Marine polysaccharide-collagen coatings on Ti6Al4V alloy formed by self-assembly

Karl Norris, Oksana I. Mishukova, Agata Zykwinska, Sylvia Colliec-Jouault, Corinne Sinquin, Andrei Koptioug, Stéphane Cuenot, Jemma G. Kerns, Maria A. Surmeneva, Roman A. Surmenev, Timothy E.L. Douglas

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Polysaccharides of marine origin are gaining interest as biomaterial components. Bacteria derived from deep-sea hydrothermal vents can produce sulfated exopolysaccharides (EPS), which can influence cell behavior. The use of such polysaccharides as components of organic, collagen fibril-based coatings on biomaterial surfaces remains unexplored. In this study, collagen fibril coatings enriched with HE800 and GY785 EPS derivatives were deposited on titanium alloy (Ti6Al4V) scaffolds produced by rapid prototyping and subjected to physicochemical and cell biological characterization. Coatings were formed by a self-assembly process whereby polysaccharides were added to acidic collagen molecule solution, followed by neutralization to induced self-assembly of collagen fibrils. Fibril formation resulted in collagen hydrogel formation. Hydrogels formed directly on Ti6Al4V surfaces, and fibrils adsorbed onto the surface. Scanning electron microscopy (SEM) analysis of collagen fibril coatings revealed association of polysaccharides with fibrils. Cell biological characterization revealed good cell adhesion and growth on bare Ti6Al4V surfaces, as well as coatings of collagen fibrils only and collagen fibrils enhanced with HE800 and GY785 EPS derivatives. Hence, the use of both EPS derivatives as coating components is feasible. Further work should focus on cell differentiation.

Original languageEnglish
Article number68
JournalMicromachines
Volume10
Issue number1
DOIs
Publication statusPublished - 19 Jan 2019

Fingerprint

Polysaccharides
Collagen
Self assembly
Coatings
Derivatives
Biomaterials
Hydrogels
Vents
Cell adhesion
Cell growth
Rapid prototyping
Titanium alloys
Scaffolds
Bacteria
Scanning electron microscopy
Molecules

Keywords

  • Collagen
  • Marine exopolysaccharide
  • Surface modification
  • Ti6Al4V

ASJC Scopus subject areas

  • Control and Systems Engineering
  • Mechanical Engineering
  • Electrical and Electronic Engineering

Cite this

Norris, K., Mishukova, O. I., Zykwinska, A., Colliec-Jouault, S., Sinquin, C., Koptioug, A., ... Douglas, T. E. L. (2019). Marine polysaccharide-collagen coatings on Ti6Al4V alloy formed by self-assembly. Micromachines, 10(1), [68]. https://doi.org/10.3390/mi10010068

Marine polysaccharide-collagen coatings on Ti6Al4V alloy formed by self-assembly. / Norris, Karl; Mishukova, Oksana I.; Zykwinska, Agata; Colliec-Jouault, Sylvia; Sinquin, Corinne; Koptioug, Andrei; Cuenot, Stéphane; Kerns, Jemma G.; Surmeneva, Maria A.; Surmenev, Roman A.; Douglas, Timothy E.L.

In: Micromachines, Vol. 10, No. 1, 68, 19.01.2019.

Research output: Contribution to journalArticle

Norris, K, Mishukova, OI, Zykwinska, A, Colliec-Jouault, S, Sinquin, C, Koptioug, A, Cuenot, S, Kerns, JG, Surmeneva, MA, Surmenev, RA & Douglas, TEL 2019, 'Marine polysaccharide-collagen coatings on Ti6Al4V alloy formed by self-assembly', Micromachines, vol. 10, no. 1, 68. https://doi.org/10.3390/mi10010068
Norris K, Mishukova OI, Zykwinska A, Colliec-Jouault S, Sinquin C, Koptioug A et al. Marine polysaccharide-collagen coatings on Ti6Al4V alloy formed by self-assembly. Micromachines. 2019 Jan 19;10(1). 68. https://doi.org/10.3390/mi10010068
Norris, Karl ; Mishukova, Oksana I. ; Zykwinska, Agata ; Colliec-Jouault, Sylvia ; Sinquin, Corinne ; Koptioug, Andrei ; Cuenot, Stéphane ; Kerns, Jemma G. ; Surmeneva, Maria A. ; Surmenev, Roman A. ; Douglas, Timothy E.L. / Marine polysaccharide-collagen coatings on Ti6Al4V alloy formed by self-assembly. In: Micromachines. 2019 ; Vol. 10, No. 1.
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