Improved stability and efficacy of chitosan/pDNA complexes for gene delivery

Noemi Cifani, Laura Chronopoulou, Barbara Pompili, Antonio Di Martino, Federico Bordi, Simona Sennato, Enea Gino Di Domenico, Cleofe Palocci, Fiorentina Ascenzioni

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Among polymeric polycations, chitosan has emerged as a powerful carrier for gene delivery. Only a few studies have focused on the stability of the chitosan/DNA complex under storage, although this is imperative for nanomedicinal applications. Here, we synthesized polyelectrolyte complexes at a charge ratio of 10 using 50 kDa chitosan and plasmid (p)DNA that encodes a GFP reporter. These preparations were stable up to 3 months at 4 °C and showed reproducible transfection efficiencies in vitro in HEK293 cells. In addition, we developed a methodology that increases the in vitro transfection efficiency of chitosan/pDNA complexes by 150 % with respect to standard procedures. Notably, intracellular pDNA release and transfected cells peaked 5 days following transection of mitotically active cells. These new developments in formulation technology enhance the potential for polymeric nanoparticle-mediated gene therapy.

Original languageEnglish
Pages (from-to)557-565
Number of pages9
JournalBiotechnology Letters
Volume37
Issue number3
DOIs
Publication statusPublished - 1 Jan 2015
Externally publishedYes

Fingerprint

Chitosan
Genes
Transfection
DNA
Gene therapy
HEK293 Cells
Polyelectrolytes
Genetic Therapy
Nanoparticles
Plasmids
Technology
In Vitro Techniques

Keywords

  • Chitosan
  • Chitosan/pDNA nanocomplexes
  • DNA transfection
  • Gene delivery
  • HEK293 cells
  • Therapeutic genes
  • Transfection efficiency

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Cifani, N., Chronopoulou, L., Pompili, B., Di Martino, A., Bordi, F., Sennato, S., ... Ascenzioni, F. (2015). Improved stability and efficacy of chitosan/pDNA complexes for gene delivery. Biotechnology Letters, 37(3), 557-565. https://doi.org/10.1007/s10529-014-1727-7

Improved stability and efficacy of chitosan/pDNA complexes for gene delivery. / Cifani, Noemi; Chronopoulou, Laura; Pompili, Barbara; Di Martino, Antonio; Bordi, Federico; Sennato, Simona; Di Domenico, Enea Gino; Palocci, Cleofe; Ascenzioni, Fiorentina.

In: Biotechnology Letters, Vol. 37, No. 3, 01.01.2015, p. 557-565.

Research output: Contribution to journalArticle

Cifani, N, Chronopoulou, L, Pompili, B, Di Martino, A, Bordi, F, Sennato, S, Di Domenico, EG, Palocci, C & Ascenzioni, F 2015, 'Improved stability and efficacy of chitosan/pDNA complexes for gene delivery', Biotechnology Letters, vol. 37, no. 3, pp. 557-565. https://doi.org/10.1007/s10529-014-1727-7
Cifani, Noemi ; Chronopoulou, Laura ; Pompili, Barbara ; Di Martino, Antonio ; Bordi, Federico ; Sennato, Simona ; Di Domenico, Enea Gino ; Palocci, Cleofe ; Ascenzioni, Fiorentina. / Improved stability and efficacy of chitosan/pDNA complexes for gene delivery. In: Biotechnology Letters. 2015 ; Vol. 37, No. 3. pp. 557-565.
@article{31846fcdda5e49b38a919f9895c8c035,
title = "Improved stability and efficacy of chitosan/pDNA complexes for gene delivery",
abstract = "Among polymeric polycations, chitosan has emerged as a powerful carrier for gene delivery. Only a few studies have focused on the stability of the chitosan/DNA complex under storage, although this is imperative for nanomedicinal applications. Here, we synthesized polyelectrolyte complexes at a charge ratio of 10 using 50 kDa chitosan and plasmid (p)DNA that encodes a GFP reporter. These preparations were stable up to 3 months at 4 °C and showed reproducible transfection efficiencies in vitro in HEK293 cells. In addition, we developed a methodology that increases the in vitro transfection efficiency of chitosan/pDNA complexes by 150 {\%} with respect to standard procedures. Notably, intracellular pDNA release and transfected cells peaked 5 days following transection of mitotically active cells. These new developments in formulation technology enhance the potential for polymeric nanoparticle-mediated gene therapy.",
keywords = "Chitosan, Chitosan/pDNA nanocomplexes, DNA transfection, Gene delivery, HEK293 cells, Therapeutic genes, Transfection efficiency",
author = "Noemi Cifani and Laura Chronopoulou and Barbara Pompili and {Di Martino}, Antonio and Federico Bordi and Simona Sennato and {Di Domenico}, {Enea Gino} and Cleofe Palocci and Fiorentina Ascenzioni",
year = "2015",
month = "1",
day = "1",
doi = "10.1007/s10529-014-1727-7",
language = "English",
volume = "37",
pages = "557--565",
journal = "Biotechnology Letters",
issn = "0141-5492",
publisher = "Springer Netherlands",
number = "3",

