Members of the Uncultured Taxon OP1 (“Acetothermia”) Predominate in the Microbial Community of an Alkaline Hot Spring at East-Tuvinian Upland

A. A. Korzhenkov, A. V. Teplyuk, A. V. Lebedinsky, A. A. Khvashchevskaya, Yu G. Kopylova, K. D. Arakchaa, P. N. Golyshin, E. A. Lunev, O. V. Golyshina, I. V. Kublanov, S. V. Toshchakov, S. N. Gavrilov

Research output: Contribution to journalArticle

Abstract

Abstract—: The thermophilic microbial community of a hydrotherm at East-Tuvinian upland is described. High-throughput profiling of the 16S rRNA gene and sequencing of the complete metagenome were used to characterize the phylogenetic and metabolic diversity of the microbial community of the Dikij Arjaan alkaline thermal spring of Ush-Bel’dir mineral water deposit in Tyva, Russia. Members of the uncultured phylum “Acetothermia” (candidate division OP1) predominated in the microbial community of the spring. While this phylum has already been detected in many thermal ecosystems, it never formed the main component of their microbial communities. Apart from “Acetothermia”, members of Nitrospirae and Chloroflexi comprised a significant part of the microbial community. Comparative analysis of our geochemical data on the spring and the previously published data on in silico reconstruction of “Acetothermia” metabolism led us to the suggestion that carbonate and bicarbonate can serve as the major carbon sources for the dominant bacterial group and that “Acetothermia” act as the primary producers in this ecosystem. Analysis of the functional genes also revealed the presence of physiological groups of denitrifyers, iron reducers, carboxydotrophs and diazotrophs. This is the first report on a thermophilic microbial community of a hydrotherm from the southwestern extremity of the Baikal rift zone. Moreover, microbial communities with predominance of uncultured “Acetothermia” have not been reported previously in terrestrial hydrotherms.

Original languageEnglish
Pages (from-to)783-795
Number of pages13
JournalMicrobiology (Russian Federation)
Volume87
Issue number6
DOIs
Publication statusPublished - 1 Nov 2018

Fingerprint

Hot Springs
Ecosystem
Hot Temperature
Chloroflexi
Metagenome
Mineral Waters
Russia
Carbonates
Bicarbonates
rRNA Genes
Computer Simulation
Carbon
Extremities
Iron
Genes

Keywords

  • Acetothermia
  • East-Tuvinian Upland
  • new acetyl-CoA synthases
  • OP1
  • terrestrial hydrotherms
  • uncultured bacteria

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

Members of the Uncultured Taxon OP1 (“Acetothermia”) Predominate in the Microbial Community of an Alkaline Hot Spring at East-Tuvinian Upland. / Korzhenkov, A. A.; Teplyuk, A. V.; Lebedinsky, A. V.; Khvashchevskaya, A. A.; Kopylova, Yu G.; Arakchaa, K. D.; Golyshin, P. N.; Lunev, E. A.; Golyshina, O. V.; Kublanov, I. V.; Toshchakov, S. V.; Gavrilov, S. N.

In: Microbiology (Russian Federation), Vol. 87, No. 6, 01.11.2018, p. 783-795.

Research output: Contribution to journalArticle

Korzhenkov, AA, Teplyuk, AV, Lebedinsky, AV, Khvashchevskaya, AA, Kopylova, YG, Arakchaa, KD, Golyshin, PN, Lunev, EA, Golyshina, OV, Kublanov, IV, Toshchakov, SV & Gavrilov, SN 2018, 'Members of the Uncultured Taxon OP1 (“Acetothermia”) Predominate in the Microbial Community of an Alkaline Hot Spring at East-Tuvinian Upland', Microbiology (Russian Federation), vol. 87, no. 6, pp. 783-795. https://doi.org/10.1134/S0026261718060115
Korzhenkov, A. A. ; Teplyuk, A. V. ; Lebedinsky, A. V. ; Khvashchevskaya, A. A. ; Kopylova, Yu G. ; Arakchaa, K. D. ; Golyshin, P. N. ; Lunev, E. A. ; Golyshina, O. V. ; Kublanov, I. V. ; Toshchakov, S. V. ; Gavrilov, S. N. / Members of the Uncultured Taxon OP1 (“Acetothermia”) Predominate in the Microbial Community of an Alkaline Hot Spring at East-Tuvinian Upland. In: Microbiology (Russian Federation). 2018 ; Vol. 87, No. 6. pp. 783-795.
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