Lung is the target organ for a monoclonal antibody to angiotensin-converting enzyme

S. M. Danilov, V. R. Muzykantov, A. V. Martynov, Elena Nikolaevna Atochina, I. Y. Sakharov, I. N. Trakht, V. N. Smirnov

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

125I-labeled mouse monoclonal antibody (MoAb) to human angiotensin-converting enzyme (ACE), termed 9B9 and cross-reacting with rat and monkey ACE, when injected into the circulation, accumulates in the lung in up to 10 to 20 greater concentrations than in other organs and blood. That 111In-labeled MoAb 9B9 also accumulates in the lungs of both rats and monkeys very selectively, was clearly revealed by γ-scintigraphy. Unlike polyclonal anti-ACE antibodies that induce an immunodependent lethal reaction when administered intravenously, MoAb 9B9 was well tolerated by rats even at very high doses (up to 300 mg/kg/body weight). At the same time, the administration of this antibody (which does not inhibit the catalytic activity of ACE) resulted in both a 3-fold decrease of the lung ACE activity and an increase in the activity of serum ACE. The highly organ-specific, nondamaging accumulation of the MoAb 9B9 makes it a promising vector for targeted drug delivery to the lung, for modeling of lung pathology, and for γ-scintigraphic visualization of the lung vascular bed. We also suggest that MoAb 9B9 accumulation in the lung may serve as a highly sensitive marker of lung vessel damage upon various lung pathology.

Original languageEnglish
Pages (from-to)118-124
Number of pages7
JournalLaboratory Investigation
Volume64
Issue number1
Publication statusPublished - 1991
Externally publishedYes

Keywords

  • Lung vessel visualization
  • Targeting to the lung

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Fingerprint Dive into the research topics of 'Lung is the target organ for a monoclonal antibody to angiotensin-converting enzyme'. Together they form a unique fingerprint.

  • Cite this

    Danilov, S. M., Muzykantov, V. R., Martynov, A. V., Atochina, E. N., Sakharov, I. Y., Trakht, I. N., & Smirnov, V. N. (1991). Lung is the target organ for a monoclonal antibody to angiotensin-converting enzyme. Laboratory Investigation, 64(1), 118-124.