Incorporation of silver nanoparticles into magnetron-sputtered calcium phosphate layers on titanium as an antibacterial coating

Maria A. Surmeneva, Anna A. Sharonova, Svitlana Chernousova, Oleg Prymak, Kateryna Loza, Mikhail S. Tkachev, Ivan A. Shulepov, Matthias Epple, Roman A. Surmenev

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

A three-layer system of nanocrystalline hydroxyapatite (first layer; 1000 nm thick), silver nanoparticles (second layer; 1.5 μg Ag cm−2) and calcium phosphate (third layer, either 150 or 1000 nm thick) on titanium was prepared by a combination of electrophoretic deposition of silver nanoparticles and the deposition of calcium phosphate by radio frequency magnetron sputtering. Scanning electron microscopy showed that the silver nanoparticles were evenly distributed over the surface. The adhesion of multilayered coating on the substrate was evaluated using the scratch test method. The resistance to cracking and delamination indicated that the multilayered coating has good resistance to contact damage. The release of silver ions from the hydroxyapatite/silver nanoparticle/calcium phosphate system into the phosphate-buffered saline (PBS) solution was measured by atomic absorption spectroscopy (AAS). Approximately one-third of the incorporated silver was released after 3 days immersion into PBS, indicating a total release time of the order of weeks. There were no signs of cracks on the surface of the coating after immersion after various periods, indicating the excellent mechanical stability of the multilayered coating in the physiological environment. An antimicrobial effect against Escherichia coli was found for a 150 nm thick outer layer of the calcium phosphate using a semi-quantitative turbidity test.

Original languageEnglish
Pages (from-to)104-113
Number of pages10
JournalColloids and Surfaces B: Biointerfaces
Volume156
DOIs
Publication statusPublished - 1 Aug 2017

Fingerprint

calcium phosphates
Calcium phosphate
Titanium
Silver
Nanoparticles
titanium
silver
coatings
Coatings
nanoparticles
Immersion
Durapatite
Hydroxyapatite
submerging
phosphates
Phosphates
Atomic spectroscopy
Mechanical stability
turbidity
Turbidity

Keywords

  • Antibacterial coatings
  • Calcium phosphate
  • Coatings
  • Electrophoretic deposition
  • Radio frequency magnetron sputtering
  • Silver

ASJC Scopus subject areas

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Cite this

Incorporation of silver nanoparticles into magnetron-sputtered calcium phosphate layers on titanium as an antibacterial coating. / Surmeneva, Maria A.; Sharonova, Anna A.; Chernousova, Svitlana; Prymak, Oleg; Loza, Kateryna; Tkachev, Mikhail S.; Shulepov, Ivan A.; Epple, Matthias; Surmenev, Roman A.

In: Colloids and Surfaces B: Biointerfaces, Vol. 156, 01.08.2017, p. 104-113.

Research output: Contribution to journalArticle

Surmeneva, Maria A. ; Sharonova, Anna A. ; Chernousova, Svitlana ; Prymak, Oleg ; Loza, Kateryna ; Tkachev, Mikhail S. ; Shulepov, Ivan A. ; Epple, Matthias ; Surmenev, Roman A. / Incorporation of silver nanoparticles into magnetron-sputtered calcium phosphate layers on titanium as an antibacterial coating. In: Colloids and Surfaces B: Biointerfaces. 2017 ; Vol. 156. pp. 104-113.
@article{203dca39110e470fac81a19d349519ab,
title = "Incorporation of silver nanoparticles into magnetron-sputtered calcium phosphate layers on titanium as an antibacterial coating",
abstract = "A three-layer system of nanocrystalline hydroxyapatite (first layer; 1000 nm thick), silver nanoparticles (second layer; 1.5 μg Ag cm−2) and calcium phosphate (third layer, either 150 or 1000 nm thick) on titanium was prepared by a combination of electrophoretic deposition of silver nanoparticles and the deposition of calcium phosphate by radio frequency magnetron sputtering. Scanning electron microscopy showed that the silver nanoparticles were evenly distributed over the surface. The adhesion of multilayered coating on the substrate was evaluated using the scratch test method. The resistance to cracking and delamination indicated that the multilayered coating has good resistance to contact damage. The release of silver ions from the hydroxyapatite/silver nanoparticle/calcium phosphate system into the phosphate-buffered saline (PBS) solution was measured by atomic absorption spectroscopy (AAS). Approximately one-third of the incorporated silver was released after 3 days immersion into PBS, indicating a total release time of the order of weeks. There were no signs of cracks on the surface of the coating after immersion after various periods, indicating the excellent mechanical stability of the multilayered coating in the physiological environment. An antimicrobial effect against Escherichia coli was found for a 150 nm thick outer layer of the calcium phosphate using a semi-quantitative turbidity test.",
keywords = "Antibacterial coatings, Calcium phosphate, Coatings, Electrophoretic deposition, Radio frequency magnetron sputtering, Silver",
author = "Surmeneva, {Maria A.} and Sharonova, {Anna A.} and Svitlana Chernousova and Oleg Prymak and Kateryna Loza and Tkachev, {Mikhail S.} and Shulepov, {Ivan A.} and Matthias Epple and Surmenev, {Roman A.}",
year = "2017",
month = "8",
day = "1",
doi = "10.1016/j.colsurfb.2017.05.016",
language = "English",
volume = "156",
pages = "104--113",
journal = "Colloids and Surfaces B: Biointerfaces",
issn = "0927-7765",
publisher = "Elsevier",

