Abstract
Essential oils were obtained by hydrodistillation of the flowers+leaves and stems of Artemisia kotuchovii Kupr. (AKEOf+l and AKEOstm, respectively) and analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). The primary components of the oils were estragole, (E)- and (Z)-β-ocimenes, methyleugenol, limonene, spathulenol, β-pinene, myrcene, and (E)-methyl cinnamate. Seventy-four constituents were present at concentrations from 0.1 to 1.0%, and 34 compounds were identified in trace (stm, but not AKEOf+l, inhibited N-formyl-Met-Leu-Phe (fMLF)-stimulated Ca2+ flux and chemotaxis and phorbol-12-myristate-13-acetate (PMA)-induced reactive oxygen species (ROS) production in human neutrophils. Selected pure constituents, representing >96% of the AKEOstm composition, were also tested in human neutrophils and HL-60 cells transfected with N-formyl peptide receptor 1 (FPR1). One component, 6-methyl-3,5-heptadien-2-one (MHDO), inhibited fMLF- and interleukin 8 (IL-8)-stimulated Ca2+ flux, fMLF-induced chemotaxis, and PMA-induced ROS production in human neutrophils. MHDO also inhibited fMLF-induced Ca2+ flux in FPR1-HL60 cells. These results suggest that MHDO may be effective in modulating some innate immune responses, possibly by inhibition of neutrophil migration and ROS production.
| Original language | English |
|---|---|
| Pages (from-to) | 4999-5007 |
| Number of pages | 9 |
| Journal | Journal of Agricultural and Food Chemistry |
| Volume | 63 |
| Issue number | 20 |
| DOIs | |
| Publication status | Published - 27 May 2015 |
| Externally published | Yes |
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Keywords
- Artemisia kotuchovii
- calcium flux
- chemotaxis
- essential oil
- N-formyl peptide receptor 1 (FPR1)
- neutrophil
- reactive oxygen species
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Chemistry(all)
- Medicine(all)
Cite this
Inhibition of Human Neutrophil Responses by the Essential Oil of Artemisia kotuchovii and Its Constituents. / Schepetkin, Igor A.; Kushnarenko, Svetlana V.; Özek, Gulmira; Kirpotina, Liliya N.; Utegenova, Gulzhakhan A.; Kotukhov, Yuriy A.; Danilova, Alevtina N.; Özek, Temel; Başer, K. Hüsnü Can; Quinn, Mark T.
In: Journal of Agricultural and Food Chemistry, Vol. 63, No. 20, 27.05.2015, p. 4999-5007.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Inhibition of Human Neutrophil Responses by the Essential Oil of Artemisia kotuchovii and Its Constituents
AU - Schepetkin, Igor A.
AU - Kushnarenko, Svetlana V.
AU - Özek, Gulmira
AU - Kirpotina, Liliya N.
AU - Utegenova, Gulzhakhan A.
AU - Kotukhov, Yuriy A.
AU - Danilova, Alevtina N.
AU - Özek, Temel
AU - Başer, K. Hüsnü Can
AU - Quinn, Mark T.
PY - 2015/5/27
Y1 - 2015/5/27
N2 - Essential oils were obtained by hydrodistillation of the flowers+leaves and stems of Artemisia kotuchovii Kupr. (AKEOf+l and AKEOstm, respectively) and analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). The primary components of the oils were estragole, (E)- and (Z)-β-ocimenes, methyleugenol, limonene, spathulenol, β-pinene, myrcene, and (E)-methyl cinnamate. Seventy-four constituents were present at concentrations from 0.1 to 1.0%, and 34 compounds were identified in trace (stm, but not AKEOf+l, inhibited N-formyl-Met-Leu-Phe (fMLF)-stimulated Ca2+ flux and chemotaxis and phorbol-12-myristate-13-acetate (PMA)-induced reactive oxygen species (ROS) production in human neutrophils. Selected pure constituents, representing >96% of the AKEOstm composition, were also tested in human neutrophils and HL-60 cells transfected with N-formyl peptide receptor 1 (FPR1). One component, 6-methyl-3,5-heptadien-2-one (MHDO), inhibited fMLF- and interleukin 8 (IL-8)-stimulated Ca2+ flux, fMLF-induced chemotaxis, and PMA-induced ROS production in human neutrophils. MHDO also inhibited fMLF-induced Ca2+ flux in FPR1-HL60 cells. These results suggest that MHDO may be effective in modulating some innate immune responses, possibly by inhibition of neutrophil migration and ROS production.
AB - Essential oils were obtained by hydrodistillation of the flowers+leaves and stems of Artemisia kotuchovii Kupr. (AKEOf+l and AKEOstm, respectively) and analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). The primary components of the oils were estragole, (E)- and (Z)-β-ocimenes, methyleugenol, limonene, spathulenol, β-pinene, myrcene, and (E)-methyl cinnamate. Seventy-four constituents were present at concentrations from 0.1 to 1.0%, and 34 compounds were identified in trace (stm, but not AKEOf+l, inhibited N-formyl-Met-Leu-Phe (fMLF)-stimulated Ca2+ flux and chemotaxis and phorbol-12-myristate-13-acetate (PMA)-induced reactive oxygen species (ROS) production in human neutrophils. Selected pure constituents, representing >96% of the AKEOstm composition, were also tested in human neutrophils and HL-60 cells transfected with N-formyl peptide receptor 1 (FPR1). One component, 6-methyl-3,5-heptadien-2-one (MHDO), inhibited fMLF- and interleukin 8 (IL-8)-stimulated Ca2+ flux, fMLF-induced chemotaxis, and PMA-induced ROS production in human neutrophils. MHDO also inhibited fMLF-induced Ca2+ flux in FPR1-HL60 cells. These results suggest that MHDO may be effective in modulating some innate immune responses, possibly by inhibition of neutrophil migration and ROS production.
KW - Artemisia kotuchovii
KW - calcium flux
KW - chemotaxis
KW - essential oil
KW - N-formyl peptide receptor 1 (FPR1)
KW - neutrophil
KW - reactive oxygen species
UR - http://www.scopus.com/inward/record.url?scp=84930221709&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84930221709&partnerID=8YFLogxK
U2 - 10.1021/acs.jafc.5b01307
DO - 10.1021/acs.jafc.5b01307
M3 - Article
C2 - 25959257
AN - SCOPUS:84930221709
VL - 63
SP - 4999
EP - 5007
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
SN - 0021-8561
IS - 20
ER -