Implantation of track-etched membranes covered by prenatal stromal cells for experimental bullous keratopathy treatment

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A study on the use of track etched membranes (TM) based on the polyethylene terephthalate (PET), including those modified with cold plasma, followed by layering of prenatal stromal cell (PSC) culture on the material surface in vitro in the surgical treatment of a bullous keratopathy (BK) of the cornea has been conducted. The study was conducted on 16 Sylvilagus bachmani rabbits, which after BK modelling were divided into 4 groups: the 1st group was a control group of 4 animals (4 eyes); the 2nd group was a group of 4 animals (4 eyes) which were implanted by TM; the 3rd group was a group of 4 animals (4 eyes) which were implanted by TM with cells; the 4th group was a group of 4 animals (4 eyes) which were implanted by plasma modified TM with cells. TM was obtained by irradiating PET with 40Ar+8 ions and subsequent chemical etching. Eyes were enucleated for histological examination after 8 weeks from the start of the experiment. As a result of the research, it was found that the implantation of TM with a preliminary layering of human PSC promotes the growth of the fibroblast population in the cornea stroma and forming of the leukocyte (lymphocytic and eosinophilic-basophilic) infiltration compared with the implantation of TM without a cellular component. In addition the implantation of TM contributes to a twofold decrease in the induced by BK cornea edema.

Original languageEnglish
Title of host publicationProceedings of the International Conference on Advanced Materials with Hierarchical Structure for New Technologies and Reliable Structures 2019
EditorsVictor E. Panin, Sergey G. Psakhie, Vasily M. Fomin
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735419124
DOIs
Publication statusPublished - 19 Nov 2019
EventInternational Conference on Advanced Materials with Hierarchical Structure for New Technologies and Reliable Structures 2019 - Tomsk, Russian Federation
Duration: 1 Oct 20195 Oct 2019

Publication series

NameAIP Conference Proceedings
Volume2167
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

ConferenceInternational Conference on Advanced Materials with Hierarchical Structure for New Technologies and Reliable Structures 2019
CountryRussian Federation
CityTomsk
Period1.10.195.10.19

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ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Filippova, E. O., Pichugin, V. F., & Khlusov, I. A. (2019). Implantation of track-etched membranes covered by prenatal stromal cells for experimental bullous keratopathy treatment. In V. E. Panin, S. G. Psakhie, & V. M. Fomin (Eds.), Proceedings of the International Conference on Advanced Materials with Hierarchical Structure for New Technologies and Reliable Structures 2019 [020105] (AIP Conference Proceedings; Vol. 2167). American Institute of Physics Inc.. https://doi.org/10.1063/1.5131972