}

TY - JOUR

T1 - Improved stability and efficacy of chitosan/pDNA complexes for gene delivery

AU - Cifani, Noemi

AU - Chronopoulou, Laura

AU - Pompili, Barbara

AU - Di Martino, Antonio

AU - Bordi, Federico

AU - Sennato, Simona

AU - Di Domenico, Enea Gino

AU - Palocci, Cleofe

AU - Ascenzioni, Fiorentina

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Among polymeric polycations, chitosan has emerged as a powerful carrier for gene delivery. Only a few studies have focused on the stability of the chitosan/DNA complex under storage, although this is imperative for nanomedicinal applications. Here, we synthesized polyelectrolyte complexes at a charge ratio of 10 using 50 kDa chitosan and plasmid (p)DNA that encodes a GFP reporter. These preparations were stable up to 3 months at 4 °C and showed reproducible transfection efficiencies in vitro in HEK293 cells. In addition, we developed a methodology that increases the in vitro transfection efficiency of chitosan/pDNA complexes by 150 % with respect to standard procedures. Notably, intracellular pDNA release and transfected cells peaked 5 days following transection of mitotically active cells. These new developments in formulation technology enhance the potential for polymeric nanoparticle-mediated gene therapy.

AB - Among polymeric polycations, chitosan has emerged as a powerful carrier for gene delivery. Only a few studies have focused on the stability of the chitosan/DNA complex under storage, although this is imperative for nanomedicinal applications. Here, we synthesized polyelectrolyte complexes at a charge ratio of 10 using 50 kDa chitosan and plasmid (p)DNA that encodes a GFP reporter. These preparations were stable up to 3 months at 4 °C and showed reproducible transfection efficiencies in vitro in HEK293 cells. In addition, we developed a methodology that increases the in vitro transfection efficiency of chitosan/pDNA complexes by 150 % with respect to standard procedures. Notably, intracellular pDNA release and transfected cells peaked 5 days following transection of mitotically active cells. These new developments in formulation technology enhance the potential for polymeric nanoparticle-mediated gene therapy.

KW - Chitosan

KW - Chitosan/pDNA nanocomplexes

KW - DNA transfection

KW - Gene delivery

KW - HEK293 cells

KW - Therapeutic genes

KW - Transfection efficiency

UR - http://www.scopus.com/inward/record.url?scp=84925491258&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84925491258&partnerID=8YFLogxK

U2 - 10.1007/s10529-014-1727-7

DO - 10.1007/s10529-014-1727-7

M3 - Article

C2 - 25388452

AN - SCOPUS:84925491258

VL - 37

SP - 557

EP - 565

JO - Biotechnology Letters

JF - Biotechnology Letters

SN - 0141-5492

IS - 3

ER -