In Vitro and in Vivo Visualization and Trapping of Fluorescent Magnetic Microcapsules in a Bloodstream

Denis V. Voronin, Olga A. Sindeeva, Maxim A. Kurochkin, Oksana Mayorova, Ivan V. Fedosov, Oksana Semyachkina-Glushkovskaya, Dmitry A. Gorin, Valery V. Tuchin, Gleb B. Sukhorukov

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Remote navigation and targeted delivery of biologically active compounds is one of the current challenges in the development of drug delivery systems. Modern methods of micro- and nanofabrication give us new opportunities to produce particles and capsules bearing cargo to deploy and possess magnetic properties to be externally navigated. In this work we explore multilayer composite magnetic microcapsules as targeted delivery systems in vitro and in vivo studies under natural conditions of living organism. Herein, we demonstrate magnetic addressing of fluorescent composite microcapsules with embedded magnetite nanoparticles in blood flow environment. First, the visualization and capture of the capsules at the defined blood flow by the magnetic field are shown in vitro in an artificial glass capillary employing a wide-field fluorescence microscope. Afterward, the capsules are visualized and successfully trapped in vivo into externally exposed rat mesentery microvessels. Histological analysis shows that capsules infiltrate small mesenteric vessels whereas large vessels preserve the blood microcirculation. The effect of the magnetic field on capsule preferential localization in bifurcation areas of vasculature, including capsule retention at the site once external magnet is switched off is discussed. The research outcome demonstrates that microcapsules can be effectively addressed in a blood flow, which makes them a promising delivery system with remote navigation by the magnetic field.

Original languageEnglish
Pages (from-to)6885-6893
Number of pages9
JournalACS Applied Materials and Interfaces
Volume9
Issue number8
DOIs
Publication statusPublished - 1 Mar 2017
Externally publishedYes

Fingerprint

Capsules
Blood
Visualization
Magnetic fields
Navigation
Bearings (structural)
Microcirculation
Magnetite nanoparticles
Microfabrication
Composite materials
Nanotechnology
Magnets
Rats
Magnetic properties
Multilayers
Microscopes
Fluorescence
Magnetite Nanoparticles
Glass

Keywords

  • blood flow
  • drug delivery
  • FeO nanoparticles
  • fluorescent magnetic microcapsules
  • rat mesentery
  • wide-field illumination laser microscope

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Voronin, D. V., Sindeeva, O. A., Kurochkin, M. A., Mayorova, O., Fedosov, I. V., Semyachkina-Glushkovskaya, O., ... Sukhorukov, G. B. (2017). In Vitro and in Vivo Visualization and Trapping of Fluorescent Magnetic Microcapsules in a Bloodstream. ACS Applied Materials and Interfaces, 9(8), 6885-6893. https://doi.org/10.1021/acsami.6b15811

In Vitro and in Vivo Visualization and Trapping of Fluorescent Magnetic Microcapsules in a Bloodstream. / Voronin, Denis V.; Sindeeva, Olga A.; Kurochkin, Maxim A.; Mayorova, Oksana; Fedosov, Ivan V.; Semyachkina-Glushkovskaya, Oksana; Gorin, Dmitry A.; Tuchin, Valery V.; Sukhorukov, Gleb B.

In: ACS Applied Materials and Interfaces, Vol. 9, No. 8, 01.03.2017, p. 6885-6893.

Research output: Contribution to journalArticle

Voronin, DV, Sindeeva, OA, Kurochkin, MA, Mayorova, O, Fedosov, IV, Semyachkina-Glushkovskaya, O, Gorin, DA, Tuchin, VV & Sukhorukov, GB 2017, 'In Vitro and in Vivo Visualization and Trapping of Fluorescent Magnetic Microcapsules in a Bloodstream', ACS Applied Materials and Interfaces, vol. 9, no. 8, pp. 6885-6893. https://doi.org/10.1021/acsami.6b15811
Voronin DV, Sindeeva OA, Kurochkin MA, Mayorova O, Fedosov IV, Semyachkina-Glushkovskaya O et al. In Vitro and in Vivo Visualization and Trapping of Fluorescent Magnetic Microcapsules in a Bloodstream. ACS Applied Materials and Interfaces. 2017 Mar 1;9(8):6885-6893. https://doi.org/10.1021/acsami.6b15811
Voronin, Denis V. ; Sindeeva, Olga A. ; Kurochkin, Maxim A. ; Mayorova, Oksana ; Fedosov, Ivan V. ; Semyachkina-Glushkovskaya, Oksana ; Gorin, Dmitry A. ; Tuchin, Valery V. ; Sukhorukov, Gleb B. / In Vitro and in Vivo Visualization and Trapping of Fluorescent Magnetic Microcapsules in a Bloodstream. In: ACS Applied Materials and Interfaces. 2017 ; Vol. 9, No. 8. pp. 6885-6893.
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