Abstract
The lucigenin luminescence elicited by rat liver microsomes and its modulation by the nitroazole compounds metronidazole and sanazole (drug AK- 2123), as well as the rates of lucigen-independent NADPH consumption and cytochrome c reduction, were studied. The obtained data suggest that the luminescence can be the result of univalent lucigenin reduction by microsomal NAD(P)H-reductases, generation of superoxide anion radical in the redox cycle of lucigenin radicals, dioxetane formation by (di)oxygenases, and catalytic action of cytochrome P450 heme on dioxetane decomposition, followed by light emission.
| Original language | English |
|---|---|
| Pages (from-to) | 499-504 |
| Number of pages | 6 |
| Journal | IUBMB Life |
| Volume | 48 |
| Issue number | 5 |
| Publication status | Published - 1999 |
| Externally published | Yes |
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Keywords
- Cytochrome P450
- Lucigenin
- Luminescence
- Metronidazole
- Microsomes
- Sanazole
ASJC Scopus subject areas
- Biochemistry
- Cell Biology
- Genetics
- Molecular Biology
Cite this
Lucigenin luminescence elicited by microsomes and its modulation by nitroazole compounds. / Schepetkin, Igor A.
In: IUBMB Life, Vol. 48, No. 5, 1999, p. 499-504.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Lucigenin luminescence elicited by microsomes and its modulation by nitroazole compounds
AU - Schepetkin, Igor A.
PY - 1999
Y1 - 1999
N2 - The lucigenin luminescence elicited by rat liver microsomes and its modulation by the nitroazole compounds metronidazole and sanazole (drug AK- 2123), as well as the rates of lucigen-independent NADPH consumption and cytochrome c reduction, were studied. The obtained data suggest that the luminescence can be the result of univalent lucigenin reduction by microsomal NAD(P)H-reductases, generation of superoxide anion radical in the redox cycle of lucigenin radicals, dioxetane formation by (di)oxygenases, and catalytic action of cytochrome P450 heme on dioxetane decomposition, followed by light emission.
AB - The lucigenin luminescence elicited by rat liver microsomes and its modulation by the nitroazole compounds metronidazole and sanazole (drug AK- 2123), as well as the rates of lucigen-independent NADPH consumption and cytochrome c reduction, were studied. The obtained data suggest that the luminescence can be the result of univalent lucigenin reduction by microsomal NAD(P)H-reductases, generation of superoxide anion radical in the redox cycle of lucigenin radicals, dioxetane formation by (di)oxygenases, and catalytic action of cytochrome P450 heme on dioxetane decomposition, followed by light emission.
KW - Cytochrome P450
KW - Lucigenin
KW - Luminescence
KW - Metronidazole
KW - Microsomes
KW - Sanazole
UR - http://www.scopus.com/inward/record.url?scp=0033391449&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033391449&partnerID=8YFLogxK
M3 - Article
C2 - 10637765
AN - SCOPUS:0033391449
VL - 48
SP - 499
EP - 504
JO - IUBMB Life
JF - IUBMB Life
SN - 1521-6543
IS - 5
ER -