Lucigenin luminescence elicited by microsomes and its modulation by nitroazole compounds

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The lucigenin luminescence elicited by rat liver microsomes and its modulation by the nitroazole compounds metronidazole and sanazole (drug AK- 2123), as well as the rates of lucigen-independent NADPH consumption and cytochrome c reduction, were studied. The obtained data suggest that the luminescence can be the result of univalent lucigenin reduction by microsomal NAD(P)H-reductases, generation of superoxide anion radical in the redox cycle of lucigenin radicals, dioxetane formation by (di)oxygenases, and catalytic action of cytochrome P450 heme on dioxetane decomposition, followed by light emission.

Original languageEnglish
Pages (from-to)499-504
Number of pages6
JournalIUBMB Life
Volume48
Issue number5
Publication statusPublished - 1999
Externally publishedYes

Fingerprint

Microsomes
Luminescence
Modulation
Superoxides
Oxygenases
Light emission
Metronidazole
Liver Microsomes
Cytochromes c
Heme
NADP
Liver
NAD
Cytochrome P-450 Enzyme System
Oxidation-Reduction
Rats
Oxidoreductases
Decomposition
Light
Pharmaceutical Preparations

Keywords

  • Cytochrome P450
  • Lucigenin
  • Luminescence
  • Metronidazole
  • Microsomes
  • Sanazole

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Lucigenin luminescence elicited by microsomes and its modulation by nitroazole compounds. / Schepetkin, Igor A.

In: IUBMB Life, Vol. 48, No. 5, 1999, p. 499-504.

Research output: Contribution to journalArticle

@article{b789a710b5e648b0b84501fac50ca1a8,
title = "Lucigenin luminescence elicited by microsomes and its modulation by nitroazole compounds",
abstract = "The lucigenin luminescence elicited by rat liver microsomes and its modulation by the nitroazole compounds metronidazole and sanazole (drug AK- 2123), as well as the rates of lucigen-independent NADPH consumption and cytochrome c reduction, were studied. The obtained data suggest that the luminescence can be the result of univalent lucigenin reduction by microsomal NAD(P)H-reductases, generation of superoxide anion radical in the redox cycle of lucigenin radicals, dioxetane formation by (di)oxygenases, and catalytic action of cytochrome P450 heme on dioxetane decomposition, followed by light emission.",
keywords = "Cytochrome P450, Lucigenin, Luminescence, Metronidazole, Microsomes, Sanazole",
author = "Schepetkin, {Igor A.}",
year = "1999",
language = "English",
volume = "48",
pages = "499--504",
journal = "IUBMB Life",
issn = "1521-6543",
publisher = "Wiley-Blackwell",
number = "5",

}

TY - JOUR

T1 - Lucigenin luminescence elicited by microsomes and its modulation by nitroazole compounds

AU - Schepetkin, Igor A.

PY - 1999

Y1 - 1999

N2 - The lucigenin luminescence elicited by rat liver microsomes and its modulation by the nitroazole compounds metronidazole and sanazole (drug AK- 2123), as well as the rates of lucigen-independent NADPH consumption and cytochrome c reduction, were studied. The obtained data suggest that the luminescence can be the result of univalent lucigenin reduction by microsomal NAD(P)H-reductases, generation of superoxide anion radical in the redox cycle of lucigenin radicals, dioxetane formation by (di)oxygenases, and catalytic action of cytochrome P450 heme on dioxetane decomposition, followed by light emission.

AB - The lucigenin luminescence elicited by rat liver microsomes and its modulation by the nitroazole compounds metronidazole and sanazole (drug AK- 2123), as well as the rates of lucigen-independent NADPH consumption and cytochrome c reduction, were studied. The obtained data suggest that the luminescence can be the result of univalent lucigenin reduction by microsomal NAD(P)H-reductases, generation of superoxide anion radical in the redox cycle of lucigenin radicals, dioxetane formation by (di)oxygenases, and catalytic action of cytochrome P450 heme on dioxetane decomposition, followed by light emission.

KW - Cytochrome P450

KW - Lucigenin

KW - Luminescence

KW - Metronidazole

KW - Microsomes

KW - Sanazole

UR - http://www.scopus.com/inward/record.url?scp=0033391449&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033391449&partnerID=8YFLogxK

M3 - Article

VL - 48

SP - 499

EP - 504

JO - IUBMB Life

JF - IUBMB Life

SN - 1521-6543

IS - 5

ER -