Interaction study of methyl violet 2B with DNA and voltammetric determination of DNA in aqueous solutions

Eva Horakova, Vlastimil Vyskocil, Jiri Barek

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Differential pulse voltammetry, cyclic voltammetry, and chronocoulometry at a hanging mercury drop electrode were used for the study of the interaction of methyl violet 2B (MV) with double-stranded DNA in 0.1 mol dm-3 acetate buffer pH 4.0. The mechanism of the electrochemical reduction of MV was also investigated, using cyclic voltammetry at a hanging mercury drop electrode. UV-Vis spectrophotometry was used for the investigation of the interaction as well. The kinetic parameters of the reduction of MV and the DNA-MV complex and the thermodynamic parameters of the formed DNA-MV complex were calculated. Moreover, the calibration dependence of the peak current of MV on the concentration of DNA in acetate buffer was constructed to be used for the indirect determination of DNA based on the decreasing peak current of MV with increasing concentration of DNA in the measured solution.

Original languageEnglish
Pages (from-to)119-126
Number of pages8
JournalMonatshefte fur Chemie
Volume147
Issue number1
DOIs
Publication statusPublished - 1 Jan 2016
Externally publishedYes

Fingerprint

Gentian Violet
DNA
Mercury
Cyclic voltammetry
Buffers
Acetates
Electrodes
Spectrophotometry
Kinetic parameters
Thermodynamics
Calibration

Keywords

  • Cyclic voltammetry
  • Differential pulse voltammetry
  • DNA
  • Hanging mercury drop electrode
  • Methyl violet 2B

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Interaction study of methyl violet 2B with DNA and voltammetric determination of DNA in aqueous solutions. / Horakova, Eva; Vyskocil, Vlastimil; Barek, Jiri.

In: Monatshefte fur Chemie, Vol. 147, No. 1, 01.01.2016, p. 119-126.

Research output: Contribution to journalArticle

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