Inhibition of Human Neutrophil Responses by the Essential Oil of Artemisia kotuchovii and Its Constituents

Igor A. Schepetkin, Svetlana V. Kushnarenko, Gulmira Özek, Liliya N. Kirpotina, Gulzhakhan A. Utegenova, Yuriy A. Kotukhov, Alevtina N. Danilova, Temel Özek, K. Hüsnü Can Başer, Mark T. Quinn

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Essential oils were obtained by hydrodistillation of the flowers+leaves and stems of Artemisia kotuchovii Kupr. (AKEOf+l and AKEOstm, respectively) and analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). The primary components of the oils were estragole, (E)- and (Z)-β-ocimenes, methyleugenol, limonene, spathulenol, β-pinene, myrcene, and (E)-methyl cinnamate. Seventy-four constituents were present at concentrations from 0.1 to 1.0%, and 34 compounds were identified in trace (stm, but not AKEOf+l, inhibited N-formyl-Met-Leu-Phe (fMLF)-stimulated Ca2+ flux and chemotaxis and phorbol-12-myristate-13-acetate (PMA)-induced reactive oxygen species (ROS) production in human neutrophils. Selected pure constituents, representing >96% of the AKEOstm composition, were also tested in human neutrophils and HL-60 cells transfected with N-formyl peptide receptor 1 (FPR1). One component, 6-methyl-3,5-heptadien-2-one (MHDO), inhibited fMLF- and interleukin 8 (IL-8)-stimulated Ca2+ flux, fMLF-induced chemotaxis, and PMA-induced ROS production in human neutrophils. MHDO also inhibited fMLF-induced Ca2+ flux in FPR1-HL60 cells. These results suggest that MHDO may be effective in modulating some innate immune responses, possibly by inhibition of neutrophil migration and ROS production.

Original languageEnglish
Pages (from-to)4999-5007
Number of pages9
JournalJournal of Agricultural and Food Chemistry
Volume63
Issue number20
DOIs
Publication statusPublished - 27 May 2015
Externally publishedYes

Fingerprint

Artemisia
Volatile Oils
Formyl Peptide Receptor
neutrophils
Reactive Oxygen Species
essential oils
methyleugenol
Neutrophils
Fluxes
reactive oxygen species
Gas chromatography
HL-60 Cells
chemotaxis
Acetates
Chemotaxis
calcium
acetates
methyl chavicol
Interleukin-8
cinnamates

Keywords

  • Artemisia kotuchovii
  • calcium flux
  • chemotaxis
  • essential oil
  • N-formyl peptide receptor 1 (FPR1)
  • neutrophil
  • reactive oxygen species

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)
  • Medicine(all)

Cite this

Inhibition of Human Neutrophil Responses by the Essential Oil of Artemisia kotuchovii and Its Constituents. / Schepetkin, Igor A.; Kushnarenko, Svetlana V.; Özek, Gulmira; Kirpotina, Liliya N.; Utegenova, Gulzhakhan A.; Kotukhov, Yuriy A.; Danilova, Alevtina N.; Özek, Temel; Başer, K. Hüsnü Can; Quinn, Mark T.

In: Journal of Agricultural and Food Chemistry, Vol. 63, No. 20, 27.05.2015, p. 4999-5007.

Research output: Contribution to journalArticle

Schepetkin, IA, Kushnarenko, SV, Özek, G, Kirpotina, LN, Utegenova, GA, Kotukhov, YA, Danilova, AN, Özek, T, Başer, KHC & Quinn, MT 2015, 'Inhibition of Human Neutrophil Responses by the Essential Oil of Artemisia kotuchovii and Its Constituents', Journal of Agricultural and Food Chemistry, vol. 63, no. 20, pp. 4999-5007. https://doi.org/10.1021/acs.jafc.5b01307
Schepetkin, Igor A. ; Kushnarenko, Svetlana V. ; Özek, Gulmira ; Kirpotina, Liliya N. ; Utegenova, Gulzhakhan A. ; Kotukhov, Yuriy A. ; Danilova, Alevtina N. ; Özek, Temel ; Başer, K. Hüsnü Can ; Quinn, Mark T. / Inhibition of Human Neutrophil Responses by the Essential Oil of Artemisia kotuchovii and Its Constituents. In: Journal of Agricultural and Food Chemistry. 2015 ; Vol. 63, No. 20. pp. 4999-5007.
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AU - Kushnarenko, Svetlana V.

AU - Özek, Gulmira

AU - Kirpotina, Liliya N.

AU - Utegenova, Gulzhakhan A.

AU - Kotukhov, Yuriy A.

AU - Danilova, Alevtina N.

AU - Özek, Temel

AU - Başer, K. Hüsnü Can

AU - Quinn, Mark T.

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