Abstract
A three-layer system of nanocrystalline hydroxyapatite (first layer; 1000 nm thick), silver nanoparticles (second layer; 1.5 μg Ag cm−2) and calcium phosphate (third layer, either 150 or 1000 nm thick) on titanium was prepared by a combination of electrophoretic deposition of silver nanoparticles and the deposition of calcium phosphate by radio frequency magnetron sputtering. Scanning electron microscopy showed that the silver nanoparticles were evenly distributed over the surface. The adhesion of multilayered coating on the substrate was evaluated using the scratch test method. The resistance to cracking and delamination indicated that the multilayered coating has good resistance to contact damage. The release of silver ions from the hydroxyapatite/silver nanoparticle/calcium phosphate system into the phosphate-buffered saline (PBS) solution was measured by atomic absorption spectroscopy (AAS). Approximately one-third of the incorporated silver was released after 3 days immersion into PBS, indicating a total release time of the order of weeks. There were no signs of cracks on the surface of the coating after immersion after various periods, indicating the excellent mechanical stability of the multilayered coating in the physiological environment. An antimicrobial effect against Escherichia coli was found for a 150 nm thick outer layer of the calcium phosphate using a semi-quantitative turbidity test.
| Original language | English |
|---|---|
| Pages (from-to) | 104-113 |
| Number of pages | 10 |
| Journal | Colloids and Surfaces B: Biointerfaces |
| Volume | 156 |
| DOIs | |
| Publication status | Published - 1 Aug 2017 |
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Keywords
- Antibacterial coatings
- Calcium phosphate
- Coatings
- Electrophoretic deposition
- Radio frequency magnetron sputtering
- Silver
ASJC Scopus subject areas
- Biotechnology
- Surfaces and Interfaces
- Physical and Theoretical Chemistry
- Colloid and Surface Chemistry
Cite this
Incorporation of silver nanoparticles into magnetron-sputtered calcium phosphate layers on titanium as an antibacterial coating. / Surmeneva, Maria A.; Sharonova, Anna A.; Chernousova, Svitlana; Prymak, Oleg; Loza, Kateryna; Tkachev, Mikhail S.; Shulepov, Ivan A.; Epple, Matthias; Surmenev, Roman A.
In: Colloids and Surfaces B: Biointerfaces, Vol. 156, 01.08.2017, p. 104-113.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Incorporation of silver nanoparticles into magnetron-sputtered calcium phosphate layers on titanium as an antibacterial coating
AU - Surmeneva, Maria A.
AU - Sharonova, Anna A.
AU - Chernousova, Svitlana
AU - Prymak, Oleg
AU - Loza, Kateryna
AU - Tkachev, Mikhail S.
AU - Shulepov, Ivan A.
AU - Epple, Matthias
AU - Surmenev, Roman A.
PY - 2017/8/1
Y1 - 2017/8/1
N2 - A three-layer system of nanocrystalline hydroxyapatite (first layer; 1000 nm thick), silver nanoparticles (second layer; 1.5 μg Ag cm−2) and calcium phosphate (third layer, either 150 or 1000 nm thick) on titanium was prepared by a combination of electrophoretic deposition of silver nanoparticles and the deposition of calcium phosphate by radio frequency magnetron sputtering. Scanning electron microscopy showed that the silver nanoparticles were evenly distributed over the surface. The adhesion of multilayered coating on the substrate was evaluated using the scratch test method. The resistance to cracking and delamination indicated that the multilayered coating has good resistance to contact damage. The release of silver ions from the hydroxyapatite/silver nanoparticle/calcium phosphate system into the phosphate-buffered saline (PBS) solution was measured by atomic absorption spectroscopy (AAS). Approximately one-third of the incorporated silver was released after 3 days immersion into PBS, indicating a total release time of the order of weeks. There were no signs of cracks on the surface of the coating after immersion after various periods, indicating the excellent mechanical stability of the multilayered coating in the physiological environment. An antimicrobial effect against Escherichia coli was found for a 150 nm thick outer layer of the calcium phosphate using a semi-quantitative turbidity test.
AB - A three-layer system of nanocrystalline hydroxyapatite (first layer; 1000 nm thick), silver nanoparticles (second layer; 1.5 μg Ag cm−2) and calcium phosphate (third layer, either 150 or 1000 nm thick) on titanium was prepared by a combination of electrophoretic deposition of silver nanoparticles and the deposition of calcium phosphate by radio frequency magnetron sputtering. Scanning electron microscopy showed that the silver nanoparticles were evenly distributed over the surface. The adhesion of multilayered coating on the substrate was evaluated using the scratch test method. The resistance to cracking and delamination indicated that the multilayered coating has good resistance to contact damage. The release of silver ions from the hydroxyapatite/silver nanoparticle/calcium phosphate system into the phosphate-buffered saline (PBS) solution was measured by atomic absorption spectroscopy (AAS). Approximately one-third of the incorporated silver was released after 3 days immersion into PBS, indicating a total release time of the order of weeks. There were no signs of cracks on the surface of the coating after immersion after various periods, indicating the excellent mechanical stability of the multilayered coating in the physiological environment. An antimicrobial effect against Escherichia coli was found for a 150 nm thick outer layer of the calcium phosphate using a semi-quantitative turbidity test.
KW - Antibacterial coatings
KW - Calcium phosphate
KW - Coatings
KW - Electrophoretic deposition
KW - Radio frequency magnetron sputtering
KW - Silver
UR - http://www.scopus.com/inward/record.url?scp=85019561154&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85019561154&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfb.2017.05.016
DO - 10.1016/j.colsurfb.2017.05.016
M3 - Article
VL - 156
SP - 104
EP - 113
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
SN - 0927-7765
ER -