Longtime stability of silver-based SERS substrate in the environment and (bio)environment with variable temperature and humidity

Y. Kalachyova, D. Mares, V. Jerabek, R. Elashnikov, V. Švorčík, O. Lyutakov

Research output: Contribution to journalArticle


The increased involving of Surface Enhanced Raman Spectroscopy (SERS) in the.analytical and bioanalytical field makes this technique commercially available. However, the growing range of available SERS substrates opens the question regarding the stability of SERS substrates during their storage, transportation, and utilization. In this paper, we present systematical study of the stability of SERS enhanced response of SERS-active silver grating under various physical and chemical loads. The silver coated periodical gratings provide excellent enhancement and reproducibility of SERS signal, achieved at the macroscale. The substrate load procedures include the temperature, humidity tests, and their combination. Since the utilization of SERS substrates often involves their interaction with various (bio)environments, the effects of physiological media were studied too. In all cases, the effects of the loads were determined from the changes of SERS effectivity and reproducibility.

Original languageEnglish
Pages (from-to)566-572
Number of pages7
JournalSensors and Actuators, A: Physical
Publication statusPublished - 1 Jan 2019
Externally publishedYes



  • (Bio)environment
  • Humidity
  • SERS
  • Stability
  • Substrate
  • Temperature

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering

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