Iterative in situ click chemistry creates antibody-like protein-capture agents

Heather D. Agnew, Rosemary D. Rohde, Steven W. Millward, Arundhati Nag, Woon Seok Yeo, Jason E. Hein, Suresh M. Pitram, V. Abdul Ahad Tariq, Anessa M. Burns, Russell J. Krom, Valery V. Fokin, K. Barry Sharpless, James R. Heath

Research output: Contribution to journalArticlepeer-review

94 Citations (Scopus)

Abstract

Special agents for protein capture: Iterative in situ click chemistry (see scheme for the tertiary ligand screen) and the one-bead-one-compound method for the creation of a peptide library enable the fragment-based assembly of selective high-affinity protein-capture agents. The resulting ligands are water-soluble and stable chemically, biochemically, and thermally. They can be produced in gram quantities through copper(I)-catalyzed cycloaddition.

Original languageEnglish
Pages (from-to)4944-4948
Number of pages5
JournalAngewandte Chemie - International Edition
Volume48
Issue number27
DOIs
Publication statusPublished - 22 Jun 2009
Externally publishedYes

Keywords

  • Click chemistry
  • Combinatorial chemistry
  • Inhibitors
  • Peptides
  • Protein-capture agents

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Medicine(all)

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