}

TY - JOUR

T1 - Incorporation of silver nanoparticles into magnetron-sputtered calcium phosphate layers on titanium as an antibacterial coating

AU - Surmeneva, Maria A.

AU - Sharonova, Anna A.

AU - Chernousova, Svitlana

AU - Prymak, Oleg

AU - Loza, Kateryna

AU - Tkachev, Mikhail S.

AU - Shulepov, Ivan A.

AU - Epple, Matthias

AU - Surmenev, Roman A.

PY - 2017/8/1

Y1 - 2017/8/1

N2 - A three-layer system of nanocrystalline hydroxyapatite (first layer; 1000 nm thick), silver nanoparticles (second layer; 1.5 μg Ag cm−2) and calcium phosphate (third layer, either 150 or 1000 nm thick) on titanium was prepared by a combination of electrophoretic deposition of silver nanoparticles and the deposition of calcium phosphate by radio frequency magnetron sputtering. Scanning electron microscopy showed that the silver nanoparticles were evenly distributed over the surface. The adhesion of multilayered coating on the substrate was evaluated using the scratch test method. The resistance to cracking and delamination indicated that the multilayered coating has good resistance to contact damage. The release of silver ions from the hydroxyapatite/silver nanoparticle/calcium phosphate system into the phosphate-buffered saline (PBS) solution was measured by atomic absorption spectroscopy (AAS). Approximately one-third of the incorporated silver was released after 3 days immersion into PBS, indicating a total release time of the order of weeks. There were no signs of cracks on the surface of the coating after immersion after various periods, indicating the excellent mechanical stability of the multilayered coating in the physiological environment. An antimicrobial effect against Escherichia coli was found for a 150 nm thick outer layer of the calcium phosphate using a semi-quantitative turbidity test.

AB - A three-layer system of nanocrystalline hydroxyapatite (first layer; 1000 nm thick), silver nanoparticles (second layer; 1.5 μg Ag cm−2) and calcium phosphate (third layer, either 150 or 1000 nm thick) on titanium was prepared by a combination of electrophoretic deposition of silver nanoparticles and the deposition of calcium phosphate by radio frequency magnetron sputtering. Scanning electron microscopy showed that the silver nanoparticles were evenly distributed over the surface. The adhesion of multilayered coating on the substrate was evaluated using the scratch test method. The resistance to cracking and delamination indicated that the multilayered coating has good resistance to contact damage. The release of silver ions from the hydroxyapatite/silver nanoparticle/calcium phosphate system into the phosphate-buffered saline (PBS) solution was measured by atomic absorption spectroscopy (AAS). Approximately one-third of the incorporated silver was released after 3 days immersion into PBS, indicating a total release time of the order of weeks. There were no signs of cracks on the surface of the coating after immersion after various periods, indicating the excellent mechanical stability of the multilayered coating in the physiological environment. An antimicrobial effect against Escherichia coli was found for a 150 nm thick outer layer of the calcium phosphate using a semi-quantitative turbidity test.

KW - Antibacterial coatings

KW - Calcium phosphate

KW - Coatings

KW - Electrophoretic deposition

KW - Radio frequency magnetron sputtering

KW - Silver

UR - http://www.scopus.com/inward/record.url?scp=85019561154&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85019561154&partnerID=8YFLogxK

U2 - 10.1016/j.colsurfb.2017.05.016

DO - 10.1016/j.colsurfb.2017.05.016

M3 - Article

VL - 156

SP - 104

EP - 113

JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

SN - 0927-7765

ER -