Immunomodulatory and hemagglutinating activities of acidic polysaccharides isolated from Combretum racemosum

Igor A. Schepetkin, Koffi Kouakou, Ahoua Yapi, Liliya N. Kirpotina, Mark A. Jutila, Mark T. Quinn

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Extracts of leaves of different species of the genus Combretum have been used historically to treat a variety of medicinal problems. However, little is known about the active components conferring therapeutic properties to these extracts. In the present studies, we evaluated biochemical properties and immunomodulatory activity of polysaccharides isolated from the leaves of Combretum racemosum. Water-soluble polysaccharides from leaves of C. racemosum were extracted and fractionated by DEAE-cellulose and Diaion HP-20 to obtain a Diaion-bound fraction, designated Combretum polysaccharide-acidic bound or CP-AB, which was eluted with methanol, and an unbound fraction, designated as CP-AU. Molecular weight determination, sugar analysis, and other physical and chemical characterization of the fractions were performed. Fraction CP-AU (mol. weight 5.0 kDa) contained type II arabinogalactan and had potent immunomodulatory activity, inducing the production of interleukin (IL)-1β, -6, -10, and tumor necrosis factor-α (TNF-α) by human peripheral blood mononuclear cells (PBMC) and MonoMac-6 monocytic cells. Likewise, intraperitoneal administration of CP-AU increased in vivo serum levels of IL-6 and monocyte chemoattractant protein-1 (MCP-1) in mice. CP-AU-induced secretion of TNF-α in PBMC was prevented by Toll-like receptor 4 (TLR4) antagonist LPS-RS. Treatment with CP-AU induced phosphorylation of Akt2, Akt3, GSK-3β, HSP27, mTOR, and all p38 MAPK isoforms (α, β, δ, and γ), as well as stimulation of AP-1/NF-κB transcriptional activity. In addition, CP-AU effectively agglutinated erythrocytes from several species, including human, mouse, and rabbit. In contrast, fraction CP-AB was inactive in all biological tests, including cytokine production and hemagglutination. These data suggest that at least part of the beneficial therapeutic effects reported for the water extracts of leaves from C. racemosum are due to modulation of leukocyte functions.

Original languageEnglish
Pages (from-to)628-637
Number of pages10
JournalInternational Immunopharmacology
Volume15
Issue number3
DOIs
Publication statusPublished - Mar 2013
Externally publishedYes

Fingerprint

Combretum
Polysaccharides
Interleukin-6
Blood Cells
Glycogen Synthase Kinase 3
DEAE-Cellulose
Toll-Like Receptor 4
Water
Transcription Factor AP-1
Hemagglutination
Therapeutic Uses
p38 Mitogen-Activated Protein Kinases
Interleukin-1
Methanol
Protein Isoforms
Leukocytes
Tumor Necrosis Factor-alpha
Erythrocytes
Molecular Weight
Phosphorylation

Keywords

  • Combretum racemosum
  • Cytokines
  • Hemagglutination
  • Immunomodulation
  • Macrophage
  • Polysaccharide

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Pharmacology

Cite this

Immunomodulatory and hemagglutinating activities of acidic polysaccharides isolated from Combretum racemosum. / Schepetkin, Igor A.; Kouakou, Koffi; Yapi, Ahoua; Kirpotina, Liliya N.; Jutila, Mark A.; Quinn, Mark T.

In: International Immunopharmacology, Vol. 15, No. 3, 03.2013, p. 628-637.

Research output: Contribution to journalArticle

Schepetkin, Igor A. ; Kouakou, Koffi ; Yapi, Ahoua ; Kirpotina, Liliya N. ; Jutila, Mark A. ; Quinn, Mark T. / Immunomodulatory and hemagglutinating activities of acidic polysaccharides isolated from Combretum racemosum. In: International Immunopharmacology. 2013 ; Vol. 15, No. 3. pp. 628-637.
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