In vivo X-ray cine-tomography for tracking morphological dynamics

Tomy Dos Santos Rolo, Alexey Ershov, Thomas Van De Kamp, Tilo Baumbach

    Research output: Contribution to journalArticle

    41 Citations (Scopus)

    Abstract

    Scientific cinematography using ultrafast optical imaging is a common tool to study motion. In opaque organisms or structures, X-ray radiography captures sequences of 2D projections to visualize morphological dynamics, but for many applications full fourdimensional (4D) spatiotemporal information is highly desirable. We introduce in vivo X-ray cine-tomography as a 4D imaging technique developed to study real-time dynamics in small living organisms with micrometer spatial resolution and subsecond time resolution. The method enables insights into the physiology of small animals by tracking the 4D morphological dynamics of minute anatomical features as demonstrated in this work by the analysis of fast-moving screw-and-nut-type weevil hip joints. The presented method can be applied to a broad range of biological specimens and biotechnological processes.

    Original languageEnglish
    Pages (from-to)3921-3926
    Number of pages6
    JournalProceedings of the National Academy of Sciences of the United States of America
    Volume111
    Issue number11
    DOIs
    Publication statusPublished - 18 Mar 2014

    Fingerprint

    X Ray Tomography
    Cineradiography
    Weevils
    Nuts
    Optical Imaging
    Hip Joint
    Radiography
    X-Rays

    Keywords

    • In vivo imaging
    • Motion tracking
    • Screw joint
    • Synchrotron

    ASJC Scopus subject areas

    • General

    Cite this

    In vivo X-ray cine-tomography for tracking morphological dynamics. / Rolo, Tomy Dos Santos; Ershov, Alexey; Van De Kamp, Thomas; Baumbach, Tilo.

    In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, No. 11, 18.03.2014, p. 3921-3926.

    Research output: Contribution to journalArticle

    Rolo, Tomy Dos Santos ; Ershov, Alexey ; Van De Kamp, Thomas ; Baumbach, Tilo. / In vivo X-ray cine-tomography for tracking morphological dynamics. In: Proceedings of the National Academy of Sciences of the United States of America. 2014 ; Vol. 111, No. 11. pp. 3921-3926.
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