Abstract
Design and evaluation of new high-affinity protein compounds that can selectively and efficiently destroy human cancer cells are a priority research area in biomedicine. In this study we report on the ability of the recombinant phototoxic protein DARPin-miniSOG to interact with breast adenacarcinoma human cells overexpressing the extracellular domain of human epidermal growth factor receptor 2 (HER2). It was found that the targeted phototoxin DARPin-miniSOG specifically binds to the HER2 with following internalization and slow recycling back to the cell membrane. An insight into the role of DARPin-miniSOG in HER2 internalization could contribute to the treatment of HER2-positive cancer using this phototoxic protein.
| Original language | English |
|---|---|
| Pages (from-to) | 126-132 |
| Number of pages | 7 |
| Journal | Acta Naturae |
| Volume | 7 |
| Issue number | 3 |
| Publication status | Published - 2015 |
| Externally published | Yes |
Keywords
- DARPin
- HER2
- Internalization
- Recycling
- Scaffold proteins
- Targeted phototoxic protein
ASJC Scopus subject areas
- Biotechnology
- Biochemistry
- Molecular Medicine
- Molecular Biology
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Shilova, O. N., Proshkina, G. M., Lebedenko, E. N., & Deyev, S. M. (2015). Internalization and Recycling of the HER2 Receptor on Human Breast Adenocarcinoma Cells Treated with Targeted Phototoxic Protein DARPinminiSOG. Acta Naturae, 7(3), 126-132.