Iterative in situ click chemistry creates antibody-like protein-capture agents

Heather D. Agnew, Rosemary D. Rohde, Steven W. Millward, Arundhati Nag, Woon Seok Yeo, Jason E. Hein, Suresh M. Pitram, V. Abdul Ahad Tariq, Anessa M. Burns, Russell J. Krom, Valery V. Fokin, K. Barry Sharpless, James R. Heath

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

Special agents for protein capture: Iterative in situ click chemistry (see scheme for the tertiary ligand screen) and the one-bead-one-compound method for the creation of a peptide library enable the fragment-based assembly of selective high-affinity protein-capture agents. The resulting ligands are water-soluble and stable chemically, biochemically, and thermally. They can be produced in gram quantities through copper(I)-catalyzed cycloaddition.

Original languageEnglish
Pages (from-to)4944-4948
Number of pages5
JournalAngewandte Chemie - International Edition
Volume48
Issue number27
DOIs
Publication statusPublished - 22 Jun 2009
Externally publishedYes

Fingerprint

Click Chemistry
Antibodies
Ligands
Proteins
Peptide Library
Cycloaddition
Cycloaddition Reaction
Peptides
Copper
Water

Keywords

  • Click chemistry
  • Combinatorial chemistry
  • Inhibitors
  • Peptides
  • Protein-capture agents

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Medicine(all)

Cite this

Agnew, H. D., Rohde, R. D., Millward, S. W., Nag, A., Yeo, W. S., Hein, J. E., ... Heath, J. R. (2009). Iterative in situ click chemistry creates antibody-like protein-capture agents. Angewandte Chemie - International Edition, 48(27), 4944-4948. https://doi.org/10.1002/anie.200900488

Iterative in situ click chemistry creates antibody-like protein-capture agents. / Agnew, Heather D.; Rohde, Rosemary D.; Millward, Steven W.; Nag, Arundhati; Yeo, Woon Seok; Hein, Jason E.; Pitram, Suresh M.; Abdul Ahad Tariq, V.; Burns, Anessa M.; Krom, Russell J.; Fokin, Valery V.; Barry Sharpless, K.; Heath, James R.

In: Angewandte Chemie - International Edition, Vol. 48, No. 27, 22.06.2009, p. 4944-4948.

Research output: Contribution to journalArticle

Agnew, HD, Rohde, RD, Millward, SW, Nag, A, Yeo, WS, Hein, JE, Pitram, SM, Abdul Ahad Tariq, V, Burns, AM, Krom, RJ, Fokin, VV, Barry Sharpless, K & Heath, JR 2009, 'Iterative in situ click chemistry creates antibody-like protein-capture agents', Angewandte Chemie - International Edition, vol. 48, no. 27, pp. 4944-4948. https://doi.org/10.1002/anie.200900488
Agnew, Heather D. ; Rohde, Rosemary D. ; Millward, Steven W. ; Nag, Arundhati ; Yeo, Woon Seok ; Hein, Jason E. ; Pitram, Suresh M. ; Abdul Ahad Tariq, V. ; Burns, Anessa M. ; Krom, Russell J. ; Fokin, Valery V. ; Barry Sharpless, K. ; Heath, James R. / Iterative in situ click chemistry creates antibody-like protein-capture agents. In: Angewandte Chemie - International Edition. 2009 ; Vol. 48, No. 27. pp. 4944-4948.
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