In vitro assessment of hydroxyapatite coating on the surface of additive manufactured ti6al4v scaffolds

Abstract

Custom orthopedic and dental implants may be fabricated by additive manufacturing (AM), for example using electron beam melting technology. This study is focused on the modification of the surface of Ti6Al4V alloy coin-like scaffolds fabricated via AM technology (EBM®) by radio frequency (RF) magnetron sputter deposition of hydroxyapatite (HA) coating. The scaffolds with HA coating were characterized by Scanning Electron microscopy, X-ray diffraction. HA coating showed a nanocrystalline structure with the crystallites of an average size of 32±9 nm. The ability of the surface to support adhesion and the proliferation of human mesenchymal stem cells was studied using biological short-term tests in vitro. In according to in vitro assessment, thin HA coating stimulated the attachment and proliferation of cells. Human mesenchymal stem cells cultured on the HA-coated scaffold also formed mineralized nodules.

Original language	English
Title of host publication	THERMEC 2016
Publisher	Trans Tech Publications Ltd
Pages	2444-2449
Number of pages	6
Volume	879
ISBN (Print)	9783035711295
DOIs	https://doi.org/10.4028/www.scientific.net/MSF.879.2444
Publication status	Published - 2017
Event	9th International Conference on Processing and Manufacturing of Advanced Materials, THERMEC 2016 - Graz, Austria Duration: 29 May 2016 → 3 Jun 2016

Publication series

Name	Materials Science Forum
Volume	879
ISSN (Print)	0255-5476

Conference

Conference	9th International Conference on Processing and Manufacturing of Advanced Materials, THERMEC 2016
Country	Austria
City	Graz
Period	29.5.16 → 3.6.16

Keywords

Additive manufacturing
Electron beam melting
Hydroxyapatite
Radio frequency (RF) magnetron sputtering

ASJC Scopus subject areas

Materials Science(all)
Condensed Matter Physics
Mechanics of Materials
Mechanical Engineering

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Chudinova, E., Surmeneva, M., Koptioug, A., Savintseva, I., Selezneva, I., Skoglund, P., Syrtanov, M., & Surmenev, R. (2017). In vitro assessment of hydroxyapatite coating on the surface of additive manufactured ti6al4v scaffolds. In THERMEC 2016 (Vol. 879, pp. 2444-2449). (Materials Science Forum; Vol. 879). Trans Tech Publications Ltd. https://doi.org/10.4028/www.scientific.net/MSF.879.2444

In vitro assessment of hydroxyapatite coating on the surface of additive manufactured ti6al4v scaffolds. / Chudinova, Ekaterina ; Surmeneva, Maria ; Koptioug, Andrey; Savintseva, Irina; Selezneva, Irina; Skoglund, Per; Syrtanov, M.; Surmenev, Roman.

THERMEC 2016. Vol. 879 Trans Tech Publications Ltd, 2017. p. 2444-2449 (Materials Science Forum; Vol. 879).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Chudinova, E , Surmeneva, M , Koptioug, A, Savintseva, I, Selezneva, I, Skoglund, P, Syrtanov, M & Surmenev, R 2017, In vitro assessment of hydroxyapatite coating on the surface of additive manufactured ti6al4v scaffolds. in THERMEC 2016. vol. 879, Materials Science Forum, vol. 879, Trans Tech Publications Ltd, pp. 2444-2449, 9th International Conference on Processing and Manufacturing of Advanced Materials, THERMEC 2016, Graz, Austria, 29.5.16. https://doi.org/10.4028/www.scientific.net/MSF.879.2444

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abstract = "Custom orthopedic and dental implants may be fabricated by additive manufacturing (AM), for example using electron beam melting technology. This study is focused on the modification of the surface of Ti6Al4V alloy coin-like scaffolds fabricated via AM technology (EBM{\textregistered}) by radio frequency (RF) magnetron sputter deposition of hydroxyapatite (HA) coating. The scaffolds with HA coating were characterized by Scanning Electron microscopy, X-ray diffraction. HA coating showed a nanocrystalline structure with the crystallites of an average size of 32±9 nm. The ability of the surface to support adhesion and the proliferation of human mesenchymal stem cells was studied using biological short-term tests in vitro. In according to in vitro assessment, thin HA coating stimulated the attachment and proliferation of cells. Human mesenchymal stem cells cultured on the HA-coated scaffold also formed mineralized nodules.",

keywords = "Additive manufacturing, Electron beam melting, Hydroxyapatite, Radio frequency (RF) magnetron sputtering",

author = "Ekaterina Chudinova and Maria Surmeneva and Andrey Koptioug and Irina Savintseva and Irina Selezneva and Per Skoglund and M. Syrtanov and Roman Surmenev",

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AU - Chudinova, Ekaterina

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AU - Selezneva, Irina

AU - Skoglund, Per

AU - Syrtanov, M.

AU - Surmenev, Roman

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AB - Custom orthopedic and dental implants may be fabricated by additive manufacturing (AM), for example using electron beam melting technology. This study is focused on the modification of the surface of Ti6Al4V alloy coin-like scaffolds fabricated via AM technology (EBM®) by radio frequency (RF) magnetron sputter deposition of hydroxyapatite (HA) coating. The scaffolds with HA coating were characterized by Scanning Electron microscopy, X-ray diffraction. HA coating showed a nanocrystalline structure with the crystallites of an average size of 32±9 nm. The ability of the surface to support adhesion and the proliferation of human mesenchymal stem cells was studied using biological short-term tests in vitro. In according to in vitro assessment, thin HA coating stimulated the attachment and proliferation of cells. Human mesenchymal stem cells cultured on the HA-coated scaffold also formed mineralized nodules.

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KW - Electron beam melting

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