Lentiviral gene delivery to plasmolipin-expressing cells using Mus caroli endogenous retrovirus envelope protein

M. M. Prokofjeva, G. M. Proshkina, T. D. Lebedev, A. A. Shulgin, P. V. Spirin, V. S. Prassolov, S. M. Deyev

Research output: Contribution to journalReview article

2 Citations (Scopus)

Abstract

Gene therapy is a promising method for treating malignant diseases. One of the main problems is target delivery of therapeutic genes. Here we show that lentiviral vector particles pseudotyped with Mus caroli endogenous retrovirus (McERV) envelope protein can be used for selective transduction of PLLP-expressing cells. As a therapeutic gene in McERV-pseudotyped vector particles we used miniSOG encoding the cytotoxic FMN-binding protein, which can generate reactive oxygen species under illumination. Significant cytotoxic effect (up to 80% of dead cells in population) was observed in PLLP-expressing cells transduced with McERV-pseudotyped vector particles and subjected to illumination. We demonstrated that the McERV-pseudotyped HIV-1 based lentiviral vector particles are an effective tool for selective photoinduced destruction of PLLP-expressing cells.

Original languageEnglish
Pages (from-to)226-233
Number of pages8
JournalBiochimie
Volume142
DOIs
Publication statusPublished - 1 Nov 2017

Keywords

  • Blue light illumination
  • Gene delivery
  • Lentiviral vectors
  • miniSOG
  • Photoinduced cytotoxicity
  • Plasmolipin

ASJC Scopus subject areas

  • Biochemistry

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    Prokofjeva, M. M., Proshkina, G. M., Lebedev, T. D., Shulgin, A. A., Spirin, P. V., Prassolov, V. S., & Deyev, S. M. (2017). Lentiviral gene delivery to plasmolipin-expressing cells using Mus caroli endogenous retrovirus envelope protein. Biochimie, 142, 226-233. https://doi.org/10.1016/j.biochi.2017.09.004