In vitro activity assays of sputtered HAp coatings with SiC addition in various simulated biological fluids

Alina Vladescu, Anca Pârâu, Iulian Pana, Cosmin M. Cotrut, Lidia R. Constantin, Viorel Braic, Diana M. Vrânceanu

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


Considering the requirements of medical implantable devices, it is pointed out that biomaterials should play a more sophisticated, longer-term role in the customization and optimization of the material-tissue interface in order to ensure the best long-term clinical outcomes. The aim of this contribution was to assess the performance of silicon carbide-hydroxyapatite in various simulated biological fluids (Dulbecco's modified Eagle's medium (DMEM), simulated body fluid (SBF), and phosphate buffer solution (PBS)) through immersion assays for 21 days at 37 ± 0.5 °C and to evaluate the electrochemical behavior. The coatings were prepared on Ti6Al4V alloy substrates by magnetron sputtering method using two cathodes made of hydroxyapatite and silicon carbide (SiC). After immersion assays the coating's surface was analyzed in terms of morphology, chemical and phase composition, and chemical bonds. According to the electrochemical behavior in the media investigated at 37 ± 0.5 °C, SiC addition inhibits the dissolution of the hydroxyapatite in DMEM acellular media. Furthermore, after adding SiC, the slow degradation of hydroxyapatite in PBS and SBF media as well as biomineralization in DMEM were observed.

Original languageEnglish
Article number389
Issue number6
Publication statusPublished - 1 Jun 2019


  • DMEM
  • Hydroxyapatite
  • Magnetron sputtering
  • PBS
  • SBF
  • Silicon

ASJC Scopus subject areas

  • Surfaces and Interfaces
  • Surfaces, Coatings and Films
  • Materials Chemistry

Fingerprint Dive into the research topics of 'In vitro activity assays of sputtered HAp coatings with SiC addition in various simulated biological fluids'. Together they form a unique fingerprint.

Cite this