Internalization and Recycling of the HER2 Receptor on Human Breast Adenocarcinoma Cells Treated with Targeted Phototoxic Protein DARPinminiSOG

O. N. Shilova, G. M. Proshkina, E. N. Lebedenko, S. M. Deyev

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Design and evaluation of new high-affinity protein compounds that can selectively and efficiently destroy human cancer cells are a priority research area in biomedicine. In this study we report on the ability of the recombinant phototoxic protein DARPin-miniSOG to interact with breast adenacarcinoma human cells overexpressing the extracellular domain of human epidermal growth factor receptor 2 (HER2). It was found that the targeted phototoxin DARPin-miniSOG specifically binds to the HER2 with following internalization and slow recycling back to the cell membrane. An insight into the role of DARPin-miniSOG in HER2 internalization could contribute to the treatment of HER2-positive cancer using this phototoxic protein.

Original languageEnglish
Pages (from-to)126-132
Number of pages7
JournalActa Naturae
Volume7
Issue number3
Publication statusPublished - 2015
Externally publishedYes

Keywords

  • DARPin
  • HER2
  • Internalization
  • Recycling
  • Scaffold proteins
  • Targeted phototoxic protein

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Medicine
  • Molecular Biology

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