Lucigenin luminescence elicited by microsomes and its modulation by nitroazole compounds

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Abstract

The lucigenin luminescence elicited by rat liver microsomes and its modulation by the nitroazole compounds metronidazole and sanazole (drug AK- 2123), as well as the rates of lucigen-independent NADPH consumption and cytochrome c reduction, were studied. The obtained data suggest that the luminescence can be the result of univalent lucigenin reduction by microsomal NAD(P)H-reductases, generation of superoxide anion radical in the redox cycle of lucigenin radicals, dioxetane formation by (di)oxygenases, and catalytic action of cytochrome P450 heme on dioxetane decomposition, followed by light emission.

Original languageEnglish
Pages (from-to)499-504
Number of pages6
JournalIUBMB Life
Volume48
Issue number5
Publication statusPublished - 1999
Externally publishedYes

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Keywords

  • Cytochrome P450
  • Lucigenin
  • Luminescence
  • Metronidazole
  • Microsomes
  • Sanazole

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Genetics
  • Molecular Biology

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