Mediated role of NO-syntase protein kinase C and KATP-channels in realization of cardioprotective impact of cannabinoid HU-210

L. N. Maslov, O. V. Lasukova, A. V. Krylatov, L. Hanuš, Yu B. Lishmanov

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

It was shown that perfusion of the isolated heart of rat with solution containing the CB1- and CB2-receptor agonist HU-210 at concentrations of 0.1 or 1.0 μM/L for a duration of 10 min at 20 min before global ischemia (45 min) and reperfusion (30 min) promotes a twofold decrease in creatine kinase levels in coronary effluent. It was established that KATP channel blockade by glibenclamide (1 μM/L) or inhibition of protein kinase C (2 μM/L) by chelerythrine abolishes the cardioprotective effect of HU-210. The inhibitor of NO synthase L-NAME (1 μM/L) had no effect on the anti-necrotic effect of HU-210. Thus, the intracellular signaling mechanism of the cardioprotective effect of the CB-agonist HU-210 involves the activation of KATP channels and protein kinase C without the participation of NO-synthase.

Original languageEnglish
Pages (from-to)15-18
Number of pages4
JournalEksperimental'naya i Klinicheskaya Farmakologiya
Volume75
Issue number12
Publication statusPublished - 2012

Fingerprint

KATP Channels
Cannabinoids
Protein Kinase C
Nitric Oxide Synthase
Cannabinoid Receptor CB2
Cannabinoid Receptor CB1
Glyburide
NG-Nitroarginine Methyl Ester
Creatine Kinase
Reperfusion
Rats
Effluents
Ischemia
Perfusion
Chemical activation
HU 211

Keywords

  • Cannabinoid receptors
  • Heart
  • Ischemia
  • Reperfusion

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology

Cite this

Mediated role of NO-syntase protein kinase C and KATP-channels in realization of cardioprotective impact of cannabinoid HU-210. / Maslov, L. N.; Lasukova, O. V.; Krylatov, A. V.; Hanuš, L.; Lishmanov, Yu B.

In: Eksperimental'naya i Klinicheskaya Farmakologiya, Vol. 75, No. 12, 2012, p. 15-18.

Research output: Contribution to journalArticle

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AU - Maslov, L. N.

AU - Lasukova, O. V.

AU - Krylatov, A. V.

AU - Hanuš, L.

AU - Lishmanov, Yu B.

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AB - It was shown that perfusion of the isolated heart of rat with solution containing the CB1- and CB2-receptor agonist HU-210 at concentrations of 0.1 or 1.0 μM/L for a duration of 10 min at 20 min before global ischemia (45 min) and reperfusion (30 min) promotes a twofold decrease in creatine kinase levels in coronary effluent. It was established that KATP channel blockade by glibenclamide (1 μM/L) or inhibition of protein kinase C (2 μM/L) by chelerythrine abolishes the cardioprotective effect of HU-210. The inhibitor of NO synthase L-NAME (1 μM/L) had no effect on the anti-necrotic effect of HU-210. Thus, the intracellular signaling mechanism of the cardioprotective effect of the CB-agonist HU-210 involves the activation of KATP channels and protein kinase C without the participation of NO-synthase.

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