Abstract
The lucigenin luminescence elicited by rat liver microsomes and its modulation by the nitroazole compounds metronidazole and sanazole (drug AK- 2123), as well as the rates of lucigen-independent NADPH consumption and cytochrome c reduction, were studied. The obtained data suggest that the luminescence can be the result of univalent lucigenin reduction by microsomal NAD(P)H-reductases, generation of superoxide anion radical in the redox cycle of lucigenin radicals, dioxetane formation by (di)oxygenases, and catalytic action of cytochrome P450 heme on dioxetane decomposition, followed by light emission.
| Original language | English |
|---|---|
| Pages (from-to) | 499-504 |
| Number of pages | 6 |
| Journal | IUBMB Life |
| Volume | 48 |
| Issue number | 5 |
| Publication status | Published - 1999 |
| Externally published | Yes |
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Keywords
- Cytochrome P450
- Lucigenin
- Luminescence
- Metronidazole
- Microsomes
- Sanazole
ASJC Scopus subject areas
- Biochemistry
- Cell Biology
- Genetics
- Molecular Biology