DOIs
Publication statusPublished - 21 Dec 2016

Fingerprint

Magnetic multilayers
Stem cells
Mesenchymal Stromal Cells
Capsules
Toxicity
Magnetization
Magnetic Fields
Cell engineering
Magnetic fields
Cell Engineering
Cell adhesion
Cell- and Tissue-Based Therapy
Cell Adhesion
Cell Movement
Cells
Cell Line

Keywords

  • cell delivery
  • cell therapy
  • magnetic delivery
  • polyelectrolyte capsules
  • stem cells

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Pharmaceutical Science

Cite this

Mesenchymal Stem Cell Magnetization : Magnetic Multilayer Microcapsule Uptake, Toxicity, Impact on Functional Properties, and Perspectives for Magnetic Delivery. / Lepik, Kirill V.; Muslimov, Albert R.; Timin, Alexander S.; Sergeev, Vladislav S.; Romanyuk, Dmitry S.; Moiseev, Ivan S.; Popova, Elena V.; Radchenko, Igor L.; Vilesov, Alexander D.; Galibin, Oleg V.; Sukhorukov, Gleb B.; Afanasyev, Boris V.

In: Advanced healthcare materials, Vol. 5, No. 24, 21.12.2016, p. 3182-3190.

Research output: Contribution to journalArticle

Lepik, KV, Muslimov, AR, Timin, AS, Sergeev, VS, Romanyuk, DS, Moiseev, IS, Popova, EV, Radchenko, IL, Vilesov, AD, Galibin, OV, Sukhorukov, GB & Afanasyev, BV 2016, 'Mesenchymal Stem Cell Magnetization: Magnetic Multilayer Microcapsule Uptake, Toxicity, Impact on Functional Properties, and Perspectives for Magnetic Delivery', Advanced healthcare materials, vol. 5, no. 24, pp. 3182-3190. https://doi.org/10.1002/adhm.201600843
Lepik, Kirill V. ; Muslimov, Albert R. ; Timin, Alexander S. ; Sergeev, Vladislav S. ; Romanyuk, Dmitry S. ; Moiseev, Ivan S. ; Popova, Elena V. ; Radchenko, Igor L. ; Vilesov, Alexander D. ; Galibin, Oleg V. ; Sukhorukov, Gleb B. ; Afanasyev, Boris V. / Mesenchymal Stem Cell Magnetization : Magnetic Multilayer Microcapsule Uptake, Toxicity, Impact on Functional Properties, and Perspectives for Magnetic Delivery. In: Advanced healthcare materials. 2016 ; Vol. 5, No. 24. pp. 3182-3190.
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